| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
An oligopeptide (CAAAEKEKEKEKGRGDSP) was designed using molecular dynamics calculations to electrochemically detach cells from a culture surface. Vascular endothelial cells attached on needles via the oligopeptide layer were electrochemically detached from the needles and transferred to surrounding hydrogel, resulting in endothelialized microchannels. Further, hepatoblastoma Hep G2 cells were previously encapsulated in the hydrogel. Hep G2 cells self-organized with vasculatures in following perfusion culture and formed vascularized hepatic tissues in vitro.
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