| Project/Area Number |
25251009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Kenji 大阪大学, たんぱく質研究所, 准教授 (20342751)
ICHIKI Tamaki 独立行政法人農業・食品産業技術総合研究機構, 遺伝資源研究センター, 主任研究員 (70355501)
MIYAZAKI Naoyuki 大阪大学, たんぱく質研究所, 特任助教 (00634677)
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| Co-Investigator(Renkei-kenkyūsha) |
HIGASHIURA Akifumi 大阪大学, たんぱく質研究所, 特任助教 (90598129)
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| Research Collaborator |
NAKAMICHI Yusuke 大阪大学, たんぱく質研究所, 特任研究員 (20751217)
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| Project Period (FY) |
2013-05-31 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2015: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2014: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2013: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
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| Keywords | ウイルス / 分子認識と相互作用 / Ｘ線結晶構造解析 / 電子顕微鏡 / ウイルスの細胞内動態 / 分子認識および相互作用 / 放射光 / クライオ電子顕微鏡 / 電子顕微鏡トモグラフィー |
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| Outline of Final Research Achievements |
This project aimed to understand the life cycle of Rice dwarf virus, which is the causal agent of rice dwarf disease, by various techniques including structural biology, biochemistry, and virology. We have succeeded to understand the hierarchical mechanism of self-assembly of virus particle, structure determination of viral proteins and major factors that are involved in virus-host interactions, infection, and multiplication. We have also succeeded to find the best condition for high resolution X-ray diffraction data collection and asymmetrical structure determination by cryo-electron microscopy. We also worked on developing the novel structure determination technique, coherent diffraction imaging using X-ray free electron laser.
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