Contamination status of antibiotics and antibiotic resistance genes (ARGs) in tropical Asian aquatic environments with artificial and natural disturbance

• Project/Area Number: 25257402
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Environmental impact assessment
• Research Institution: Ehime University
• Principal Investigator: Suzuki Satoru 愛媛大学, 沿岸環境科学研究センター, 教授 (90196816)
• Co-Investigator(Kenkyū-buntansha): 田村 豊 酪農学園大学, 獣医学部, 教授 (50382487) ; 高田 秀重 東京農工大学, (連合)農学研究科(研究院), 教授 (70187970)
• Research Collaborator: USUI Masaru 酪農学園大学, 獣医学群, 准教授 (60639540) ; Boonla Chanchai チュラロンコン大学, 生化学科, 教授 ; Sukpanyatham Nop Quality Vet Product,Inc., 社長
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000); Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2015: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000); Fiscal Year 2014: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000); Fiscal Year 2013: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
• Keywords: 抗生物質 / 薬剤耐性菌 / 薬剤汚染 / 水圏 / 熱帯アジア / 環境撹乱 / 抗生物質耐性菌

Outline of Final Research Achievements

Tropical Asian environments receive various origin effluents, which contain antibiotics and antibiotic resistance genes (ARGs). This study found that sulfonamides are abundant in veterinary and human discharges in Bangkok and rural areas. Cephalosporin resistant E. coli was highly abundant in animal effluent, and fly should be a vector of the bacteria. Yet-to-be cultured bacteria possess specific ARG of sulfonamide. This study could reveal specific characters of ARGs and antibiotic contamination in Thailand aquatic environments.

Research Products

• [Int'l Joint Research] Chulalongkorn University(タイ)
• [Int'l Joint Research] Chulalongkon University/Southern Vet. Res. Dev. Center(タイ)
• [Journal Article] Release and constancy of an antibiotic resistance gene in seawater under grazing stress by ciliates and heterotrophic nanoflagellates (2017)
  • Author(s): Bien TLT, Thao NV, Kitamura S-I, Obayashi Y, Suzuki S
  • Journal Title: Microbes and Environments Volume: 印刷中
  • NAID: 130006789877
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The novel mef(C)-mph(G) macrolide resistance gene cluster is conveyed in the environment on various vectors (2017)
  • Author(s): Sugimoto Y, Suzuki S, Nonaka LBoonla C, Sukpanyatham N Chou H-Y, Wu J-H
  • Journal Title: Journal of Global Antimicrobial Resistance Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Abundance of common aerobic anoxygenic phototrophic bacteria in a coastal aquaculture area (2016)
  • Author(s): Takabe Y-S, Nakao H, Kataoka T, Yokokawa T, Hamasaki K, Ohta K, Suzuki S
  • Journal Title: Front Microbiol Volume: 7
  • DOI: 10.3389/fmicb.2016.01996
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Use of Aeromonas spp. as general indicators of antimicrobial susceptibility among bacteria in aquatic environments in Thailand. (2016)
  • Author(s): Usui M, Tagaki C., Fukuda A, Okubo T, Boonla C, Suzuki S, Seki K, Takada H, Tamura Y.
  • Journal Title: Front Microbiol Volume: 7
  • DOI: 10.3389/fmicb.2016.00710
  • NAID: 120006666540
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Persistence of Multi-Drug Resistance Plasmids in Sterile Water under Very Low Concentrations of Tetracycline (2015)
  • Author(s): Thi Lan Thanh Bien, Yuki Sato-Takabe, Mitsuko Ogo, Masaru Usui, and Satoru Suzuki
  • Journal Title: Microbes and Environments Volume: 30 Issue: 4 Pages: 339-343
  • DOI: 10.1264/jsme2.ME15122
  • NAID: 130005116037
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Global occurrence of anti-infectives in contaminated surface waters: Impact of income inequality between countries (2015)
  • Author(s): Segura, PA, Takada, H, Correa, JA, Saadi, KE, Koike, T, O-Agiman, S, O-Anim, J, Sabi, EB, Wasonga, OV, Mgahalu, JM, dos Santos Jr AM, Newman B, Weerts, S and Yargeau, V.
