Investigation of the function of nucleostemin in oral squamous cell carcinoma and develop the new therapeutic approoach

• Project/Area Number: 24890173
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kumamoto University
• Principal Investigator: YOSHIDA Ryoji 熊本大学, 医学部附属病院, 医員 (10632458)
• Co-Investigator(Kenkyū-buntansha): SHINOHARA Masanori 熊本大学, 大学院生命科学研究部 歯科口腔外科学分野, 教授 (90117127) ; ITO Takaaki 熊本大学, 大学院生命科学研究部 機能病理学分野, 教授 (70168392) ; NAKAYAMA Hideki 熊本大学, 大学院生命科学研究部 歯科口腔外科学分野, 講師 (70381001)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口腔癌 / 癌幹細胞 / 口腔扁平上皮癌

Research Abstract

The purpose of this study was to explore the impact of Nucleostemin (NS) on malignancy,its clinical significance in oral squamous cell carcinoma (OSCC) patients and develop new therapeutic approach. We investigated the effects of NS on the proliferation and invasion of OSCC using NS-overexpressing or -knockdown OSCC cells. An immunohistochemical analysis of NS was performed in OSCC patients. The expression status of NS significantly correlated to the malignant phenotypes of OSCC cells. The alterations of these malignant phenotypes were associated with the activation of STAT3 signaling and its downstream targets. An immunohistochemical analysis demonstrated that a high NS tumor expression level significantly correlated with an advanced T-stage and nodal status. Furthermore, a Cox regression analysis revealed that the NS status was a significant progression factor for OSCC patients.Our results suggest that targeting NS may provide a promising treatment for highly malignant OSCC.

Research Products

• [Presentation] Overexpression of Nucleostemin contributes to malignant phenotype and poor prognosis in oral squamous cell carcinoma (2013)
  • Author(s): Yoshida R, Nakayama H, Nagata M, Hirosue A, Kawahara K, Nakagawa Y, Hiraki A, Ito T, Shinohara M
  • Organizer: 21st International conference on oral and maxillofacial surgery
  • Place of Presentation: Palau de Congressos, Barcelona, Spain
  • Year and Date: 2013-10-23
• [Presentation] Nucleosteminは口腔扁平上皮癌において増殖、浸潤を制御している (2013)
  • Author(s): 吉田遼司,中山秀,永田将士,田中拓也,川原健太,中川純泰,小島拓,松岡祐一郎,廣末晃之,吉武義泰,平木昭光，篠原正徳
  • Organizer: 第37回頭頸部癌学会
  • Place of Presentation: 京王プラザ（東京）
• [Presentation] Overexpression of Nucleostemin contributes to malignant phenotype and poor prognosis in oral squamous cell carcinoma (2013)
  • Author(s): Yoshida R,Nakayama H,Nagata M,Hirosue A,Kawahara K,Nakagawa Y,Hiraki A,Ito T,Shinohara M
  • Organizer: 21ST INTERNATIONAL CONFERENCE ON ORAL AND MAXILLOFACIAL SURGERY
  • Place of Presentation: Palau de Congressos, Barcelona, Spain
• [Presentation] The possible impacts of nucleostemin on cancer stem cells in oral squamous cell carcinoma (2012)
  • Author(s): Ryoji Yoshida, Hideki Nakayama, Masashi Nagata, Akiyuki Hirosue, Takuya Tanaka, Kenta Kawahara, Yoshihiro Nakagawa, Yoshihiro Yoshitake, Kazutoshi Ota, Akimitsu Hiraki, Takaaki Ito, Masanori Shinohara
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌、北海道
  • Year and Date: 2012-09-20
• [Presentation] 舌扁平上皮癌における核小体蛋白質Nucleosteminの発現と機能 (2012)
  • Author(s): 吉田遼司、中山秀樹、永田将士、田中拓也、川原健太、中川純泰、廣末晃之、吉武義泰、太田和俊、平木昭光、伊藤隆明、篠原正徳
  • Organizer: 第37回頭頸部癌学会
  • Place of Presentation: 島根県民会館、島根
  • Year and Date: 2012-06-07
• [Presentation] The possible impacts of nucleostemin on cancer stem cells in oral squamous cell carcinoma (2012)
  • Author(s): 吉田遼司
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（北海道）