Development of electrochemical detection of food poisoning bacteria based on molecular imprinting technique

• Project/Area Number: 25288039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Functional solid state chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: SHIIGI Hiroshi 大阪府立大学, 工学(系)研究科(研究院), 准教授 (70335769)
• Co-Investigator(Renkei-kenkyūsha): NAGAOKA TSUTOMU 大阪府立大学, 工学研究科, 教授 (00172510) ; TOKONAMI SHIHO 大阪府立大学, 工学研究科, 准教授 (60535491) ; NISHINO TOMOAKI 東京工業大学, 大学院理学研究科, 准教授 (80372415)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 分子鋳型 / バイオ分析 / 表面分析 / バイオセンサ / 大腸菌 / 細胞鋳型 / ナノ粒子 / 分子探針

Outline of Final Research Achievements

Pathogenic bacteria in food products have caused serious problems on public health. A rapid bacterial detection method has been developed through the formation of an artificial receptor without the biological function of bacteria.The artificial receptor formed on the polymer film can identify different types of E.coli based on the O-antigen. Moreover, we have succeeded in the visualization of bacteria using gold nanoparticle-labels with the molecular modification and developed a simple detection method focusing on the electrochemical properties of bacteria.

Research Products

• [Journal Article] Light-scattering Characteristics of Metal Nanoparticles on a Single Bacterial Cell (2016)
  • Author(s): T. Kinoshita, K. Kiso, D. Q. Le, H. Shiigi, T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 32 Issue: 3 Pages: 301-305
  • DOI: 10.2116/analsci.32.301
  • NAID: 130005131634
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nanoantennas as biomarkers for bacterial detection (2015)
  • Author(s): H. Shiigi, T. Kinoshita, M. Fukuda, D. Q. Le, T. Nishino, and T. Nagaoka
  • Journal Title: Anal. Chem. Volume: 87 Issue: 7 Pages: 4042-4046
  • DOI: 10.1021/acs.analchem.5b00415
  • Peer Reviewed
• [Journal Article] Development of an Observation Platform for Bacterial Activity Using Polypyrrole Films Doped with Bacteria (2015)
  • Author(s): L.Q. Dung, M. Takai, S. Suekuni, S. Tokonami, T. Nishino, H. Shiigi, T. Nagaoka
  • Journal Title: Analytical Chemistry Volume: 87 Issue: 7 Pages: 4047-4052
  • DOI: 10.1021/acs.analchem.5b00544
  • Peer Reviewed
• [Journal Article] Electrochemical Evaluation of Poly(3,4-ethylenedioxythiophene) Films Doped with Bacteria Based on Viability Analysis (2015)
  • Author(s): D. Q. Le, S. Tokonami, T. Nishino H. Shiigi, T. Nagaoka*
  • Journal Title: Bioelectrochem Volume: 105 Pages: 50-55
  • DOI: 10.1016/j.bioelechem.2015.05.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Voltammetric Detection and Profiling of Isoprenoid Quinones Hydrophobically Transferred From Bacterial Cells (2015)
  • Author(s): D. Q. Le, A. Morishita, S. Tokonami, T. Nishino, H. Shiigi, M. Miyake, T. Nagaoka
  • Journal Title: Anal. Chem. Volume: 87(16) Issue: 16 Pages: 8416-8423