  • Journal Title: Environmental International Volume: 80 Pages: 89-97
  • DOI: 10.1016/j.envint.2015.04.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sulfonamide and tetracycline resistance genes in total- and culturable-bacterial assemblages in South African aquatic environments (2015)
  • Author(s): Satoru Suzuki, Mitsuko Ogo, Tatsuya Koike, Hideshige Takada, and Brent Newman
  • Journal Title: Frontiers in Microbiology Volume: 6
  • DOI: 10.3389/fmicb.2015.00796
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Extracellular proteases are released by ciliates in defined seawater microcosms (2015)
  • Author(s): Thao NV, Nozawa A, Obayashi Y, Kitamura S-I, Yokokawa T, Suzuki S
  • Journal Title: Marine Environmental Research Volume: 109 Pages: 95-102
  • DOI: 10.1016/j.marenvres.2015.06.011
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel macrolide resistance genes, mef(C) and mph(G), carried by plasmids from Vibrio and Photobacterium isolated from sediment and seawater of a coastal aquaculture site. (2015)
  • Author(s): Lisa Nonaka, Fumito Maruyama, Satoru Suzuki, and Michiaki Masuda
  • Journal Title: Letters in Applied Microbiology Volume: 61 Pages: 1-6
  • Peer Reviewed / Open Access
• [Journal Article] Diversity of tetracycline resistant bacteria and resistance gene tet(M) in fecal microbial community of Adelie penguin in Antarctica (2015)
  • Author(s): Rahman, H.M., Sakamoto, K.Q., Kitamura, S-I., Nonaka, L. and Suzuki, S.
  • Journal Title: Polar Biology Volume: 38 Issue: 10 Pages: 1775-1778
  • DOI: 10.1007/s00300-015-1732-x
  • Peer Reviewed / Open Access
• [Journal Article] Spatial distribution and cell size of aerobic anoxygenic phototrophic bacteria in the Uwa Sea, Japan (2015)
  • Author(s): Sato-Takabe, Y., S. Suzuki, R. Shishikura, K. Hamasaki, Y. Tada, T. Kataoka, T. Yokokawa, N. Yoshie, S. Suzuki
  • Journal Title: J. Oceanogr. Volume: 71 Issue: 1 Pages: 151-159
  • DOI: 10.1007/s10872-014-0267-z
  • Peer Reviewed / Open Access
• [Journal Article] Tributyltin (TBT): a review on microbial resistance and degradation. (2015)
  • Author(s): Cruz, A., Anselmo, A.M., Suzuki, S. and Mendo, S.
  • Journal Title: Critical Reviews in Environmental Science and Technology Volume: 45 Issue: 9 Pages: 45-45
  • DOI: 10.1080/10643389.2014.924181
  • Peer Reviewed / Open Access
• [Journal Article] Novel macrolide resistance genes, mef(C) and mph(G), carried by plasmids from Vibrio and Photobacterium isolated from sediment and seawater of a coastal aquaculture site. (2015)
  • Author(s): Nonaka, L., Maruyama, F., Suzuki, S. and Masuda, M.
  • Journal Title: Letters in Applied Microbiology Volume: in press Issue: 1 Pages: 1-6
  • DOI: 10.1111/lam.12414
  • Peer Reviewed / Open Access
• [Journal Article] 水圏環境は薬剤耐性遺伝子のシンクかソースか？ (2015)
  • Author(s): 鈴木 聡
  • Journal Title: 動物用抗菌剤研究会誌 Volume: 印刷中
• [Journal Article] 日本の水環境中の抗生物質の分布と動態 (2015)
  • Author(s): 高田秀重
  • Journal Title: 動物用抗菌剤研究会誌 Volume: 印刷中
• [Journal Article] Various pAQU plasmids possibly contribute to disseminate tetracycline resistance gene tet(M) among marine bacterial community. (2014)
  • Author(s): Nonaka, L., Maruyama, F., Onishi, Y., Kobayashi, T., Ogura, Y., Hayashi, T., Suzuki, S. and Masuda, M.
  • Journal Title: Frontiers in Microbiology Volume: 5 Pages: 1-12
  • DOI: 10.3389/fmicb.2014.00152
  • Peer Reviewed / Open Access
• [Journal Article] Who possesses drug resistance genes in the aquatic environment? : sulfamethoxazole (SMX) resistance genes among the bacterial community in water environment of Metro-Manila, Philippines. (2013)
  • Author(s): Suzuki, S., Ogo, M., Miller, T.W., Shimizu, A., Takada, H. and Siringan, M.A.
