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Bio-MEMS with reversible antibody on sensor chip

Research Project

Project/Area Number 25289290
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTohoku University

Principal Investigator

Umetsu Mitsuo  東北大学, 工学(系)研究科(研究院), 教授 (70333846)

Co-Investigator(Kenkyū-buntansha) MATSUI JUN  山形大学, 理学部, 准教授 (50361184)
NAGAMINE KUNIAKI  東北大学, 大学院工学研究科, 助教 (00551540)
Co-Investigator(Renkei-kenkyūsha) NISHIZAWA MASAHIKO  東北大学, 大学院工学研究科, 教授 (20273592)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsペプチド / タンパク質 / ナノバイオ / 抗体 / MEMS
Outline of Final Research Achievements

Microelectromechanical system for biology has the potential for reconstituting chain chemical reaction and protein-protein interaction in cell. However, for the construction of cell in MEMS, The ways to immobilize a protein on a specific position on MEMS and to irrepressibly immobilize proteins on MEMS should be studies. In this study, we evaluated material-binding peptide to the peptide with reversible binding to material surfaces. The peptide could be reversibly bound by the change of ionic strength in solution.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (31 results)

All 2015 2014 2013

All Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) (of which Int'l Joint Research: 4 results,  Invited: 9 results) Book (1 results)

  • [Journal Article] Organic crystal-binding peptides: Morphology control and one-pot formation of protein-displaying organic crystals2015

    • Author(s)
      T. Niide, K. Ozawa, H. Nakazawa, D. Oliveira, H. Kasai, M. Onodera, R. Asano, I. Kumagai, M. Umetsu
    • Journal Title

      Nanoscale

      Volume: 7 Issue: 47 Pages: 20155-20163

    • DOI

      10.1039/c5nr06471f

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Behavior of Kinesin Driven Quantum Dots Trapped in a Microtubule Loop2015

    • Author(s)
      Aurelien Sikora, Filippo Federici Canova, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Wonmuk Hwang, Winfried Teizer
    • Journal Title

      ACS Nano

      Volume: 9 Issue: 11 Pages: 11003-11013

    • DOI

      10.1021/acsnano.5b04348

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microtubule guiding in a multi-walled carbon nanotube circuit2015

    • Author(s)
      Aurelien Sikora, Javier Ramon, Mustafa Sen, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Hitoshi Shiku, Tomokazu Matsue, Winfried Teizer
    • Journal Title

      Biomedical Microdevices

      Volume: 17 Issue: 4

    • DOI

      10.1007/s10544-015-9978-1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ナノとタンパク質のいい関係:利点を相補完するハイブリッドタンパク質設計2015

    • Author(s)
      梅津 光央
    • Journal Title

      化学と生物

      Volume: 54 Pages: 240-242

    • NAID

      130005464570

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Molecular Motor-Powered Shuttles along Multi-walled Carbon Nanotube Tracks2014

    • Author(s)
      Aurelien Sikora, Javier Ramon-Azcon, Kyongwan Kim, Kelley Reaves, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Hitoshi Shiku, Tomokazu Matsue, Wonmuk Hwang, Winfried Teizer
    • Journal Title

      Nano Letters

      Volume: 14 Issue: 2 Pages: 876-881

    • DOI

      10.1021/nl4042388

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional localization of kinesin/microtubule-based motility systemalong metallic glass microwires2014

    • Author(s)
      Kyongwan Kim, Aurélien Sikora, Koji S. Nakayama, Hikaru Nakazawa, Mitsuo Umetsu, Wonmuk Hwang, and Winfried Teizer
    • Journal Title

      Applied Physics Letters

      Volume: 105 Issue: 14 Pages: 143701-143701

    • DOI

      10.1063/1.4896964

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of 300x Enhanced Fluorescence on a Plasmonic Chip Modified with a Bispecific Antibody to a Sensitive Immunosensor2013

    • Author(s)
      Keiko Tawa, Mitsuo Umetsu, Hikaru Nakazawa, Takamitsu Hattori, Izumi Kumagai
    • Journal Title

