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Cryo-SEM/EDX study of the uptake and the radial movement of minerals in the tree trunk

Research Project

Project/Area Number 25292110
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Wood science
Research InstitutionForestry and Forest Products Research Institute

Principal Investigator

Kuroda Katsushi  国立研究開発法人 森林総合研究所, 木材特性研究領域, 主任研究員 (90399379)

Co-Investigator(Renkei-kenkyūsha) Itoh Yuko  国立研究開発法人森林総合研究所, 立地環境研究領域, 主任研究員 (60353588)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywordsクライオ / SEM / EDX / ミネラル / セシウム / 樹幹 / 放射柔細胞 / ICP-MS / 柔細胞 / スギ
Outline of Final Research Achievements

Ray parenchyma cells are a proposed pathway for the radial movement of some elements from the sapwood to the heartwood in the tree trunk. However, this function has not been demonstrated. In order to reveal the radial pathway of minerals in the tree trunk, a solution of the stable isotope cesium (Cs) was injected into the outer sapwood of Japanese cedar trees, and its radial movement through the xylem was traced at the cellular level using cryo-scanning electron microscopy/energy-dispersive X-ray spectroscopy (cryo-SEM/EDX) and inductively coupled plasma mass spectrometry (ICP-MS). Our findings provide the experimental facts that Cs is actively moved radially from sapwood toward intermediate wood through the ray parenchyma cells.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (9 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results) Book (1 results)

  • [Journal Article] Functions of Parenchyma Cells in Wood; Material Transport and Heartwood Formation2015

    • Author(s)
      黒田克史
    • Journal Title

      Mokuzai Gakkaishi

      Volume: 61 Issue: 3 Pages: 131-135

    • DOI

      10.2488/jwrs.61.131

    • NAID

      130005071856

    • ISSN
      0021-4795, 1880-7577
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The accumulation pattern of ferruginol in the heartwood-forming Cryptomeria japonica xylem as determined by time-of-flight secondary ion mass spectrometry and quantity analysis2014

    • Author(s)
      Katsushi Kuroda, Takeshi Fujiwara, Koh Hashida, Takanori Imai, Masayoshi Kushi, Kaori Saito, Kazuhiko Fukushima
    • Journal Title

      Annals of Botany

      Volume: 113 Issue: 6 Pages: 1029-1036

    • DOI

      10.1093/aob/mcu028

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal and clonal variation in cellulose microfibril orientation during cell wall formation of tracheids in Cryptomeria japonica2014

    • Author(s)
      Tuula Jyske, Takeshi Fujiwara, Katsushi Kuroda, Taiichi Iki, Chunhua Zhang, Tuomas K. Jyske, Hisashi Abe
    • Journal Title

      Tree Physiology

      Volume: - Issue: 8 Pages: 856-868

    • DOI

      10.1093/treephys/tpu008

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] クライオSEM/EDXによるスギ辺材に注入したセシウムの木部内放射方向の局在解析2016

    • Author(s)
      黒田克史、山根健一、伊藤優子
    • Organizer
      第66回日本木材学会大会
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] スギ樹幹に注入したセシウムのクライオSEM/EDXによる分布解析の試み2015

    • Author(s)
      黒田克史、山根健一、伊藤優子
    • Organizer
      第65回日本木材学会大会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Phloem structure and stilbene chemistry in Picea abies as revealed by novel microtechniques2015

    • Author(s)
      Jyske, T., Kuroda, K. et al.
    • Organizer
      IAWPS2015
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2015-03-16 – 2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Stilbenes in Norway spruce: variability and localization within phloem and bark2014

    • Author(s)
      Jyske, T., Kuroda, K. et al.
    • Organizer
      第27回国際ポリフェノール会議2014
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2014-09-02 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 心材形成過程の解析を目指したインピーダンス法によるカラマツ木部の水分布季節変化の非破壊計測2014

    • Author(s)
      黒田克史、鈴木養樹、中田了五
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      松山
    • Related Report
      2013 Annual Research Report
  • [Book] 植物細胞壁実験法(石井忠他編)2016

    • Author(s)
      黒田克史
    • Total Pages
      404
    • Publisher
      弘前大学出版会
    • Related Report
      2015 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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