secretome analysis of liver stage malaria parasies

• Project/Area Number: 25253027
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Mie University
• Principal Investigator: yuda masao 三重大学, 医学系研究科, 教授 (90293779)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000); Fiscal Year 2016: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000); Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000); Fiscal Year 2014: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000); Fiscal Year 2013: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
• Keywords: マラリア / 肝臓感染 / マラリア原虫 / 肝臓ステージ / ワクチン / トランスクリプトーム

Outline of Final Research Achievements

Liver stage malaria parasites establish environments suitable for their survival by manipulating the host hepatocyte. To understand molecular mechanisms of this control may lead to development of strategies to prevent malaria infection of humans. Parasite proteins exported to the host hepatocyte would have a central role in this control. In this study we performed mass sequencing analysis in this stage in order to identify whole proteins exported to the host hepatocyte (secretome).

Research Products

• [Journal Article] Activation and exhaustion of antigen-specific CD8+ T cells occur in different splenic compartments during infection with Plasmodium berghei. (2017)
  • Author(s): 1.Bayarsaikhan G, Miyakoda M, Yamamoto K, Kimura D, Akbari M, Yuda M, Yui K
  • Journal Title: Parasitol Int Volume: 66(3) Issue: 3 Pages: 227-235
  • DOI: 10.1016/j.parint.2017.01.022
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A potent adjuvant effect of a CD1d-binding NKT cell ligand in human immune system mice (2017)
  • Author(s): Li X, Huang J, Kaneko I, Zhang M, Iwanaga S, Yuda M, Tsuji M.
  • Journal Title: Expert Rev Vaccines. Volume: 16(1) Pages: 73-80
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A highly infectious Plasmodium yoelii parasite, bearing Plasmodium falciparum circumsporozoite protein (2016)
  • Author(s): Zhang M, Kaneko I, Tsao T, Mitchell R, Nardin EH, Iwanaga S, Yuda M, Tsuji M.
  • Journal Title: Malar J Volume: 201 Issue: 1 Pages: 15-15
  • DOI: 10.1186/s12936-016-1248-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Global transcriptional repression: An initial and essential step for Plasmodium sexual development (2015)
  • Author(s): Yuda M, Iwanaga S, Kaneko I, Kato T.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 112 Issue: 41 Pages: 12824-12829
  • DOI: 10.1073/pnas.1504389112
  • Peer Reviewed / Open Access
• [Journal Article] Genome-Wide Identification of the Target Genes of AP2-O, a Plasmodium AP2-Family Transcription Factor (2015)
  • Author(s): Yuda M, Iwanaga S, Kaneko I, Kato T
  • Journal Title: PLoS Pathog Volume: 11 Issue: 5 Pages: 1-27
  • DOI: 10.1371/journal.ppat.1004905
  • Peer Reviewed / Open Access
• [Journal Article] Olfactory plays a key role in spatiotemporal pathogenesis of cerebral malaria. (2014)
  • Author(s): H. Zhao, T. Aoshi, S. Kawai, Y. Mori, A. Konishi, M. Ozkan, Y. Fujita, Y. Haseda, M. Shimizu, M. Kohyama, K. Kobiyama, K. Eto, J. Nabekura, T. Horii, T. Ishino, M. Yuda, H. Hemmi, T. Kaisho, S. Akira, M. Kinoshita, K. Tohyama, Y. Yoshioka, K. J. Ishii, C. Coban
  • Journal Title: Cell Host Microbe Volume: 15 Issue: 5 Pages: 551-563
  • DOI: 10.1016/j.chom.2014.04.008
  • Peer Reviewed / Open Access
• [Journal Article] CD8+ T cells specific for a malaria cytoplasmic antigen form clusters around infected hepatocytes and are protective at the liver stage of infection. (2013)
  • Author(s): Kazumi Kimura, Daisuke Kimura, Yoshifumi Matsushima, Mana Miyakoda, Kiri Honma, Masao Yuda, and Katsuyuki Yui
  • Journal Title: Infection of Immunology Volume: 81 Issue: 10 Pages: 3825-3834
  • DOI: 10.1128/iai.00570-13
  • NAID: 120006986085
  • Peer Reviewed