Identification of the candidate genes for Lymphocystis disease resistance in Japanese flounder.

• Project/Area Number: 25292118
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Sakamoto Takashi 東京海洋大学, その他部局等, 教授 (40313390)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: ウイルス / 耐病性 / 遺伝子 / ゲノム / 育種 / ヒラメ / マーカー選抜 / SNP / マーカー選抜育種 / MAS

Outline of Final Research Achievements

Japanese flounder is an economically important aquaculture speceis in Asian countries such as Japan, Korea and China. Lymphocystis disease (LD) is one of important disease and causes serious damage to Japanese flounder aquaculture industry.
In this study, we investigated the candidate genes for Lymphocystis disease resistance in Japanese flounder using linkage analysis, gene expression analysis and sequence analysis. The genetic locus controlling the resistance to lymphocystis disease was localized on 1.1cM (about 134kb) by linkage analysis. Eleven cDNA of candidate genes from the locus controlling the resistance to lymphocystis disease were isolated and used for gene expression analysis and sequence analysis.
Two candidate genes for lymphocystis disease resistance were identified by gene expression analysis and sequence analysis. The genetic frequency of SNP on the candidate genes was about 2% in wild population.

Research Products

• [Int'l Joint Research] Yellow Sea Fisheries Research Institute(China)
• [Journal Article] Genome-Wide SNP Identification for the Construction of a High-Resolution Genetic Map of Japanese Flounder (Paralichthys olivaceous): applications to QTL Mapping of Vibrio anguillarum Disease Resistance and Comparative Genomic Analysis. (2015)
  • Author(s): C. Shao, Y. Niu, P. Rastas, Y. Liu, Z. Xie, H. Li, L. Wang, Y. Jiang, S. Tai, Y. Tian, T. Sakamoto and S. Chen.
  • Journal Title: DNA Research Volume: 22 (2) Issue: 2 Pages: 161-170
  • DOI: 10.1093/dnares/dsv001
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 養殖魚類における遺伝情報を活用したゲノム育種研究の現状と展望 (2016)
  • Author(s): 坂本崇
  • Organizer: 魚類防疫士連絡協議会 講演会
  • Place of Presentation: 東京都中央区
  • Year and Date: 2016-03-11
  • Invited
• [Presentation] ヒラメのリンホシスチス病抵抗性候補領域内に存在する遺伝子の単離と配列解析 (2015)
  • Author(s): 中嶋彩奈・法橋亜矢子・佐野元彦・坂本崇
  • Organizer: 日本水産学会
  • Place of Presentation: 宮城県仙台市（東北大学）
  • Year and Date: 2015-09-23
• [Presentation] CONVENIENT PHYSICAL GENOME MAP OF FLOUNDER. A PRACTICAL WHOLE- GENOME RADIATION HYBRID MAP OF JAPANESE FLOUNDER. (2015)
  • Author(s): Okamoto H., Aoki J., Nozaki T., Ozaki A., Uji S., Fuji K., Sakamoto T., Okamoto N., Kawai J., Araki K., Ekker M.
  • Organizer: THE INTERNATIONAL SYMPOSIUM ON GENETICS IN AQUACULTURE XII
  • Place of Presentation: SANTIAGO DE COMPOSTELA, SPAIN
  • Year and Date: 2015-06-22
  • Int'l Joint Research
• [Presentation] ヒラメリンホシスチス病抵抗性遺伝子座領域に存在する候補遺伝子の発現解析 (2014)
  • Author(s): 法橋亜矢子、杉森信哉、長谷川理、Changwei Shao、 Songlin Chen、佐野元彦、坂本崇
  • Organizer: 日本動物遺伝育種学会第15回大会
  • Place of Presentation: 埼玉県和光市（理化学研究所）
  • Year and Date: 2014-10-31 – 2014-11-01