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High density cultivation of anaerobic protists in UASB reactors fed with domestic sewage

Research Project

Project/Area Number 25289174
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Civil and environmental engineering
Research InstitutionNagaoka National College of Technology

Principal Investigator

ARAKI Nobuo  長岡工業高等専門学校, 環境都市工学科, 教授 (30193072)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Takashi  長岡技術科学大学, 工学部, 教授 (10280447)
OSHIKI Mamoru  長岡工業高等専門学校, 環境都市工学科, 助教 (90540865)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Keywords都市下水処理 / 嫌気性処理 / メタン生成 / 原生動物 / 共生微生物 / 嫌気性処理法
Outline of Final Research Achievements

This study aimed to investigate influences of anaerobic protozoa on treatment efficiencies of UASB reactor including COD removal, methane production and sludge volume and on bacterial and archaeal community structures in anaerobic sludge. Two UASB reactors fed with domestic sewage were operated for 171 days at COD loading rates of 0.5 to 4 gCOD L-1 day-1, and protozoa in one UASB reactor was killed by addition of cycloheximide. Methane production by the sludge with protozoa was higher than the sludge without protozoa. Phylogetic diversity was higher in the sludge with protozoa, where syntrophic bacteria affiliated with the class Deltaproteobacteria were more abundant. And this present study illuminates prokaryotic community structure in Metopus ciliates, identifying M. boonei- and C. aminobutyricum-like prokaryotes as endosymbionts.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (21 results)

All 2015 2014 2013

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge2015

    • Author(s)
      Yuga Hirakata, Mamoru Oshiki, Kyohei Kuroda, Masashi Hatamoto, Kengo Kubota, Takashi Yamaguchi, Hideki Harada and Nobuo Araki
    • Journal Title

      Microbes & Environments

      Volume: 30 Pages: 335-338

    • NAID

      130005116034

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 下水処理UASB後段のDHSリアクターにおける排水処理特性と真核生物群集構造の季節変動2013

    • Author(s)
      宮岡佑馬・幡本将史,珠坪一晃・小野寺崇,多川正,上村繁樹,荒木信夫,山口隆司
    • Journal Title

      土木学会論文集G (環境)

      Volume: 69 Pages: 257-263

    • NAID

      130004962545

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 嫌気性原生動物の体内に生息する共生バクテリア・アーキアの分子生物学的解析2015

    • Author(s)
      平片悠河, 押木守, 荒木信夫, 幡本将史, 山口隆司
    • Organizer
      第33回土木学会新潟会調査研究発表会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 都市下水処理UASB槽の処理性能および微生物叢に及ぼす嫌気性原生動物の影響2015

    • Author(s)
      平片悠河, 押木守, 黒田恭平, 幡本将史, 荒木信夫, 山口隆司
    • Organizer
      第18回水環境シンポジウム
    • Place of Presentation
      信州大学(長野県長野市)
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] Impacts of protist bacterivory by anaerobic ciliates on prokaryotic community function and structure in anaerobic granular sludge of up-flow anaerobic sludge blanket (UASB) reactor2015

    • Author(s)
      Yuga Hirakata, Mamoru Oshiki, Kyohei Kuroda, Masashi Hatamoto, Kengo Kubot a, Takashi Yamaguchi, Hideki Harada And Nobuo Araki
    • Organizer
      Water and Environment Technology (WET) Conference 2015
    • Place of Presentation
      日本大学(東京都千代田区)
    • Year and Date
      2015-08-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] INFLUENCE OF ANAEROBIC PROTOZOA ON TREATMENT EFFICIENCY AND BACTERIAL AND ARCHAEAL COMMUNITY STRUCTURES IN UASB REACTORS FED WITH DOMESTIC SEWAGE2015

    • Author(s)
      Yuga Hirakata, Kyohei Kuroda, Mamoru Oshiki, Nobuo Araki, Masashi Hatamoto, Takashi Yamaguchi
    • Organizer
      The 4th International GIGAKU Conference in Nagaoka
    • Place of Presentation
      長岡技科大(新潟県長岡市)
    • Year and Date
      2015-06-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 培地の酸化還元電位が嫌気性原生動物の優占種に及ぼす影響2015

    • Author(s)
      石塚充朗,平片悠河,荒木信夫,押木守
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢大学角間キャンパス(石川県・金沢市)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] UASB槽内での嫌気的硫黄酸化反応に与えるORPの影響調査および微生物群集構造解析2015

