Structural basis of prevention by RNA aptamer of transformation of prion protein and its interaction with Ab
| Project/Area Number |
25291013
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
Katahira Masato 京都大学, エネルギー理工学研究所, 教授 (70211844)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
NAGATA Takashi 京都大学, エネルギー理工学研究所, 准教授 (10415250)
MASHIMA Tsukasa 京都大学, エネルギー理工学研究所, 助教 (20707426)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
|
|---|
| Keywords | Ab蛋白質 / プリオン蛋白質 / RNAアプタマー / 機能性核酸 / NMR / アミロイドβ / プリオン / 機能性ＲＮＡ / アルツハイマー病 / 線維化 / 分子間相互作用 / アミロイドβタンパク質 / 抗プリオン活性 / 分子認識 / プリオンタンパク質 / ＲＮＡアプタマー / ＮＭＲ / 相互作用 / プリオン病 |
|---|
| Outline of Final Research Achievements |
The Ab protein, that is supposed to be responsible for the Alzheimer's disease, forms the fibril. Recently it was proposed that the prion protein serves as a receptor of the Ab protein. This time, we studied the effect of the prion protein and the RNA aptamer against the prion protein on fibril formation of the Ab protein. First, it was confirmed that the Ab protein forms the fibril. Secondary, it was revealed that the prion protein interferes with the fibril formation of the Ab protein. Thirdly, it was demonstrated that the RNA aptamer against the prion protein cancels the interfering effect of the prion protein and restores the fibril formation of the Ab protein. Additionally, the techniques developed to study the structure and interactions of nucleic acids such as the RNA aptamer were applied to other nucleic acids. Then, the correlation between the unique structure of the nucleic acids and their function were elucidated.
|
Report
(4 results)
Research Products
(14 results)
-
-
-
-
-
[Journal Article] Anti-prion activity of an RNA aptamer and its structural basis2013
Author(s)
Mashima, T., Nishikawa, F., Kamatari Y. O., Fujiwara, H., Saimura, M., Nagata, T., Kodaki, T., Nishikawa, S., Kuwata, K., Katahira, M.
-
Journal Title
Nucleic Acids Research
Volume: 41
Issue: 2
Pages: 1355-1362
DOI
Related Report
Peer Reviewed
-
-
-
-
[Presentation] Origin of the anti-prion activity of quadruplex2013
Author(s)
Tsukasa Mashima, Fumiko Nishikawa, Yuji O. Kamatari, Masayuki Saimura, Takashi Nagata, Satoshi Nishikawa, Kazuo Kuwata, Masato Katahira
Organizer
The 40th International Symposium on Nucleic Acids Chemistry 2013
Place of Presentation
神奈川大学(横浜市）
Related Report
-
[Presentation] The deamination of APOBEC3G is controlled by the sliding-direction-dependent catalytic activity, and anti-prion activity of an RNA aptamer and its structual basis
Author(s)
Ayako Furukawa, Kenji Sugase, Ryo Morishita, Takashi Nagata, Akifumi Takaori-Kondo, Akihide Ryo, Tsukasa Mashima, Fomiko Nishikawa, Satoshi Nishikawa, Yuji Kamatari, Kazuo Kuwata, Masato Katahira
Organizer
The 10th Korea-Japan Bilateral Symposium on Biological NMR
Place of Presentation
ソウル大学(ソウル、韓国）
Related Report
Invited
-
-
-
-
