Metagenomic and metatranscriptomic analysis of rice paddy soil
| Project/Area Number |
25252013
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Senoo Keishi 東京大学, 大学院農学生命科学研究科(農学部), 教授 (40206652)
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| Co-Investigator(Kenkyū-buntansha) |
大塚 重人 東京大学, 農学生命科学研究科, 准教授 (10313074)
磯部 一夫 東京大学, 農学生命科学研究科, 助教 (30621833)
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| Co-Investigator(Renkei-kenkyūsha) |
HATTORI Masahira 早稲田大学, 先進理工学研究科, 教授 (70175537)
KUROKAWA Ken 国立遺伝学研究所, 生命情報研究センター, 教授 (20343246)
TAKAMI Hideto (独)海洋研究開発機構, 海洋・極限環境生物領域, チームリーダー (70359165)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥47,580,000 (Direct Cost: ¥36,600,000、Indirect Cost: ¥10,980,000)
Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2014: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2013: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
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| Keywords | 水田土壌 / メタゲノム解析 / メタトランスクリプトーム解析 / メタン生成・消去 / 窒素固定 / DNRA / 脱窒 / 脱窒反応 / DNRA反応 / 窒素固定反応 / デルタプロテオバクテリア / 鉄還元菌 / 土壌微生物 / 土壌学 |
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| Outline of Final Research Achievements |
Waterlogged paddy soil is characterized by surface oxidized and lower reduced layers, where various kind of oxidation/reduction reactions proceed. Shotgun DNA/RNA sequencing analysis (metagenomics and metatranscriptomics) of both layers soil samples revealed whole picture of microbial communities involved in methane generation and oxidation. Our analysis also revealed that most reductive nitrogen transformation (nitrogen fixation, DNRA and denitrification) gene transcripts were derived from Deltaproteobacteria, particularly the genera Anaeromyxobacter and Geobacter, previously known as iron reducers. This finding suggests the important role of these bacteria for nitrogen fertility of paddy soil.
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Report
(4 results)
Research Products
(15 results)
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[Presentation] 水田土壌の窒素循環に寄与する新規微生物群の発見2015
Author(s)
田伏 曜子, 角田 洋子, 伊藤 英臣, 白鳥 豊, 磯部 一夫, 大塚 重人, 妹尾 啓史
Organizer
日本微生物生態学会第30回大会 7th JTK Symposium
Place of Presentation
土浦亀城プラザ（茨城県土浦市）
Year and Date
2015-10-17
Related Report
Int'l Joint Research / Invited
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