A study on semaphorin-regulated diverse cellular events

• Project/Area Number: 25291044
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: Takagi Shin 名古屋大学, 理学(系)研究科(研究院), 准教授 (90171420)
• Co-Investigator(Renkei-kenkyūsha): IHARA Kunio 名古屋大学, 遺伝子実験施設, 准教授 (90223297)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: セマフォリン / 表皮 / エンドサイトシス / シナプトタグミン / 線虫 / 器官形成 / 細胞形態制御 / 膜小胞 / 線虫C. elegans

Outline of Final Research Achievements

In this study we have studied regulation of vesicle transport by the semaphorin signal in the nematode C. elegans. Through genetic as well as cell-biological analyses, we have demonstrated that semaphorin negatively regulates endocytosis in ray epidermal cells. We also revealed that the synaptotagmin1/Stonin2 system, a well-known regulator of synaptic vesicle recycling in neurons, plays a critical role in vesicle transport in ray epidermal cells. Our study shows importance of participation of the semaphorin-regulated vesicle transport in regulation of the cell shape.

Research Products

• [Journal Article] Astrocytic IP3Rs: Contribution to Ca2+ signalling and hippocampal LTP. (2017)
  • Author(s): Sherwood MW, Arizono M, Hisatsune C, Bannai H, Ebisui E, Sherwood JL, Panatier A, Oliet SH, Mikoshiba K.
  • Journal Title: Glia Volume: 65 Issue: 1 Pages: 502-513
  • DOI: 10.1016/j.bbrc.2016.09.034
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterizing Semaphorin Signaling in vivo using C. elegans (2017)
  • Author(s): Nukazuka A, Takagi S
  • Journal Title: Methods in Molecular Biology Volume: 1493 Pages: 485-498
  • DOI: 10.1007/978-1-4939-6448-2_34
  • ISBN: 9781493964468, 9781493964482
  • Peer Reviewed / Open Access
• [Journal Article] Optical silencing of C. elegans cells with light-driven proton pumps (2014)
  • Author(s): Ayako Okazaki, Megumi Takahashi, Naoya Toyoda, Shin Takagi
  • Journal Title: Methods Volume: 68 Issue: 3 Pages: 425-430
  • DOI: 10.1016/j.ymeth.2014.02.030
  • Peer Reviewed
• [Journal Article] Pulsed Irradiation Improves Target Selectivity of Infrared Laser-Evoked Gene Operator for Single-Cell Gene Induction in the Nematode C. elegans (2014)
  • Author(s): Suzuki M, Toyoda N, Takagi S
  • Journal Title: PLoS ONE Volume: 9(1) Issue: 1 Pages: e85783-e85783
  • DOI: 10.1371/journal.pone.0085783
  • Peer Reviewed / Open Access
• [Journal Article] IR Laser-induced gene expression in targeted single cells of C. elegans. Development (2013)
  • Author(s): Suzuki M,Toyoda N, Shimojyou M, Takagi S
  • Journal Title: Growth and Differentiation Volume: 55(4) Issue: 4 Pages: 454-61
  • DOI: 10.1111/dgd.12061
  • Peer Reviewed
• [Journal Article] Japanese studies on neural circuits and behavior of Caenorhabditis elegans. (2013)
  • Author(s): Sasakura, H., Tsukada, Y., Takagi, S. & Mori, I.
  • Journal Title: Front. Neural Circuits Volume: 7 Pages: 187-187
  • DOI: 10.3389/fncir.2013.00187
  • Peer Reviewed
• [Presentation] Analysis of morphological changes of epidermal cells producing sensory rays in the C. elegans male tail (2014)
  • Author(s): Shin Takagi, Hiroki Tanaka, Ayana Kobayashi
  • Organizer: C. elegans Development, Cell Biology and Gene Expression Meeting.
  • Place of Presentation: 奈良市 (新公会堂）
  • Year and Date: 2014-07-17
• [Presentation] Analysis of morphological changes of epidermal cells producing C. elegans sensory rays (2014)
  • Author(s): Shin Takagi, Hiroki Tanaka, Ayana Kobayashi
  • Organizer: 日本発生生物学会
  • Place of Presentation: 名古屋市 （ウィンク名古屋）
  • Year and Date: 2014-05-28
• [Presentation] Efficient EGL-17/FGF secretion by epidermal cells in C. elegans requires components of the synaptic vesicle recycling machinery
  • Author(s): 田中 洋樹，下条 真生、高木 新
  • Organizer: 日本発生生物学会大４６回大会
  • Place of Presentation: 松江 Kunibiki-messe
• [Remarks] Development and Neurobiology of C. elegans
  • URL: http://www.bio.nagoya-u.ac.jp/~zootwo/170313%20HP.htm