  • Journal Title: Frontiers in Microbiology Volume: 4 Pages: 1-12
  • DOI: 10.3389/fmicb.2013.00102
  • Peer Reviewed
• [Journal Article] Management options for reducing release of antibiotics and antibiotic resistance genes to the environment. (2013)
  • Author(s): Pruden A, Larson, DGJ, Amezquita A, Collignon P, Brandt KK, Graham DW, Lazorchak JM, Suzuki S, Silly P, Snape JR, Topp E, Zhang T and Zhu Y-G
  • Journal Title: Environmental Health Perspectives Volume: 121 Issue: 8 Pages: 878-885
  • DOI: 10.1289/ehp.1206446
  • Peer Reviewed
• [Journal Article] 水溶性有機汚染物質による環境問題 (2013)
  • Author(s): 高田秀重
  • Journal Title: 水環境学会誌 Volume: 36 Pages: 308-313
  • NAID: 10031195331
  • Peer Reviewed
• [Presentation] 人獣医療と水圏環境間での薬剤耐性遺伝子の動態 (2017)
  • Author(s): 鈴木 聡
  • Organizer: 第32回日本環境感染学会総会・学術集会
  • Place of Presentation: 神戸国際展示場・神戸国際会議場・神戸ポートピアホテル（兵庫県・神戸市）
  • Year and Date: 2017-02-24
  • Invited
• [Presentation] 飢餓状態の菌および貧栄養環境での遺伝子水平伝達 (2016)
  • Author(s): 香山義明、鈴木 聡
  • Organizer: 日本微生物生態学会第31回大会
  • Place of Presentation: 横須賀市文化会館（神奈川県・横須賀市）
  • Year and Date: 2016-10-23
• [Presentation] 海洋細菌ゲノム上でマクロライド耐性遺伝子mef(C)-mph(G)を担うICEの解析 (2016)
  • Author(s): 杉本侑大、丸山史人、鈴木 聡
  • Organizer: 日本微生物生態学会第31回大会
  • Place of Presentation: 横須賀市文化会館（神奈川県・横須賀市）
  • Year and Date: 2016-10-23
• [Presentation] Formation and stability of extracellular antibiotic resistance plasmid pool in seawater under existence of ciliate and heterotrophic nanoflagella (2016)
  • Author(s): Thanh BTL, Thao NV, Kitamura S-I, Obayashi Y, Suzuki S.
  • Organizer: 日本微生物生態学会第31回大会
  • Place of Presentation: 横須賀市文化会館（神奈川県・横須賀市）
  • Year and Date: 2016-10-23
• [Presentation] Impact of humans on antibiotic resistance the aquatic environment. (2016)
  • Author(s): Suzuki S.
  • Organizer: 16th International Symposium on Microbial Ecology
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2016-08-20
  • Int'l Joint Research / Invited
• [Presentation] アジア・アフリカの下水汚染分子マーカーとしての合成甘味料の評価 (2016)
  • Author(s): 高田秀重、吉松賢治、磯貝早耶、関香奈子、小池達也
  • Organizer: 第50回水環境学会
  • Place of Presentation: アスティとくしま（徳島県・徳島）
  • Year and Date: 2016-03-16
• [Presentation] 新規マクロライド耐性遺伝子mef(C)およびmph(G)を担う遺伝因子とその伝達性 (2015)
  • Author(s): 杉本侑大、野中里佐、 鈴木 聡
  • Organizer: 第３０回日本微生物生態学会
  • Place of Presentation: 土浦亀城プラザ（茨城県・土浦）
  • Year and Date: 2015-10-20
• [Presentation] 水圏での薬剤耐性遺伝子保有者としてのyet-to-be cultured bacteria (2015)
  • Author(s): 鈴木 聡
  • Organizer: 第３０回日本微生物生態学会、日本細菌学会との連携シンポジウム２
  • Place of Presentation: 土浦亀城プラザ（茨城県・土浦）
  • Year and Date: 2015-10-19
  • Invited
• [Presentation] 動物から環境を介して伝播する細菌・耐性遺伝子 (2015)
  • Author(s): 臼井優
  • Organizer: 第11回札幌微生物勉強会
  • Place of Presentation: 北海道大学（北海道・札幌）
  • Year and Date: 2015-09-29
• [Presentation] タイの農場とヒトの生活環境での薬剤耐性菌及び薬剤耐性遺伝子伝播におけるハエの役割 (2015)
  • Author(s): 田垣智衣、臼井優、福田昭、高田秀重、鈴木聡、田村豊
  • Organizer: 第158回 日本獣医学会
  • Place of Presentation: 北里大学（青森県・十和田）
  • Year and Date: 2015-09-07
• [Presentation] 熱帯アジアの水圏環境に存在する薬剤耐性菌と残留抗菌薬濃度の関係 (2015)
  • Author(s): 福田昭、臼井優、田垣智衣、高田秀重、鈴木聡、田村豊
  • Organizer: 第158回 日本獣医学会
  • Place of Presentation: 北里大学（青森県・十和田）
  • Year and Date: 2015-09-07
• [Presentation] Antimicrobial-resistant bacteria and resistance genes from aquatic environment in Thailand, and a potential courier "fly" (2015)
  • Author(s): Usui M, Okubo T, Fukuda A, Boonla C, Takada H, Suzuki S, and Tamura Y.