    • Author(s)
      TRAN THI THANH THUY,大槻洸太,黒田恭平,中村明靖,幡本将史,山口隆司,荒木信夫
    • Organizer
      第41回関東支部技術研究発表会発表概要集
    • Place of Presentation
      アオーレ長岡(新潟県・長岡市)
    • Year and Date
      2015-03-13 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effects of anaerobic protozoa on treatment efficiency and microbial community structure2014

    • Author(s)
      Y.Hirakata, M.Oshiki, N.Araki, K.Kuroda, M.Hatamoto, T.Yamaguchi
    • Organizer
      The 4th International Symposium on Tchnology for Sustainability
    • Place of Presentation
      National Taipei University of Technology(台湾・台北)
    • Year and Date
      2014-11-19 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 次世代シークエンサーMiseqによる嫌気性原生動物の共生最近叢の解析2014

    • Author(s)
      石塚充朗,平片悠河,荒木信夫,幡本将史,山口隆司
    • Organizer
      第32回土木学会関東支部新潟会研究調査発表会
    • Place of Presentation
      ハイブ長岡(新潟県・長岡市)
    • Year and Date
      2014-11-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 都市下水処理UASB槽内の嫌気性原生動物が処理性能に及ぼす影響2014

    • Author(s)
      辻澤伊吹,平片悠河,荒木信夫,土田真実子,山口隆司
    • Organizer
      第32回土木学会関東支部新潟会研究調査発表会
    • Place of Presentation
      ハイブ長岡(新潟県・長岡市)
    • Year and Date
      2014-11-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 嫌気性原生動物細胞内に共生する難培養性バクテリア、・アーキアの分子生物学的解析2014

    • Author(s)
      平片悠河,押木守,荒木信夫,黒田恭平,幡本将史,山口隆司
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      静岡大学(静岡県・浜松市)
    • Year and Date
      2014-10-22 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 嫌気性リアクターを用いた硫黄の嫌気的酸化還元に与えるORPの影響2014

    • Author(s)
      TRAN THI THANH THUY, 大槻洸太, 黒田恭平, 中村明靖, 幡本将史, 山口隆司, 山崎慎一, 荒木信夫
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      東北大学川内キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] UASBリアクター内における嫌気的硫黄酸化反応と供給基質の関係2014

    • Author(s)
      大槻洸太, TRAN THI THANH THUY, 黒田恭平, 中村明靖, 幡本将史, 山口隆司, 山崎慎一, 荒木信夫
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      東北大学川内キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 走電性を用いた嫌気性原生動物の分離とその解析2014

    • Author(s)
      佐藤由佳,平片悠河,荒木信夫,黒田恭平,山口隆司,幡本将史
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      東北大学川内キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 嫌気性原生動物の共存が都市下水処理UASBの処理性能に与える影響2014

    • Author(s)
      土田真実子,平片悠河,荒木信夫,山口隆司, 幡本将史
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      東北大学川内キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] UASB+DHS を用いた都市下水処理システムにおける嫌気無酸素回分式リアクターを用いた窒素除去およびリン除去・回収2013

    • Author(s)
      坂本健一,幡本将史,山口隆司,高橋優信,久保田健吾,原田秀樹,荒木信夫,山崎慎一,大久保努,上村繁樹
    • Organizer
      土木学会第68回年次学術講演会
    • Place of Presentation
      日本大学生産工学部
    • Related Report
      2013 Annual Research Report
  • [Presentation] DHSリアクターのスポンジ担体保持汚泥に生息する真核生物の解析2013

    • Author(s)
      宮岡佑馬,幡本将史,山口隆司,荒木信夫,多川正,小野寺崇,珠坪一晃
    • Organizer
      土木学会第68回年次学術講演会
    • Place of Presentation
      日本大学生産工学部
    • Related Report
      2013 Annual Research Report
  • [Presentation] 嫌気性原生動物の共生の有無がUASB槽内の細菌叢に与える影響2013

    • Author(s)
      土田真実子,平片悠河,荒木信夫,関由梨絵,山口隆司
    • Organizer
      第31回土木学会関東支部新潟会研究調査発表会
    • Place of Presentation
      ハイブ長岡(長岡市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] UASB槽内に生息する嫌気性原生動物の群集構造解析2013

    • Author(s)
      佐藤由佳,平片悠河,荒木信夫,山口隆司
    • Organizer
      第31回土木学会関東支部新潟会研究調査発表会
    • Place of Presentation
      ハイブ長岡(長岡市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 原生動物の走電性を用いた単離手法の開発2013

    • Author(s)
      宮島浩輔,平片悠河,荒木信夫,島津美砂子,山口隆司
    • Organizer
      第31回土木学会関東支部新潟会研究調査発表会
    • Place of Presentation
      ハイブ長岡(長岡市)
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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