Bio-MEMS with reversible antibody on sensor chip

• Project/Area Number: 25289290
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Tohoku University
• Principal Investigator: Umetsu Mitsuo 東北大学, 工学(系)研究科(研究院), 教授 (70333846)
• Co-Investigator(Kenkyū-buntansha): MATSUI JUN 山形大学, 理学部, 准教授 (50361184) ; NAGAMINE KUNIAKI 東北大学, 大学院工学研究科, 助教 (00551540)
• Co-Investigator(Renkei-kenkyūsha): NISHIZAWA MASAHIKO 東北大学, 大学院工学研究科, 教授 (20273592)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: ペプチド / タンパク質 / ナノバイオ / 抗体 / MEMS

Outline of Final Research Achievements

Microelectromechanical system for biology has the potential for reconstituting chain chemical reaction and protein-protein interaction in cell. However, for the construction of cell in MEMS, The ways to immobilize a protein on a specific position on MEMS and to irrepressibly immobilize proteins on MEMS should be studies. In this study, we evaluated material-binding peptide to the peptide with reversible binding to material surfaces. The peptide could be reversibly bound by the change of ionic strength in solution.

Research Products

• [Journal Article] Organic crystal-binding peptides: Morphology control and one-pot formation of protein-displaying organic crystals (2015)
  • Author(s): T. Niide, K. Ozawa, H. Nakazawa, D. Oliveira, H. Kasai, M. Onodera, R. Asano, I. Kumagai, M. Umetsu
  • Journal Title: Nanoscale Volume: 7 Issue: 47 Pages: 20155-20163
  • DOI: 10.1039/c5nr06471f
  • Peer Reviewed
• [Journal Article] Behavior of Kinesin Driven Quantum Dots Trapped in a Microtubule Loop (2015)
  • Author(s): Aurelien Sikora, Filippo Federici Canova, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Wonmuk Hwang, Winfried Teizer
  • Journal Title: ACS Nano Volume: 9 Issue: 11 Pages: 11003-11013
  • DOI: 10.1021/acsnano.5b04348
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Microtubule guiding in a multi-walled carbon nanotube circuit (2015)
  • Author(s): Aurelien Sikora, Javier Ramon, Mustafa Sen, Kyongwan Kim, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Hitoshi Shiku, Tomokazu Matsue, Winfried Teizer
  • Journal Title: Biomedical Microdevices Volume: 17 Issue: 4
  • DOI: 10.1007/s10544-015-9978-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] ナノとタンパク質のいい関係：利点を相補完するハイブリッドタンパク質設計 (2015)
  • Author(s): 梅津 光央
  • Journal Title: 化学と生物 Volume: 54 Pages: 240-242
  • NAID: 130005464570
• [Journal Article] Molecular Motor-Powered Shuttles along Multi-walled Carbon Nanotube Tracks (2014)
  • Author(s): Aurelien Sikora, Javier Ramon-Azcon, Kyongwan Kim, Kelley Reaves, Hikaru Nakazawa, Mitsuo Umetsu, Izumi Kumagai, Tadafumi Adschiri, Hitoshi Shiku, Tomokazu Matsue, Wonmuk Hwang, Winfried Teizer
  • Journal Title: Nano Letters Volume: 14 Issue: 2 Pages: 876-881
  • DOI: 10.1021/nl4042388
  • Peer Reviewed
• [Journal Article] Functional localization of kinesin/microtubule-based motility systemalong metallic glass microwires (2014)
  • Author(s): Kyongwan Kim, Aurélien Sikora, Koji S. Nakayama, Hikaru Nakazawa, Mitsuo Umetsu, Wonmuk Hwang, and Winfried Teizer
  • Journal Title: Applied Physics Letters Volume: 105 Issue: 14 Pages: 143701-143701
  • DOI: 10.1063/1.4896964
  • Peer Reviewed
