Study of novel embryonic therapy using embryonic circulating mesenchymal stem cells

• Project/Area Number: 24890077
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: University of Fukui
• Principal Investigator: CHINO Takenao 福井大学, 医学部附属病院, 医員 (20521397)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨髄間葉系幹細胞 / マウス胎仔治療 / 間葉系幹細胞 / 末梢血液 / マウス胎仔 / 遺伝子治療

Research Abstract

We get a FACS analysis method taking a little blood sample from alive mouse embryo and neonate. By this method, we comfirmed that Lin-PDGFRa+ cells don't exisit in the mouse neonatal peripheral blood. Adult mouse bone marrow cells are analyzed by FACS that Lin-PDGFRa+ckit-Sca1+ cells are only 0.05-0.1%. And SSEA3 is expressed after separeted from bone marrow. Lin-PGDFRa+ckit-Sca1+SSEA3+ cells are capable of inducing keratinocyte phenotype and expressing K5.