Regulation of gene expression by the cytoplasmic germ granules in primordial germ cells

• Project/Area Number: 25290076
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: System genome science
• Research Institution: Kobe University
• Principal Investigator: INOUE KUNIO 神戸大学, 理学(系)研究科(研究院), 教授 (40252415)
• Research Collaborator: HOSHIJIMA Kazuyuki 米国ユタ大学, 助教
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 生殖細胞 / RNA / ゲノム情報

Outline of Final Research Achievements

The mechanism underlying how germ cell-specific gene expression is regulated by the cytoplasmic determinant "germ granules" that is maternally supplied remains to be elucidated. In this study, we performed RNA-seq analyses of the wild type and germ cell-deficient embryos and identified many candidates that may act in germ cell-specific transcriptional regulation. In addition, we showed that a germ cell-specific RNA-binding protein DND has the ability to repress translation of target mRNAs in zebrafish.

Research Products

• [Int'l Joint Research] ユタ大学(米国)
• [Journal Article] Interaction and colocalization of HERMES/RBPMS with NonO, PSF, and G3BP1 in neuronal cytoplasmic RNP granules in mouse retinal line cells. (2015)
  • Author(s): Furukawa, M.T., Sakamoto, H., and Inoue, K.
  • Journal Title: Genes to Cells Volume: 20 Issue: 4 Pages: 257-266
  • DOI: 10.1111/gtc.12224
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The origin and migration of primordial germ cells in sturgeons (2014)
  • Author(s): Saito T., Psenicka M., Goto R., Adachi S., Inoue K., Arai K., Yamaha E.
  • Journal Title: PLoS ONE Volume: 9-2 Issue: 2 Pages: e86861-e86861
  • DOI: 10.1371/journal.pone.0086861
  • NAID: 120005372288
  • Peer Reviewed
• [Presentation] ゼブラフィッシュ卵母細胞のmRNA輸送機構の解析 (2015)
  • Author(s): 髙松 陽紀、坂本 博、井上 邦夫
  • Organizer: 第38回日本分子生物学会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2015-12-01
• [Presentation] Identification of mRNAs regulated by the germ granule protein Dead-end in zebrafish by RNA-seq. (2015)
  • Author(s): 小林 真奈美、井上 邦夫
  • Organizer: 第17回日本RNA学会
  • Place of Presentation: ホテルライフォート札幌
  • Year and Date: 2015-07-15
• [Presentation] Identification of mRNAs regulated by the germ granule protein Dead-endin zebrafish by RNA-seq. (2015)
  • Author(s): Kobayashi M. , Inoue K.
  • Organizer: 9th European Zebrafish Meeting
  • Place of Presentation: Oslo Congress Centre, Norway
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] Identification of mRNAs regulated by the germ granule protein Dead-end in zebrafish by RNA-seq. (2015)
  • Author(s): 小林 真奈美、井上 邦夫
  • Organizer: 第48回日本発生生物学会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2015-06-02
• [Presentation] ゼブラフィッシュ生殖顆粒校正因子Dead-endにより発現制御を受けるmRNA群の同定と機能解析 (2014)
  • Author(s): 小林真奈美、井上邦夫
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 線虫C.elegansにおけるクロモドメイン蛋白質MRG-1の始原生殖細胞への限局メカニズムの解析 (2014)
  • Author(s): 巳波孝至、井上邦夫、坂本博、高崎輝恒
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27