• [Journal Article] Application of 300x Enhanced Fluorescence on a Plasmonic Chip Modified with a Bispecific Antibody to a Sensitive Immunosensor (2013)
  • Author(s): Keiko Tawa, Mitsuo Umetsu, Hikaru Nakazawa, Takamitsu Hattori, Izumi Kumagai
  • Journal Title: ACS Applied Materials & Interfaces Volume: 5 Issue: 17 Pages: 8628-8632
  • DOI: 10.1021/am402173y
  • Peer Reviewed
• [Journal Article] 新規巻き戻し法による標的タンパク質の調製 ―完全変性を経由しない巻き戻し法― (2013)
  • Author(s): 梅津 光央、熊谷 泉
  • Journal Title: BIO INDUSTRY Volume: 30 Pages: 113-122
• [Journal Article] Hybrid Nanocellulosome Design from Cellulase Modules on Nanoparticles : Synergistic Effect of Catalytically Divergent Cellulase Modules on Cellulose Degradation Activity (2013)
  • Author(s): Hikaru Nakazawa, Do-Myoung Kim, Takeshi Matsuyama, Nobuhiro Ishida, Akinori Ikeuchi, Yuri Ishigaki, Izumi Kumagai, Mitsuo Umetsu
  • Journal Title: ACS Catalysis Volume: 3 Issue: 6 Pages: 1342-1348
  • DOI: 10.1021/cs400012v
  • Peer Reviewed
• [Journal Article] Biomass-binding peptides designed by molecular evolution for efficient degradation of cellulose in biomass by cellulase (2013)
  • Author(s): Hikaru Nakazawa, Akinori Ikeuchi, Do-Myoung Kim, Yuri Ishigaki, Hidetaka Asano, Katsunori Kouda, Izumi Kumagai, Mitsuo Umetsu
  • Journal Title: Green Chemistry Volume: 15 Issue: 2 Pages: 365-369
  • DOI: 10.1039/c2gc36914a
  • Peer Reviewed
• [Journal Article] In-one-pot-at-a-time ligation for high-throughput construction of a protein expression vector library (2013)
  • Author(s): Hikaru Nakazawa, Rui Todokoro, Yuri Ishigaki, Izumi Kumagai, Mitsuo Umetsu
  • Journal Title: Chemistry letters Volume: 42 Pages: 424-426
  • NAID: 10031162195
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Recombinant small antibody design for plasmonic biosensor using camel antibody with affinity for target inorganic material (2015)
  • Author(s): Takuma Sujino, Hikaru Nakazawa, Keiko Tawa, Ryutaro Asano, Izumi Kumagai, Mitsuo Umetsu
  • Organizer: Pacifichem2015
  • Place of Presentation: Honolulu (USA)
  • Year and Date: 2015-12-18
  • Int'l Joint Research
• [Presentation] Module library approach for bottom-up chimera/hybrid protein design (2015)
  • Author(s): Mitsuo Umetsu
  • Organizer: The 6th iBioK Asian Workshop
  • Place of Presentation: Kobe University (Kobe)
  • Year and Date: 2015-12-07
  • Int'l Joint Research / Invited
• [Presentation] Plasmonic and nanoporous membrane formation by material-binding bispecific antibodies (2015)
  • Author(s): Mitsuo Umetsu
  • Organizer: 2st France-Japan Symposium on Green-Materials and Advanced Characterization
  • Place of Presentation: University of Tsukuba (Tsukuba)
  • Year and Date: 2015-08-31
  • Int'l Joint Research / Invited
• [Presentation] Biotechnology meets inorganic nanomaterials (2015)
  • Author(s): Mitsuo Umetsu
  • Organizer: Tohoku University's chemistry Summer School 2015
  • Place of Presentation: Tohoku Univ. (Sendai)
  • Year and Date: 2015-08-28
  • Int'l Joint Research / Invited
• [Presentation] タンパク質工学とナノ材料工学の相補完によるボトムアップ素子設計 (2015)
  • Author(s): 梅津 光央
  • Organizer: ナノ学会第13回大会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2015-05-11
  • Invited
• [Presentation] Nanoporous gold membrane from ZnO-Au nanoparticle assembly induced by material-binding bispecific antibodies (2015)
