| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
This research project developed sensor films for real-time microbial detection using the imprinting technique that was developed by our group. The sensor films were prepared from polypyrrole film doped with a targeted microbe, such as E. coli O157. The microbe was then removed by over-oxidation of the film, which created the cavities complimentary to the template microbe. In this research project, we focused on preparing highly selective sensor films by optimizing polymerization parameters. In addition, highly sensitive electrochemical detection technique was developed using a tetrazolium salt. These efforts were integrated to realize the fabrication of real-time microbial sensors.
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