The dynamics of X chromosome reactivation in female PGCs

• Project/Area Number: 25291076
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Kyoto University
• Principal Investigator: okamoto ikuhiro 京都大学, 医学(系)研究科(研究院), 講師 (40648424)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: マウス / X染色体 / 再活性化 / 始原生殖細胞 / 始源生殖細胞 / 不活性X染色体の再活性化 / 不活性X染色体 / リプログラミング / ゲノムリプログラミング

Outline of Final Research Achievements

XX females silence one of their X chromosomes. This involves a process whereby a noncoding RNA known as Xist coats one of the X chromosome. An exception to this occurs in pluripotent lineages, the inner cell mass (ICM) and primordial germ cells (PGCs), where the inactive X undergo reactivation. Analyzing PGC-like cells (PGCLCs) induced from mouse embryonic stem cells (ESCs), we found that hallmarks of the inactive X change, providing a direct readout of X reactivation progression.

Research Products

• [Journal Article] A developmental coordinate of pluripotency among mice, monkeys and humans. (2016)
  • Author(s): Nakamura T, Okamoto I, Sasaki K, Yabuta Y, Iwatani C, Tsuchiya H, Seita Y, Nakamura S, Yamamoto T, Saitou M.
  • Journal Title: Nature Volume: 537 Issue: 7618 Pages: 5762-5762
  • DOI: 10.1038/nature19096
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The Germ Cell Fate of Cynomolgus Monkeys Is Specified in the Nascent Amnion. (2016)
  • Author(s): Sasaki K, Nakamura T, Okamoto I, Yabuta Y, Iwatani C, Tsuchiya H, Seita Y, Nakamura S, Shiraki N, Takakuwa T, Yamamoto T, Saitou M.
  • Journal Title: Dev Cell. Volume: 39 Issue: 2 Pages: 169185-169185
  • DOI: 10.1016/j.devcel.2016.09.007
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells (2016)
  • Author(s): Ishikura Y, Yabuta Y, Ohta H, Hayashi K, Nakamura T, Okamoto I, Yamamoto T, Kurimoto K, Shirane K, Sasaki H, Saitou M
  • Journal Title: Cell Rep Volume: 17 Issue: 10 Pages: 2789-2804
  • DOI: 10.1016/j.celrep.2016.11.026
  • Peer Reviewed / Open Access
• [Journal Article] Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells (2015)
  • Author(s): Sasaki, K., Yokobayash, S., Nakamura, T., Okamoto, I., Yabuta, Y., Kurimoto, K., Ohta, H., Moritoki, Y., Iwatani, C., Tsuchiya, H., Nakamura, S., Sekiguchi, K., Sakuma, T., Yamamoto, T., Mori, T., Woltjen, K., Nakagawa, M., Yamamoto, T., Takahashi, K., Yamanaka, S., Saitou, M.
  • Journal Title: Cell Stem Cell Volume: 17 Issue: 2 Pages: 178-94
  • DOI: 10.1016/j.stem.2015.06.014
  • Peer Reviewed
• [Journal Article] SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression (2015)
  • Author(s): Nakamura, T., Yabuta, Y., Okamoto, I., Aramaki, S., Yokobayashi, S., Kurimoto, K., Sekiguchi, K., Nakagawa, M., Yamamoto, T., Saitou, M.
  • Journal Title: Nucleic Acids Res Volume: - Issue: 9 Pages: e60-e60
  • DOI: 10.1093/nar/gkv134
  • NAID: 120005825922
  • Peer Reviewed / Open Access
• [Journal Article] The Gpr1/Zdbf2 locus provides new paradigms for transient and dynamic genomic imprinting in mammals. (2014)
  • Author(s): Duffié R, Ajjan S, Greenberg MV, Zamudio N, Escamilla del Arenal M, Iranzo J, Okamoto I, Barbaux S, Fauque P, Bourc'his D
  • Journal Title: Genes Dev Volume: 1;28(5) Issue: 5 Pages: 463-78
  • DOI: 10.1101/gad.232058.113
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Two Active X Chromosomes in Female ESCs Block Exit from the Pluripotent State by Modulating the ESC Signaling Network (2014)
  • Author(s): Edda G. Schulzsend, Johannes Meisig, Tomonori Nakamura, Ikuhiro Okamoto, Anja Sieber, Christel Picard, Maud Borensztein, Mitinori Saitou, Nils Bluthgen, Edith Heard
  • Journal Title: Cell Stem Cell Volume: 14 Issue: 2 Pages: 203-216
  • DOI: 10.1016/j.stem.2013.11.022
  • Peer Reviewed
• [Journal Article] RNAi-dependent and independent control of LINE1 accumulation and mobility in mouse embryonic stem cells. (2013)
  • Author(s): Ciaudo C, Jay F, Okamoto I, Chen CJ, Sarazin A, Servant N, Barillot E, Heard E, Voinnet O.
  • Journal Title: PLoS Genet. Volume: 9 Issue: 11 Pages: 1-12
  • DOI: 10.1371/journal.pgen.1003791
  • Peer Reviewed
• [Presentation] Developmental dynamics of X-chromosome inactivation in the Cynomolgus monkey embryos (2016)
  • Author(s): 岡本郁弘
  • Organizer: X 染色体研究会
  • Place of Presentation: 鳥取大学 医学部（米子）
  • Year and Date: 2016-03-24
• [Presentation] Developmental dynamics of X-chromosome inactivation in eutherian (2015)
  • Author(s): 岡本郁弘
  • Organizer: X 染色体研究会
  • Place of Presentation: 近畿大学、農学部（奈良）
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Developmental dynamics of X-chromosome inactivation in eutherian (2014)
  • Author(s): 岡本郁弘
  • Organizer: 新学術領域『性差』若手研究集会
  • Place of Presentation: 静岡（熱海）
  • Year and Date: 2014-12-09 – 2014-12-10
  • Invited
• [Presentation] カニクイザル胚におけるX染色体不活性化のダイナミクス
  • Author(s): 岡本郁弘、佐々木恒太郎、中村友紀、熊木勇一、岩谷千鶴、土屋英明、斎藤通規
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場
  • Invited