      ACS Applied Materials & Interfaces

      Volume: 5 Issue: 17 Pages: 8628-8632

    • DOI

      10.1021/am402173y

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新規巻き戻し法による標的タンパク質の調製 ―完全変性を経由しない巻き戻し法―2013

    • Author(s)
      梅津 光央、熊谷 泉
    • Journal Title

      BIO INDUSTRY

      Volume: 30 Pages: 113-122

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Hybrid Nanocellulosome Design from Cellulase Modules on Nanoparticles : Synergistic Effect of Catalytically Divergent Cellulase Modules on Cellulose Degradation Activity2013

    • Author(s)
      Hikaru Nakazawa, Do-Myoung Kim, Takeshi Matsuyama, Nobuhiro Ishida, Akinori Ikeuchi, Yuri Ishigaki, Izumi Kumagai, Mitsuo Umetsu
    • Journal Title

      ACS Catalysis

      Volume: 3 Issue: 6 Pages: 1342-1348

    • DOI

      10.1021/cs400012v

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biomass-binding peptides designed by molecular evolution for efficient degradation of cellulose in biomass by cellulase2013

    • Author(s)
      Hikaru Nakazawa, Akinori Ikeuchi, Do-Myoung Kim, Yuri Ishigaki, Hidetaka Asano, Katsunori Kouda, Izumi Kumagai, Mitsuo Umetsu
    • Journal Title

      Green Chemistry

      Volume: 15 Issue: 2 Pages: 365-369

    • DOI

      10.1039/c2gc36914a

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In-one-pot-at-a-time ligation for high-throughput construction of a protein expression vector library2013

    • Author(s)
      Hikaru Nakazawa, Rui Todokoro, Yuri Ishigaki, Izumi Kumagai, Mitsuo Umetsu
    • Journal Title

      Chemistry letters

      Volume: 42 Pages: 424-426

    • NAID

      10031162195

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Recombinant small antibody design for plasmonic biosensor using camel antibody with affinity for target inorganic material2015

    • Author(s)
      Takuma Sujino, Hikaru Nakazawa, Keiko Tawa, Ryutaro Asano, Izumi Kumagai, Mitsuo Umetsu
    • Organizer
      Pacifichem2015
    • Place of Presentation
      Honolulu (USA)
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Module library approach for bottom-up chimera/hybrid protein design2015

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      The 6th iBioK Asian Workshop
    • Place of Presentation
      Kobe University (Kobe)
    • Year and Date
      2015-12-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plasmonic and nanoporous membrane formation by material-binding bispecific antibodies2015

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      2st France-Japan Symposium on Green-Materials and Advanced Characterization
    • Place of Presentation
      University of Tsukuba (Tsukuba)
    • Year and Date
      2015-08-31
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Biotechnology meets inorganic nanomaterials2015

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      Tohoku University's chemistry Summer School 2015
    • Place of Presentation
      Tohoku Univ. (Sendai)
    • Year and Date
      2015-08-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] タンパク質工学とナノ材料工学の相補完によるボトムアップ素子設計2015

    • Author(s)
      梅津 光央
    • Organizer
      ナノ学会第13回大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2015-05-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Nanoporous gold membrane from ZnO-Au nanoparticle assembly induced by material-binding bispecific antibodies2015

    • Author(s)
      Mitsuo Umetsu, Teppei Niide, Noriyoshi Manabe, Hikaru Nakazawa, Izumi Kumagai
    • Organizer
      Hybrid Materials 2015
    • Place of Presentation
      Sites (Spain)
    • Year and Date
      2015-03-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] スマートバイオデザイン:ガラパゴスなバイオ分子を使いこなす2015

    • Author(s)
      梅津 光央
    • Organizer
      山形大学理学部セミナー
    • Place of Presentation
      山形大学 (山形市)
    • Year and Date
      2015-02-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ペプチドから抗体そしてインターフェイス分子への階層設計:ナノ世界の糊として2014

    • Author(s)
      梅津 光央
    • Organizer
      第37回分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Smart interface antibody design for biosensor2014