  • DOI: 10.1021/acs.analchem.5b01772
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 光アンテナの形成による病原性細菌の迅速検出 (2015)
  • Author(s): 木下隆将, 椎木 弘, 長岡 勉
  • Journal Title: 化学工学 Volume: 66 Pages: 812-816
• [Journal Article] One-by-one single-molecule detection of mutated nucleobases by monitoring tunneling current using a DNA tip (2015)
  • Author(s): P. T. Bui, T. Nishino, H. Shiigi, T. Nagaoka
  • Journal Title: Chem. Commun., Volume: 51 Issue: 9 Pages: 1666-1669
  • DOI: 10.1039/c4cc08227c
  • Peer Reviewed
• [Journal Article] Efficient Collection and Sensitive Detection using Conducting Magnetic Microbeads (2014)
  • Author(s): H. Shiigi, T. Kinoshita, N. Shibutani, T. Nishino, T. Nagaoka
  • Journal Title: Anal. Chem. Volume: 86 Issue: 10 Pages: 4977-4981
  • DOI: 10.1021/ac500452w
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Construction of nanoantennas on the bacterial outer membrane (2014)
  • Author(s): H. Shiigi, M. Fukuda, T. Tono, K. Takada, T. Okada, L. Q. Dung, Y. Hatsuoka, T. Kinoshita, M. Takai, S. Tokonami, H. Nakao, T. Nishino, Y. Yamamoto. T. Nagaoka
  • Journal Title: Chemical Communications Volume: 50 Issue: 47 Pages: 6252-6255
  • DOI: 10.1039/c4cc01204f
  • Peer Reviewed
• [Journal Article] Fabrication of Bacteria-Imprinted-Polymer Based Sensors Using Over Oxidized Polypyrrole (2014)
  • Author(s): T. Nagaoka, H. Shiigi, T. Nishino, S. Tokonami
  • Journal Title: Chemical Sensors Volume: 30 Pages: 22-24
  • NAID: 40020064577
• [Journal Article] Molecularly Bridged Gold Nanoparticle Array for Sensing Applications (2014)
  • Author(s): H. Shiigi, T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 30 Issue: 1 Pages: 89-96
  • DOI: 10.2116/analsci.30.89
  • NAID: 130004442251
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Tracking the Growth of Tadpole-shaped Aggregates by Scanning Electron Microscopy (2014)
  • Author(s): T. Kinoshita, H. Murakami, Y. Muranaka, Y. Yamamoto, T. Nishino, H. Shiigi, and T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 30 Issue: 3 Pages: 319-322
  • DOI: 10.2116/analsci.30.319
  • NAID: 130004827414
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Quantitative Exploration of Electron Transfer in a Single Noncovalent Supramolecular Assembly (2013)
  • Author(s): P.T. Bui, T. Nishino, Y. Yamamoto, H. Shiigi
  • Journal Title: J. Am. Chem. Soc. Volume: 135 Issue: 14 Pages: 5238-5241
  • DOI: 10.1021/ja312019p
  • Peer Reviewed
• [Journal Article] Label-Free and Selective Bacteria Detection Using a Film with Transferred Bacterial Configuration (2013)
  • Author(s): S. Tokonami, Y. Nakadoi, K. Saimatsu, M. Takahashi, M. Ikemizu, T. Kadoma, L. Q. Dung, H. Shiigi, T. Nagaoka
  • Journal Title: Analytical Chemistry Volume: 85 Issue: 10 Pages: 4925-4929
  • DOI: 10.1021/ac3034618
  • Peer Reviewed
• [Journal Article] 3．金ナノ粒子2次元構造体の作製と電気的センシングへの応用 (2013)
  • Author(s): 椎木 弘，長岡 勉
