Development of high perfomanced hydogen production system using marine vibrios as biocatalysts

• Project/Area Number: 25292122
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: Sawabe Tomoo 北海道大学, 水産科学研究科(研究院), 教授 (30241376)
• Co-Investigator(Kenkyū-buntansha): HOSOKAWA MASASHI 北海道大学, 大学院水産科学研究院, 准教授 (10241374) ; NAKAGAWA SATOSHI 北海道大学, 大学院水産科学研究院, 准教授 (70435832)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 再生可能エネルギー / 水素 / 水産学 / バイオマス / 微生物 / 生物工学 / カロテノイド / ゲノム

Outline of Final Research Achievements

Aims of the study are to establishment of 1) continuous culture system and 2) biorefinery of hydrogen producing marine vibrios as biocatalysts. We did succeeded 1) continuous hydrogen production for 70 days with appropriate hydrogen producing rates and yields, 2) alginate-hydrogen conversion using 2 kinds of biocatalysts, and 3) bioprospects of new and rare carotenoid producers and their genome information.

Research Products

• [Journal Article] Identification of a gene cluster responsible for hydrogen evolution in Vibrio tritonius strain AM2 with transcriptional analyses (2015)
  • Author(s): Y. Matsumura, H. Al-saari, S. Mino, S. Nakagawa, F. Maruyama, Y. Ogura, T. Hayashi, K. Kurolawa, T. Sawabe, T. Sawabe
  • Journal Title: Int. J. Hydrogen Energy Volume: 40 Issue: 30 Pages: 9137-9146
  • DOI: 10.1016/j.ijhydene.2015.05.137
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Draft Genome Sequences of Two Vibrionaceae Species, Vibrio ponticus C121 and Photobacterium aphoticum C119, Isolated as Coral Reef Microbiota (2014)
  • Author(s): N. Al-Saari, P.M. Meirelles, S. Mino, W. Suda, K. Oshima, M. Hattori, M. Ohkuma, F.L. Thompson, B. Gomez-Gil, T. Sawabe, T. Sawabe
  • Journal Title: Genome Announc. Volume: 2 Issue: 5
  • DOI: 10.1128/genomea.01095-14
  • Peer Reviewed / Open Access
• [Journal Article] Draft Genome Sequences of Marine Flavobacterium Nonlabens Strains NR17, NR24, NR27, NR32, NR33, and Ara13 (2014)
  • Author(s): M. Nakanishi, P. Meirelles, R. Suzuki, N. Takatani, S. Mino, W. Suda, K. Oshima, M. Hattori, M. Ohkuma, M. Hosokawa, K. Miyashita, F.L. Thompson, A. Niwa, T. Sawabe, T. Sawabe
  • Journal Title: Genome Announc. Volume: 2 Issue: 6
  • DOI: 10.1128/genomea.01165-14
  • Peer Reviewed / Open Access
• [Journal Article] Enhanced hydrogen production by a newly described heterotrophic marine bacterium, Vibrio tritonius strain AM2, using seaweed as the feedstock (2014)
  • Author(s): Y. Matsumura, K. Sato, N. Al-saari, S. Nakagawa, T. Sawabe
  • Journal Title: Int. J. Hydrogen Energy Volume: in press Issue: 14 Pages: 7270-7277
  • DOI: 10.1016/j.ijhydene.2014.02.164
  • Peer Reviewed
• [Journal Article] Updating the vibrio phylogeny defined by multilocus sequence phylogeny (2013)
  • Author(s): T. Sawabe, Y. Ogura, Y. Matsumura et al.
  • Journal Title: Front. Microbiol. Volume: 4 Pages: 414-414
  • DOI: 10.3389/fmicb.2013.00414
  • Peer Reviewed
• [Presentation] 水素生成マリンビブリオの網羅的遺伝子発現解析 (2015)
  • Author(s): 澤辺智雄、水越草太、美野さやか、松村佑太、澤辺桃子
  • Organizer: 第17回マリンバイオテクノロジー学会大会
  • Place of Presentation: 東京海洋大学（東京都、港区）
  • Year and Date: 2015-05-30
• [Presentation] 海洋細菌が有するカロテノイド合成系の体系的理解 (2014)
  • Author(s): 中西誠人・高谷直己・高峰・宮下和夫・細川雅史・大熊盛也・大島健志郎・服部正平・澤辺智雄
  • Organizer: 日本水産学会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2014-09-14 – 2014-09-17
• [Presentation] マリンビブリオを生物触媒としたギ酸塩の水素変換条件の最適化 (2014)
  • Author(s): 天田愛梨、澤辺智雄
  • Organizer: 日本生物工学会
  • Place of Presentation: コンベンションセンター（北海道札幌市)
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] 水素生成マリンビブリオの網羅的遺伝子発現解析 (2014)
  • Author(s): 水越草太、丸山史人、小椋義俊、林哲也、黒川顕、澤辺智雄
  • Organizer: 日本生物工学会
  • Place of Presentation: コンベンションセンター（北海道札幌市）
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] Hydrogen production potential of vibrios: the efficiency, genome structure, and expression (2014)
  • Author(s): T. Sawabe
  • Organizer: Vibrio2014
  • Place of Presentation: スターリング大学（スコットランド・エディンバラ）
  • Year and Date: 2014-04-01 – 2014-04-04
• [Presentation] Vibrio tritonius AM2Tを用いた海藻糖質からの水素生産システムの構築 (2013)
  • Author(s): 佐藤一道・天田愛梨・中川聡・澤辺智雄
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島国際会議場（広島県広島市）
• [Presentation] 海洋細菌Jejuia pallidilutea A1株の新規カロテノイド合成とストレス応答 (2013)
  • Author(s): 高谷直己・西田健太郎・澤辺智雄・宮下和夫・細川雅史
  • Organizer: 日本マリンバイテクノロジー学会
  • Place of Presentation: 沖縄県市町村自治会館（沖縄県那覇市）
• [Presentation] The development of hydrogen producing system from seaweed carbohydrate using marine Vibrio (2013)
  • Author(s): K. Sato，E. Amada, S. Nakagawa, T. Sawabe
  • Organizer: Asia BioHyLink 2013
  • Place of Presentation: 大阪市立大学（大阪府大阪市）
• [Presentation] Can the marine vibrio make bioconversion of formate to biohydrogen? (2013)
  • Author(s): E. Amda, K. Sato, S. Nakagawa, T. Sawabe
  • Organizer: Asia BioHyLink 2013
  • Place of Presentation: 大阪市立大学（大阪府大阪市）
• [Book] Springer Handbook of Marine Biotechnology (2015)
  • Author(s): T.A. Bruce, A. Gonzales, Y. Matsumura, F.L. Thompson, T. Sawabe
  • Total Pages: 13
  • Publisher: Springer