Engineering three dimensional tissues using electrochemistry
| Project/Area Number |
25289291
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Yokohama National University |
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Principal Investigator |
Fukuda Junji 横浜国立大学, 工学(系)研究科(研究院), 准教授 (80431675)
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| Co-Investigator(Kenkyū-buntansha) |
Wang Picao 筑波大学, 生命環境科学研究科, 教授 (80261775)
Watanabe Masatoshi 横浜国立大学, 工学研究院, 教授 (90273383)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | オリゴペプチド / 分子動力学計算 / ニードル / Hep G2 / 管腔形成 / 電気化学 / 自己組織化 / 肝細胞 / 血管内皮細胞 / 細胞シート / 電気化学細胞脱離 |
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| Outline of Final Research Achievements |
An oligopeptide (CAAAEKEKEKEKGRGDSP) was designed using molecular dynamics calculations to electrochemically detach cells from a culture surface. Vascular endothelial cells attached on needles via the oligopeptide layer were electrochemically detached from the needles and transferred to surrounding hydrogel, resulting in endothelialized microchannels. Further, hepatoblastoma Hep G2 cells were previously encapsulated in the hydrogel. Hep G2 cells self-organized with vasculatures in following perfusion culture and formed vascularized hepatic tissues in vitro.
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Report
(4 results)
Research Products
(89 results)
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[Journal Article] Generation of proliferating lung progenitor-like cells form fibroblasts with defined transcription factors.2016
Author(s)
N.Ninomiya, T.K.Noguchi, M.Sekine, M. Mochizuki, K.Fukui, P.C.Wang, M. Asashima, K. Horimoto, A. Kurisaki,
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Journal Title
Stem Cell,
Volume: in press
Issue: 1
Pages: 95-105
DOI
Related Report
Peer Reviewed
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[Book] 実験医学2015
Author(s)
大崎達哉、福田淳二、小池博之、武部貴則
Total Pages
6
Publisher
羊土社
Related Report
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