  • Journal Title: Electrochemistry Volume: 81 Issue: 8 Pages: 646-649
  • DOI: 10.5796/electrochemistry.81.646
  • NAID: 10031178983
  • ISSN: 1344-3542, 2186-2451
  • Peer Reviewed
• [Journal Article] 分子鋳型を用いる化学センサの開発と応用 (2013)
  • Author(s): 椎木 弘, 床波志保, 長岡 勉
  • Journal Title: 化学工学 Volume: 64(4) Pages: 27-32
• [Journal Article] Electrical Properties of Two-Dimensional Gold Nanoparticle-Alkanethiol Networks Formed on Plastic Microbeads (2013)
  • Author(s): H. Shiigi, S. Shirai, T. Fujita, H. Morishita, Y. Yamamoto, T. Nishino, S. Tokonami, H. Nakao, T. Nagaoka
  • Journal Title: J. Electrochem. Soc. Volume: 160（9） Issue: 9 Pages: H630-H635
  • DOI: 10.1149/2.110309jes
  • Peer Reviewed
• [Journal Article] Simple Preparation of Microstructure by Using an Organic–Inorganic Raspberry-like Hybrid as a Building Block (2013)
  • Author(s): H. Shiigi, Y. Muranaka, Y. Yamamoto, T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 29 Issue: 10 Pages: 937-939
  • DOI: 10.2116/analsci.29.937
  • NAID: 10031202852
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Electrochemical Catalytic Activity of Organic-Inorganic Hybrid Nanoraspberry Consisted of Gold Nanoparticle and Aniline Oligomer, (2013)
  • Author(s): H. Shiigi, Y. Muranaka, Y. Hatsuoka, Y. Yamamoto, T. Nagaoka
  • Journal Title: J. Electrochem. Soc. Volume: 160（10） Issue: 11 Pages: H813-H817
  • DOI: 10.1149/2.058311jes
  • Peer Reviewed
• [Presentation] Conducting polymers for bacterial detection :Applications to sensors and trapping agents (2015)
  • Author(s): L. Q. Dung, T. Kinoshita, A. Morishita, S. Tokonami, T. Nishino, H. Shiigi, T. Nagaoka
  • Organizer: Pacifichem 2015
  • Place of Presentation: ハワイコンベンションセンター，ホノルル，米国
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Development of an observation platform for bacterial activity using conducting polymer films doped with bacteria (2015)
  • Author(s): S. Suekuni, T. Tamura, H. Shiigi, T. Nagaoka
  • Organizer: Pacifichem 2015
  • Place of Presentation: ハワイコンベンションセンター，ホノルル，米国
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Electrochemical evaluation of bacterial viability using fluorescent labels (2015)
  • Author(s): M. Takai, K. Ishiki, H. Shiigi, T. Nagaoka
  • Organizer: Pacifichem 2015
  • Place of Presentation: ハワイコンベンションセンター，ホノルル，米国
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 機能的ナノ空間の創成と分子認識への応用 (2015)
  • Author(s): 椎木弘
  • Organizer: 第61回ポーラログラフィおよび電気分析化学討論会
  • Place of Presentation: イーグレ姫路，姫路，兵庫
  • Year and Date: 2015-11-25
  • Invited
• [Presentation] Voltammetric sensing and profiling of isoprenoid quinones hydrophoobically transferred from bacterial cells deposited on an ITO electrode (2015)
  • Author(s): L. Q. Dung, A. Morishita, S. Tokonami, T. Nishino, H. Shiigi, T. Nagaoka
  • Organizer: Asian Conference on Chemical Sensor
  • Place of Presentation: シャングリラリゾート，ペナン，マレーシア
  • Year and Date: 2015-11-16