  • Organizer: International Congress on Pathogens at the Human-Animal Interface (ICOPHAI)
  • Place of Presentation: Chiang Mai, Thailand
  • Year and Date: 2015-08-07
  • Int'l Joint Research
• [Presentation] 熱帯アジア・アフリカにおける抗生物質汚染の実態把握 (2015)
  • Author(s): 関香奈子、高田秀重、水川薫子、小池達也
  • Organizer: 第24回環境化学討論会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌）
  • Year and Date: 2015-06-25
• [Presentation] 水圏環境における抗菌薬汚染と薬剤耐性遺伝子の動態 (2015)
  • Author(s): 鈴木聡、高田秀重
  • Organizer: 第６３回日本化学療法学会シンポジウム
  • Place of Presentation: 京王プラザホテル（新宿区・東京）
  • Year and Date: 2015-06-04
  • Invited
• [Presentation] 耐性菌・耐性因子による環境汚染もしくは地球規模で広がる耐性菌汚染 (2015)
  • Author(s): 田村豊、渡辺治雄
  • Organizer: 第６３回日本化学療法学会シンポジウム
  • Place of Presentation: 京王プラザホテル（東京・新宿）
  • Year and Date: 2015-06-04
  • Invited
• [Presentation] 耐性遺伝子の環境拡散における野ネズミとハエの役割 (2015)
  • Author(s): 臼井優
  • Organizer: 第６３回日本化学療法学会シンポジウム
  • Place of Presentation: 京王プラザホテル（東京・新宿）
  • Year and Date: 2015-06-04
  • Invited
• [Presentation] Assessment of utility of artificial sweeteners as molecular markers to detect sewage contamination in Asian and African waters in comparison to traditional and emerging markers. (2015)
  • Author(s): Hideshige Takada, Kenji Yoshimatsu, Saya Isogai, Kanako Seki, Tatsuya Koike, and Akiko Shimizu
  • Organizer: 26th Annual meeting of Society of Environmental Toxicology and Chemistry Europe
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2015-05-04
  • Int'l Joint Research
• [Presentation] 養殖場環境における抗菌剤耐性遺伝子（ARGs）の分布：水圏でARGsを保有する菌はなにものか？ (2014)
  • Author(s): 鈴木 聡
  • Organizer: 抗感染薬開発フォーラム第９回セミナー
  • Place of Presentation: 慶応大学薬学部（東京都）
  • Year and Date: 2014-10-25
  • Invited
• [Presentation] 養殖場由来Vibrio sp.が保有する伝達性多剤耐性プラスミドの受容菌染色体への組み込み機構 (2014)
  • Author(s): 野中里佐，丸山史人，矢野大和，大西勇輝，廣瀬侑，鈴木聡，増田道明
  • Organizer: 環境微生物系合同大会2014
  • Place of Presentation: アクトシティ浜松（浜松市）
  • Year and Date: 2014-10-23
• [Presentation] バンコク周辺の水圏環境における薬剤耐性菌とその耐性遺伝子の解析 (2014)
  • Author(s): 大久保寅彦、臼井優、鈴木聡、高田秀重、田村豊
  • Organizer: 環境微生物系合同大会2014
  • Place of Presentation: アクトシティ浜松（浜松市）
  • Year and Date: 2014-10-23
• [Presentation] 水圏環境からの薬剤耐性遺伝子伝播におけるハエの役割 (2014)
  • Author(s): 臼井優、大久保寅彦、福田昭、高田秀重、鈴木聡、田村豊
  • Organizer: 環境微生物系合同大会2014
  • Place of Presentation: アクトシティ浜松（浜松市）
  • Year and Date: 2014-10-23
• [Presentation] The aquaculture environment: the focus on sulfonamide and tetracycline resistance genes. (2014)
