The elucidation of the neurotoxic and prion-like propagative molecular mechanism of alpha synuclein and the clinical application
| Project/Area Number |
24890197
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TATEBE Harutsugu 京都府立医科大学, 医学(系)研究科(研究院), 助教 (00637027)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | アルファ・シヌクレイン / 髄液 / オリゴマー / モノマー / α-synuclein / 線維 / バイオマーカー / CSF / パーキンソン病 / αシヌクレイン / オートファジー |
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| Research Abstract |
alpha-Synuclein(a-syn) is the major pathological component of synucleinopathies including Parkinson's disease.In primary mouse hippocampal neuronal cultures, a-syn-positive synapses were colocalized with the excitatory synapse marker vesicular glutamate transporter-1.
When a-syn was evaluated with non-denaturing gels or size exclusion chromatography (SEC) columns, a-syn monomers migrated as 50- to 60-kDa proteins; however, in denaturing gels, they migrated as 15-kDa proteins. The disulfide-linked dimer and oligomer migrated slower than either the unfolded recombinant a-syn or the native a-syn in SEC.As a result,a-Syn exists predominantly as a disordered monomer in CSF.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Different expression of alpha- synuclein in hippocampal neurons.2014
Author(s)
Taguchi, K, Watanabe, Y, Tsujimura, A, Tatebe, H, Miyata, S, Tokuda, T, Mizuno, T, Tanaka, M
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Journal Title
PLos One
Volume: 9
Issue: 2
Pages: e89327-e89327
DOI
Related Report
Peer Reviewed / Open Access
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