  • Int'l Joint Research
• [Presentation] Development of Bio-Platform Using Conducting Polymer Films for Bacterial Activity (2015)
  • Author(s): S. Suekuni, M. Takai, T. Tamura, L. Q. Dung, S. Tokonami, H. Shiigi, T. Nagaoka
  • Organizer: Asian Conference on Chemical Sensor
  • Place of Presentation: シャングリラリゾート，ペナン，マレーシア
  • Year and Date: 2015-11-16
  • Int'l Joint Research
• [Presentation] Development of Conducting Polymer-based Platforms for Monitoring Bacterial Activity (2015)
  • Author(s): L. Q. Dung, M. Takai, S. Suekuni, S. Tokonami, H. Shiigi, T. Nagaoka
  • Organizer: 7th East Symposium on Functional Dyes and Advanced Materials
  • Place of Presentation: 大阪府立大学，堺，大阪
  • Year and Date: 2015-09-02
  • Int'l Joint Research
• [Presentation] 電解による微生物固定化電極の作製と活性の評価 (2015)
  • Author(s): 陶国智史, 高井将博, L. D. Quynh, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 電気化学会第82回大会
  • Place of Presentation: 横浜国立大学（神奈川県横浜市）
  • Year and Date: 2015-03-15 – 2015-03-17
• [Presentation] 導電性高分子を用いたバイオテンプレートの作製と評価 (2015)
  • Author(s): 陶国智史, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: Bio Medical Forum 2015
  • Place of Presentation: 大阪府立大学（大阪府堺市）
  • Year and Date: 2015-01-23
• [Presentation] Fabricated with Conducting Polymers Doped with Template Bacteria (2014)
  • Author(s): T. Nagaoka, S. Tokonami, T. Nishno, H. Shiigi, Q. Dung
  • Organizer: 1st E-MRS/MRS-J Bilateral Symposia
  • Place of Presentation: 横浜情報文化センター（神奈川県横浜市）
  • Year and Date: 2014-12-10 – 2014-12-14
• [Presentation] Electrochemical Characteristics of Poly(3,4-Ethylenedioxythiophene) Doped with Bacteria (2014)
  • Author(s): Dung Le Quynh, T. Nishino, S. Tokonami, H. Shiigi, T. Nagaoka
  • Organizer: ICFIA19
  • Place of Presentation: アクロス福岡（福岡県福岡市）
  • Year and Date: 2014-11-30 – 2014-12-05
• [Presentation] 隙間の化学 (2014)
  • Author(s): 椎木 弘, 西野智昭, 床波志保, 長岡 勉
  • Organizer: 第60回ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 京都工芸繊維大学（京都府京都市）
  • Year and Date: 2014-11-15 – 2014-11-16
  • Invited
• [Presentation] 細菌を吸着させた電極のボルタンメトリ (2014)
  • Author(s): 椎木 弘, 西野智昭, 床波志保, 長岡 勉
  • Organizer: 第60回ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 京都工芸繊維大学（京都府京都市）
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] Direct Single-molecule Detection of DNA through Electron Transfer Induced by Hybridization (2014)
  • Author(s): T. Nishino, P. T. Bui, H. Shiigi, T. Nagaoka
  • Organizer: ISSS-7
  • Place of Presentation: くにびきメッセ（島根県松江市）
  • Year and Date: 2014-11-02 – 2014-11-06
• [Presentation] 電解による微生物の固定化と生存率の評価 (2014)
  • Author(s): 陶国智史, 高井将博, L. D. Quynh, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 2014年電気化学秋季大会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] バクテリア内での蛍光標識の電気化学的挙動 (2014)
  • Author(s): 初岡 優, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 2014年電気化学秋季大会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] 導電性高分子を用いたバイオプラットフォームの開発 (2014)