  • Author(s): Satoru Suzuki
  • Organizer: 3rd International Conference on Responsible Use of Antibiotics in Animals
  • Place of Presentation: The Royal Tropical Institute, Amsterdam, The Netherland
  • Year and Date: 2014-09-29
  • Invited
• [Presentation] Who is possessing ARGs in freshwater and coastal sea? (2014)
  • Author(s): Satoru Suzuki
  • Organizer: 15th International Symposium on Microbial Ecology（Round table discussion, 鈴木聡・主催）
  • Place of Presentation: Coex Convention Center, Seoul, South Korea
  • Year and Date: 2014-08-25
  • Invited
• [Presentation] 水圏環境は薬剤耐性遺伝子のシンクかソースか？ (2014)
  • Author(s): 鈴木 聡
  • Organizer: 第41回動物用抗菌剤研究会シンポジウム
  • Place of Presentation: 日本獣医生命科学大学（武蔵野市）
  • Year and Date: 2014-04-26
  • Invited
• [Presentation] 水環境中の抗菌剤の分布と動態 (2014)
  • Author(s): 高田秀重
  • Organizer: 第41回動物用抗菌剤研究会シンポジウム
  • Place of Presentation: 日本獣医生命科学大学（武蔵野市）
  • Year and Date: 2014-04-26
  • Invited
• [Presentation] Diverse mechanisms of antibiotic resistance gene transfer in aquaculture. (2013)
  • Author(s): Suzuki, S. and Nonaka, L.
  • Organizer: The Canadian Society of Microbiologists 63rd Annual Conference, Ottawa
  • Place of Presentation: カナダ，オタワ
  • Invited
• [Presentation] 沿岸養殖場環境には多様な遺伝子水平伝達因子が混在する (2013)
  • Author(s): 大西勇輝，野中里佐，鈴木 聡
  • Organizer: 第２９回日本微生物生態学会大会
  • Place of Presentation: 鹿児島市
• [Presentation] 下水マーカーを用いたアフリカの水環境汚染の評価 (2013)
  • Author(s): 高田秀重，小池達也, Siaw Agyeman、John Ofosu-Anim, Edward Sabi、Oliver Wasonga、Brent Newman, Steven Weerts
  • Organizer: 第２２回環境化学討論会
  • Place of Presentation: 府中市
• [Book] Antibiotic Resistance in Aquatic Systems (2017)
  • Author(s): Suzuki S, Pruden A, Virta M, Zhang T. (Eds)
  • Total Pages: 107
  • Publisher: Frontiers Media
• [Book] 3rd Edition, Foodborne Diseases (2017)
  • Author(s): Wondwossen AG, Thomas W, Gregory H, Walid A, Usui M, Suzuki S.
  • Publisher: Elsevier
• [Book] Antibiotic Resistance Genes in Natural Environments and Long-Term Effect (2017)
  • Author(s): Suzuki S.
  • Total Pages: 190
  • Publisher: Nova Science Publishers, Inc.
• [Book] 水圏微生物学の基礎 (2015)
  • Author(s): 鈴木 聡（第７、１３章を執筆）
  • Total Pages: 261
  • Publisher: 恒星社厚生閣
• [Remarks] 愛媛大学沿岸環境科学研究センター生態系解析部門（海洋分子生態学）
  • URL: http://web.agr.ehime-u.ac.jp/~mme/
• [Funded Workshop] Contamination with antibiotics and antibiotic resistant bacteria in Thai aquatic systems (2016)
  • Place of Presentation: 愛媛大学（愛媛県・松山市）