| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Outline of Final Research Achievements |
In this research project, we aimed to clarify the structure and function of the dynein calcium sensor calaxin. First, X-ray crystallography revealed that EF2/EF4 are structural and EF3 is a regulatory EF-hand. Overall calaxin structure was suggested to change by a Ca2+ from closed to open state, of which state change was largely accelerated by binding of dynein. By using sea urchin embryos, we concluded that calaxin determines basal body orientation by regulating ciliary bending. Calaxin-deficient mice possesse apparently normal axonemal structures. However, they represent typical ciliopathy phenotypes, in particular hydrocephalus and situs inversus.
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