  • Author(s): 高井将博，陶国智史，西野智昭，椎木 弘，長岡 勉
  • Organizer: 第75回応用物理学会秋季学術講演会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2014-09-17 – 2014-09-20
• [Presentation] 細菌の導電性ポリマへの固定化と化学センサへの応用 (2014)
  • Author(s): 長岡 勉, 椎木 弘, 西野智昭, 床波志保
  • Organizer: 日本分析化学会第63年会
  • Place of Presentation: 広島大学（広島県東広島市）
  • Year and Date: 2014-09-17 – 2014-09-19
  • Invited
• [Presentation] 細菌を組み込んだ導電性高分子膜電極の作製と細菌センサへの応用 (2014)
  • Author(s): 長岡 勉, 椎木 弘, 西野智昭, 床波志保
  • Organizer: 2014年電気化学秋季大会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2014-09-17 – 2014-09-19
  • Invited
• [Presentation] 導電性高分子を用いたバイオテンプレートの作製 (2014)
  • Author(s): 陶国智史, 高井将博, L. D. Quynh, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 日本分析化学会第63年会
  • Place of Presentation: 広島大学（広島県東広島市）
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] 導電性高分子膜の微生物親和性の評価 (2014)
  • Author(s): 陶国智史, 高井将博, L. D. Quynh, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 日本分析化学会第63年会
  • Place of Presentation: 広島大学（広島県東広島市）
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] Bacteria-Imprinted Conducting-Polymers for Applications to Bacteria Monitoring Platforms and Sensors (2014)
  • Author(s): L. Q. Dung, H. Shiigi, T. Nishino, S. Tokonami, T. Nagaoka
  • Organizer: XIII European Vacuum Conference
  • Place of Presentation: Aveiro (Portugal)
  • Year and Date: 2014-09-08 – 2014-09-12
• [Presentation] Exploring Electron Tranfer through Noncovalent Interactions between Single Molecules (2014)
  • Author(s): T. Nishino, H. Shiigi, T. Nagaoka
  • Organizer: XIII European Vacuum Conference
  • Place of Presentation: Aveiro (Portugal)
  • Year and Date: 2014-09-08 – 2014-09-12
• [Presentation] Electrical Properties of Two-Dimensional Gold Nanoparticle Array on a Microbead (2014)
  • Author(s): H. Shiigi, Y. Takai, T. Nishino, Y. Yamamoto, T. Nagaoka
  • Organizer: ECOSS30
  • Place of Presentation: Antalya (Turkey)
  • Year and Date: 2014-08-31 – 2014-09-05
• [Presentation] 過酸化ポリピロール膜を用いる細菌鋳型センサの作製 (2014)
  • Author(s): 長岡 勉, 椎木 弘, 西野智昭, 床波志保
  • Organizer: 第56回化学センサ研究発表会
  • Place of Presentation: 大阪
  • Invited
• [Presentation] Electrochemical characteristics of biofiim composed of poly(3,4-ethylenedioxythiophene) and bacteria (2014)
  • Author(s): L. Q. Dung, 初岡 優, 椎木 弘, 長岡 勉
  • Organizer: 電気化学会第81回大会
  • Place of Presentation: 大阪
• [Presentation] バイオセンシングのための導電性高分子テンプレートの作製 (2014)
  • Author(s): 陶国智史, L. Q. Dung, 細末健太, 椎木 弘, 長岡 勉
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋
• [Presentation] 導電性高分子テンプレートを用いたバイオ分析法の開発 (2014)
  • Author(s): 高井将博, L. Q. Dung, 椎木 弘, 長岡 勉
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋
• [Presentation] 金属ナノ粒子標識によるバクテリアの検出 (2014)
  • Author(s): 福田真帆, 初岡 優, 木下隆将, L. Q. Dung, 椎木 弘, 長岡 勉
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋
• [Presentation] Bacteria-imprinted conducting-polymer based sensors/detectors (2013)
