Generation of plants resistant to DNA viruses using artificial DNA-binding proteins

• Project/Area Number: 25292036
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Okayama University
• Principal Investigator: SERA TAKASHI 岡山大学, 自然科学研究科, 教授 (10362443)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: ウイルス耐病性 / 植物DNAウイルス

Outline of Final Research Achievements

The goal of this study is to develop plants resistant to DNA viruses by inactivating them with artificial DNA-binding proteins. For this purpose, we designed an artificial DNA-binding protein to inactivate DNA virus of our interest and introduced the gene encoding the protein into plants, resulting in generation of the transgenic plants. Furthermore, we constructed an infection system based on an agroinoculation method to evaluate their virus resistance.