  • Author(s): Mitsuo Umetsu, Teppei Niide, Noriyoshi Manabe, Hikaru Nakazawa, Izumi Kumagai
  • Organizer: Hybrid Materials 2015
  • Place of Presentation: Sites (Spain)
  • Year and Date: 2015-03-12
• [Presentation] スマートバイオデザイン：ガラパゴスなバイオ分子を使いこなす (2015)
  • Author(s): 梅津 光央
  • Organizer: 山形大学理学部セミナー
  • Place of Presentation: 山形大学 (山形市)
  • Year and Date: 2015-02-10
  • Invited
• [Presentation] ペプチドから抗体そしてインターフェイス分子への階層設計：ナノ世界の糊として (2014)
  • Author(s): 梅津 光央
  • Organizer: 第37回分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2014-11-26
  • Invited
• [Presentation] Smart interface antibody design for biosensor (2014)
  • Author(s): 筋野 拓馬, 笹川 知里, 中澤 光, 田和 圭子, 浅野 竜太郎, 熊谷 泉, 梅津 光央
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター (札幌)
  • Year and Date: 2014-09-26
• [Presentation] 表面プラズモン励起増強チップに最適な検出抗体のスマート設計 (2014)
  • Author(s): 筋野 拓馬, 笹川 知里, 中澤 光, 田和 圭子, 浅野 竜太郎, 熊谷 泉, 梅津 光央
  • Organizer: 第8回バイオ関連シンポジウム
  • Place of Presentation: 岡山大学 (岡山市)
  • Year and Date: 2014-09-12
• [Presentation] Convenient antibody design for plasmonic biosensor using camel antibody with affinity for inorganic material. (2014)
  • Author(s): Takuma Sujino, Hikaru Nakazawa, Keiko Tawa, Ryutaro Asano, Izumi Kumagai, Mitsuo Umetsu
  • Organizer: 248th ACS National Meeting & Exposition Submission Confirmation
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2014-08-12
• [Presentation] 無機基板を抗原としたラクダ抗体から発想するバイオセンサー指向二重特異性抗体の設計 (2014)
  • Author(s): 筋野 拓馬, 笹川 知里, 中澤 光, 田和 圭子, 浅野 竜太郎, 熊谷 泉, 梅津 光央
  • Organizer: 生体機能関連化学部会若手の会 第26回サマースクール
  • Place of Presentation: ラフォーレ蔵王 (刈田郡蔵王町)
  • Year and Date: 2014-07-25
• [Presentation] 無機基板を抗原としたラクダ抗体から発想するバイオセンサー指向インターフェイス設計 (2014)
  • Author(s): 筋野 拓馬, 中澤 光, 田和 圭子, 浅野 竜太郎, 熊谷 泉, 梅津 光央
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: 横浜産貿ホールマリネリア (横浜市)
  • Year and Date: 2014-06-27
• [Presentation] 一本鎖抗体の自発的二量体化から発想するラクダ可変領域シングルドメインを利用した多重特異性多価抗体 (2014)
  • Author(s): 藤井 滉人, 筋野 拓馬, 中澤 光, 真鍋 法義, 浅野 竜太郎, 熊谷 泉, 梅津 光央
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学 (名古屋市)
  • Year and Date: 2014-03-27
• [Presentation] 一本鎖抗体の自己会合特性を活かした多価・多重特異性抗体設計 (2014)
  • Author(s): 梅津 光央, 藤井 滉人, 筋野 拓馬, 真鍋 法義, 中澤 光, 田中 良和, 浅野 竜太郎, 熊谷 泉
  • Organizer: 化学工学会第79年会
  • Place of Presentation: 岐阜大学 (岐阜市)
  • Year and Date: 2014-03-20
• [Presentation] 抗体フォールドを用いたインターフェイスデザイン：ナノ世界の糊として (2013)
  • Author(s): 梅津 光央
  • Organizer: 未来化学創造センター ナノバイオアセンブリワークショップ
  • Place of Presentation: 福岡市産学連携交流センター (福岡)
  • Year and Date: 2013-11-15
  • Invited
• [Presentation] Smart bio-design for interface molecules between nanomaterials (2013)
  • Author(s): Mitsuo Umetsu
  • Organizer: 日本化学会東北支部７０周年記念国際会議
  • Place of Presentation: 東北大学 (仙台市)
  • Year and Date: 2013-09-28
  • Invited
• [Presentation] ナノを意識した蛋白質スマートデザイン (2013)
  • Author(s): 梅津 光央
  • Organizer: 生体機能関連化学部会 第28回若手フォーラム
  • Place of Presentation: 名古屋大学 (名古屋市)
  • Year and Date: 2013-09-26
  • Invited
• [Presentation] 材料標的抗体を錨としたバイオセンサー指向インターフェイス設計 (2013)
  • Author(s): 梅津光央, 田和圭子, 中澤光, 筋野拓馬, 服部峰充
  • Organizer: 化学工学会 第45回秋季大会
  • Place of Presentation: 岡山大学 (岡山市)
  • Year and Date: 2013-09-16
• [Book] 進化分子工学 (2013)
  • Author(s): 梅津 光央、中澤 光、服部 峰充
  • Total Pages: 424
  • Publisher: エヌ・ティー・エス