  • Author(s): L. Q. Dung, H. Shiigi, S. Tokonami, T. Nagaoka
  • Organizer: 18th ICFIA
  • Place of Presentation: Porto, Portugal
• [Presentation] Bacteria Detection Using a Film with Transferred Bacterial Configuration (2013)
  • Author(s): S. Tokonami, H. Nakata, T. Kadoma, S. Takami, H. Shiigi, T. Nagaoka
  • Organizer: 8th ICFIA
  • Place of Presentation: Porto, Portugal
• [Presentation] Development of Electrochemical Biodetection System Using Magnetic Conducting Microbeads (2013)
  • Author(s): M. Kinoshita, N. Shibutani,Y. Yamamoto, H. Shiigi, T. Nagaoka,
  • Organizer: RSC Tokyo International Conference
  • Place of Presentation: Makuhari
• [Presentation] Electrochemical Analysis of Bacteria Using Fluorescent Labels (2013)
  • Author(s): Y. Hatsuoka, L. Q. Dung, H. Shiigi, T. Nagaoka
  • Organizer: RSC Tokyo International Conference
  • Place of Presentation: Makuhari
• [Presentation] Quantification of Electron Transfer through a Single Supramolecule by Fullerene Molecular STM Tip (2013)
  • Author(s): T. Nishino, P. Tan Bui, Y. Yamamoto, H. Shiigi
  • Organizer: ACSIN-12&ICSPM21
  • Place of Presentation: Tsukuba
• [Presentation] Surface Analysis of Bacteria Using Gold Nanoparticle (2013)
  • Author(s): T. Okada, K. Takada, M. Fukuda, T. Tono, L. Q. Dung, S. Tokonami, T. Nishino, H. Shiigi, T. Nagaoka
  • Organizer: AsiananalysisVII
  • Place of Presentation: Fukuoka
• [Presentation] Immobilization of Living Bacteria into Polypyrrole Film (2013)
  • Author(s): K. Saimatsu, S. Suekuni, L. Q. Dung, S. Tokonami, H. Shiigi, T .Nagaoka
  • Organizer: AsiananalysisVII
  • Place of Presentation: Fukuoka
• [Presentation] Immobilization of Bacteria into Poly(3,4-ethylenedioxythiophene) (PEDOT) Film (2013)
  • Author(s): L. Q. Dung, K. Saimatsu,Y. Hatsuoka, S. Tokonami, H. Shiigi, T. Nagaoka
  • Organizer: AsiananalysisVII
  • Place of Presentation: Fukuoka
• [Presentation] 蛍光標識化バクテリアの電気化学的評価及び検出 (2013)
  • Author(s): 初岡 優, L. Q. Dung, 椎木 弘, 長岡 勉
  • Organizer: 第59回ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 石垣
• [Presentation] ナノ粒子を用いたバクテリア表面の構造解析 (2013)
  • Author(s): 岡田知之, 福田真帆, 東野貴俊, 高井将博, 椎木 弘, 長岡 勉
  • Organizer: 2013年電気化学秋季大会
  • Place of Presentation: 東京
• [Presentation] Immobilization of Bacteria into Poly(3,4-ethylenedioxythiophene) (PEDOT) Film Modified Electrode (2013)
  • Author(s): L. Q. Dung, 床波志保, 椎木 弘, 長岡 勉
  • Organizer: 2013年電気化学秋季大会
  • Place of Presentation: 東京
• [Presentation] 蛍光標識の電気化学特性に基づいたバクテリアの構造解析 (2013)
  • Author(s): 初岡 優, L. Q. Dung, 椎木 弘, 長岡 勉
  • Organizer: 2013年電気化学秋季大会
  • Place of Presentation: 東京
• [Presentation] ポリピロールに固定化したバクテリアの呼吸活性の電気化学的評価 (2013)
  • Author(s): 細末健太, L. Q. Dung, 陶国智史, 床波志保, 椎木 弘, 長岡 勉
  • Organizer: 2013年電気化学秋季大会
  • Place of Presentation: 東京
• [Presentation] 蛍光標識によるバクテリアの電気化学検出法の開発 (2013)
  • Author(s): 初岡 優, L. Q. Dung, 椎木 弘, 長岡 勉
  • Organizer: 日本分析化学第62年会
  • Place of Presentation: 東大阪
