Single cell biomechanics and the investigation of mechanical field in cell nucleus

Research Project

Project/Area Number	25282133
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Partial Multi-year Fund
Section	一般
Research Field	Biomedical engineering/Biomaterial science and engineering
Research Institution	Aino University (2016) Osaka University (2013-2015)
Principal Investigator	MIYAZAKI Hiroshi 藍野大学, 医療保健学部, 教授 (00263228)
Project Period (FY)	2013-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2013: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Keywords	細胞 / 細胞核 / ストレスファイバー / 力学的特性 / 力学場 / 形態
Outline of Final Research Achievements	In the micropipette aspiration test of a single cell, elastic modulus of isolated nucleus was about 4 times higher than that of a whole cell. While, in the compressive test of a single cell, elastic modulus of isolated nucleus was about 2 times higher than that of a whole cell. From the averaged elastic modulus of the nuclei of fibroblasts and change in averaged height of nuclei in adherent fibroblasts before and after F-actin/stress fiber disruption, compressive stress imposed on nuclei by stress fibers in adherent fibroblasts was estimated to be about 0.8 kPa.