| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Outline of Final Research Achievements |
Japanese flounder is an economically important aquaculture speceis in Asian countries such as Japan, Korea and China. Lymphocystis disease (LD) is one of important disease and causes serious damage to Japanese flounder aquaculture industry.
In this study, we investigated the candidate genes for Lymphocystis disease resistance in Japanese flounder using linkage analysis, gene expression analysis and sequence analysis. The genetic locus controlling the resistance to lymphocystis disease was localized on 1.1cM (about 134kb) by linkage analysis. Eleven cDNA of candidate genes from the locus controlling the resistance to lymphocystis disease were isolated and used for gene expression analysis and sequence analysis.
Two candidate genes for lymphocystis disease resistance were identified by gene expression analysis and sequence analysis. The genetic frequency of SNP on the candidate genes was about 2% in wild population.
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