    • Author(s)
      筋野 拓馬, 笹川 知里, 中澤 光, 田和 圭子, 浅野 竜太郎, 熊谷 泉, 梅津 光央
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター (札幌)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 表面プラズモン励起増強チップに最適な検出抗体のスマート設計2014

    • Author(s)
      筋野 拓馬, 笹川 知里, 中澤 光, 田和 圭子, 浅野 竜太郎, 熊谷 泉, 梅津 光央
    • Organizer
      第8回バイオ関連シンポジウム
    • Place of Presentation
      岡山大学 (岡山市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Convenient antibody design for plasmonic biosensor using camel antibody with affinity for inorganic material.2014

    • Author(s)
      Takuma Sujino, Hikaru Nakazawa, Keiko Tawa, Ryutaro Asano, Izumi Kumagai, Mitsuo Umetsu
    • Organizer
      248th ACS National Meeting & Exposition Submission Confirmation
    • Place of Presentation
      San Francisco (USA)
    • Year and Date
      2014-08-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 無機基板を抗原としたラクダ抗体から発想するバイオセンサー指向二重特異性抗体の設計2014

    • Author(s)
      筋野 拓馬, 笹川 知里, 中澤 光, 田和 圭子, 浅野 竜太郎, 熊谷 泉, 梅津 光央
    • Organizer
      生体機能関連化学部会若手の会 第26回サマースクール
    • Place of Presentation
      ラフォーレ蔵王 (刈田郡蔵王町)
    • Year and Date
      2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 無機基板を抗原としたラクダ抗体から発想するバイオセンサー指向インターフェイス設計2014

    • Author(s)
      筋野 拓馬, 中澤 光, 田和 圭子, 浅野 竜太郎, 熊谷 泉, 梅津 光央
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      横浜産貿ホールマリネリア (横浜市)
    • Year and Date
      2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 一本鎖抗体の自発的二量体化から発想するラクダ可変領域シングルドメインを利用した多重特異性多価抗体2014

    • Author(s)
      藤井 滉人, 筋野 拓馬, 中澤 光, 真鍋 法義, 浅野 竜太郎, 熊谷 泉, 梅津 光央
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学 (名古屋市)
    • Year and Date
      2014-03-27
    • Related Report
      2013 Annual Research Report
  • [Presentation] 一本鎖抗体の自己会合特性を活かした多価・多重特異性抗体設計2014

    • Author(s)
      梅津 光央, 藤井 滉人, 筋野 拓馬, 真鍋 法義, 中澤 光, 田中 良和, 浅野 竜太郎, 熊谷 泉
    • Organizer
      化学工学会第79年会
    • Place of Presentation
      岐阜大学 (岐阜市)
    • Year and Date
      2014-03-20
    • Related Report
      2013 Annual Research Report
  • [Presentation] 抗体フォールドを用いたインターフェイスデザイン:ナノ世界の糊として2013

    • Author(s)
      梅津 光央
    • Organizer
      未来化学創造センター ナノバイオアセンブリワークショップ
    • Place of Presentation
      福岡市産学連携交流センター (福岡)
    • Year and Date
      2013-11-15
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Smart bio-design for interface molecules between nanomaterials2013

    • Author(s)
      Mitsuo Umetsu
    • Organizer
      日本化学会東北支部70周年記念国際会議
    • Place of Presentation
      東北大学 (仙台市)
    • Year and Date
      2013-09-28
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ナノを意識した蛋白質スマートデザイン2013

    • Author(s)
      梅津 光央
    • Organizer
      生体機能関連化学部会 第28回若手フォーラム
    • Place of Presentation
      名古屋大学 (名古屋市)
    • Year and Date
      2013-09-26
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 材料標的抗体を錨としたバイオセンサー指向インターフェイス設計2013

    • Author(s)
      梅津光央, 田和圭子, 中澤光, 筋野拓馬, 服部峰充
    • Organizer
      化学工学会 第45回秋季大会
    • Place of Presentation
      岡山大学 (岡山市)
    • Year and Date
      2013-09-16
    • Related Report
      2013 Annual Research Report
  • [Book] 進化分子工学2013

    • Author(s)
      梅津 光央、中澤 光、服部 峰充
    • Total Pages
      424
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2017-05-10  

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