• [Presentation] 金ナノ粒子を用いたバクテリア表面の化学構造解析 (2013)
  • Author(s): 岡田知之, 福田真帆, 東野貴俊, L. Q. Dung, 床波志保, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 日本分析化学第62年会
  • Place of Presentation: 東大阪
• [Presentation] 細菌形状を転写した高分子膜による細菌検出と実分析への展開 (2013)
  • Author(s): 中田啓之, 高見星司, 門真哲也, 椎木 弘, 長岡 勉, 床波志保
  • Organizer: 日本分析化学第62年会
  • Place of Presentation: 東大阪
• [Presentation] Immobilization of Various Bacteria into Poly(3,4-ethylenedioxythiophene) (PEDOT) Film (2013)
  • Author(s): L. Q. Dung, 床波志保, 椎木 弘, 長岡 勉
  • Organizer: 日本分析化学第62年会
  • Place of Presentation: 東大阪
• [Presentation] 菌体複合ポリピロール膜によるバイオテンプレートの開発 (2013)
  • Author(s): 細末健太, L. Q. Dung, 陶国智史, 床波志保, 椎木 弘, 長岡 勉
  • Organizer: 日本分析化学第62年会
  • Place of Presentation: 東大阪
• [Presentation] 導電性高分子を利用した細菌検出 (2013)
  • Author(s): 中田啓之,中土井祐, 高橋茉里, 池水麦平, 門真哲也, 椎木 弘, 長岡 勉, 床波志保
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 函館
• [Presentation] 蛍光標識の電気化学特性に基づいたバクテリア検出法の開発 (2013)
  • Author(s): 初岡 優, 細末健太, L. Q. Dung, 岡田知之, 椎木 弘, 長岡 勉
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 函館
• [Presentation] バクテリアの標識化による電気化学検出法の開発 (2013)
  • Author(s): 初岡 優, 細末健太, 岡田知之, L. Q. Dung, 椎木 弘, 長岡 勉
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 函館
• [Presentation] Immobilization of bacteria into conducting polymer films and applications to imprinted-polymer based sensing (2013)
  • Author(s): L. Q. Dung, K. Saimatsu, S. Tokonami, H. Shiigi, T. Nagaoka
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 函館
• [Presentation] Electrochemical Devices using Conducting polymer films doped with bacteria
  • Author(s): L. Q. Dung, 細末健太, 床波志保, 椎木 弘, 長岡 勉
  • Organizer: 第51回フローインジェクション分析講演会
  • Place of Presentation: 熊本
• [Book] 基礎分析化学 (2015)
  • Author(s): 椎木 弘, 長岡 勉
  • Total Pages: 198
  • Publisher: 朝倉書店
• [Book] 基礎分析化学（第9章 生物学的分析） (2015)
  • Author(s): 椎木 弘, 長岡 勉
  • Total Pages: 208
  • Publisher: 朝倉書店
• [Book] 製品中に含まれる（超）微量成分・不純物の同定・定量ノウハウ (2014)
  • Author(s): 床波志保, 中田啓之, 椎木 弘, 長岡 勉
  • Total Pages: 818
  • Publisher: 技術情報協会
• [Book] バイオセンサの先端科学技術と新製品への応用開発 (2014)
  • Author(s): 椎木 弘, 床波志保, 陳 智棟, 長岡 勉
  • Total Pages: 534
  • Publisher: 技術情報協会
• [Book] 導電性ポリマー材の高機能化と用途開発最前線 (2014)
  • Author(s): 長岡 勉, 椎木 弘，床波志保
  • Total Pages: 286
  • Publisher: エヌ・ティ－・エス
• [Book] プラズモンナノ材料開発の最前線と応用 (2013)
  • Author(s): 長岡 勉, 椎木 弘
  • Total Pages: 280
  • Publisher: CMC出版
• [Book] 金属ナノ・マイクロ粒子の最新技術と応用 (2013)
  • Author(s): 床波志保, 椎木 弘, 長岡 勉
  • Total Pages: 236
  • Publisher: CMC出版
• [Remarks] 大阪府立大学分子認識化学研究グループHP
  • URL: http://www.chem.osakafu-u.ac.jp/ohka/ohka12/index.html
• [Remarks] 大阪府立大学教員活動情報
  • URL: http://kyoindb.osakafu-u.ac.jp/html/100599_ja.html
• [Remarks] 大阪府立大学教員活動情報
  • URL: http://kyoindb.osakafu-u.ac.jp/html/100599_ja.html?k=%E6%A4%8E%E6%9C%A8%E5%BC%98
• [Remarks] 椎木 弘 - 教員活動情報データベース ｜大阪府立大学
  • URL: http://kyoindb.acs.osakafu-u.ac.jp/profile/out.IO76cF4.PmpexVOboXnfZQ==.html