Studies on developmental stage specific gene expression regulation and metacyclogenesis in African trypanosomes

• Project/Area Number: 25292167
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: Inoue Noboru 帯広畜産大学, 原虫病研究センター, 教授 (10271751)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
• Keywords: アフリカトリパノソーマ / ライフサイクル / 細胞分化 / 遺伝子 / 転写翻訳制御 / ツェツェバエ

Outline of Final Research Achievements

In this research project, we have investigated in detail mechanisms of stage-specific gene expression regulation in African trypanosomes, which cause animal trypanosomoses, such as animal African trypanosomoses, Surra and Dourine. As a result, we have found that gene expression of congolense epimastigote specific protein (cesp) was regulated by cis-elements located in the 3UTR. In addition we found a novel epimastigote stage-specific hemoglobin receptor (TcEpHbR), which could be a novel target gene for development of trypanocidal drugs and vaccines against animal trypanosomoses.

Research Products

• [Int'l Joint Research] アイオワ大学/イェール大学(米国)
• [Int'l Joint Research] ヴィクトリア大学(カナダ)
• [Journal Article] Characterization of an epimastigote-stage-specific hemoglobin receptor of Trypanosoma congolense (2016)
  • Author(s): Yamasaki, S., Suganuma, K., Yamagishi, J., Asada, M., Yokoyama, N., Kawazu, S., Inoue, N.
  • Journal Title: Parasites & Vectors Volume: in press Issue: 1 Pages: 299-299
  • DOI: 10.1186/s13071-016-1563-9
  • NAID: 120006390942
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Studies of trypanosomiasis in the Luangwa valley, north-eastern Zambia (2015)
  • Author(s): Laohasinnarong D, Goto Y, Asada M, Nakao R, Hayashida K, Kajino K, Kawazu S, Sugimoto C, Inoue N, Namangala B
  • Journal Title: Parasit Vectors Volume: 8 Issue: 1 Pages: 497-497
  • DOI: 10.1186/s13071-015-1112-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa (2015)
  • Author(s): Nguyen, T. T., Motsiri, M. S., Taioe, M. O., Mtshali, M. S., Goto, Y., Kawazu, S., Thekisoe, O. M. M., Inoue, N.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 2 Pages: 217-220
  • DOI: 10.1292/jvms.14-0330
  • NAID: 130004700262
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A TeGM6-4r antigen-based immunochromatographic test (ICT) for animal trypanosomosis (2015)
  • Author(s): Nguyen, T. T., Ruttayaporn, N., Goto, Y., Kawazu, S., Sakurai, T., Inoue, N.
  • Journal Title: Parasitology Research Volume: 114 Issue: 11 Pages: 4319-4325
  • DOI: 10.1007/s00436-015-4672-z
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] アフリカ睡眠病の疫学 (2015)
  • Author(s): 井上 昇
  • Journal Title: 医学のあゆみ Volume: 253 Pages: 124-129
• [Journal Article] アフリカトリパノソーマ症対策の現在、そして未来 (2015)
  • Author(s): 菅沼啓輔、井上 昇
  • Journal Title: 生体の科学 Volume: 66 Pages: 339-342
• [Journal Article] Establishment of ATP-Based Luciferase Viability Assay in 96-Well Plate for <i>Trypanosoma congolense</i> (2014)
  • Author(s): Suganuma K, Allamanda P, Hakimi H, Zhou M, Angeles J. M., Kawazu S and Inoue N
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 11 Pages: 1437-1441
  • DOI: 10.1292/jvms.14-0273
  • NAID: 130004704128
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Genome Sequence of the Tsetse Fly (Glossina morsitans): Vector of African Trypanosomiasis (2014)
  • Author(s): Watanabe et al.
  • Journal Title: Science Volume: 344 Issue: 6182 Pages: 380-386
  • DOI: 10.1126/science.1249656
  • Peer Reviewed
• [Journal Article] Identification and Characterization of a <i>Trypanosoma congolense</i> 46 kDa Protein as a Candidate Serodiagnostic Antigen (2014)
  • Author(s): Zhou, M., Suganuma, K., Ruttayaporn, N., Nguyen, T.T., Yamasaki, S., Igarashi, I., Kawazu, S., Suzuki, Y., Inoue, N.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 6 Pages: 799-806
  • DOI: 10.1292/jvms.13-0462
  • NAID: 130003391280
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Adenosine-uridine-rich element is one of the required cis-elements for epimastigote form stage-specific gene expression of the congolense epimastigote specific protein (2013)
  • Author(s): Suganuma, K., Mochabo, K. M., Hakimi, H., Yamasaki, S., Yamagishi, J., Asada, M., Kawazu, S. and Inoue, N.
  • Journal Title: Molecular and Biochemical Parasitology Volume: 191 Issue: 1 Pages: 36-43
  • DOI: 10.1016/j.molbiopara.2013.09.001
  • Peer Reviewed
• [Journal Article] Expression, immunolocalization and serodiagnostic value of Tc38630 protein from Trypanosoma congolense (2013)
  • Author(s): Mochabo, K. M., Zhou, M., Suganuma, K., Kawazu, S., Suzuki, Y. and Inoue, N.
  • Journal Title: Parasitology Research Volume: 112 Issue: 9 Pages: 3357-3363
  • DOI: 10.1007/s00436-013-3515-z
  • Peer Reviewed
• [Presentation] ミコフェノール酸およびその誘導体の抗トリパノソーマ活性評価 (2015)
  • Author(s): 菅沼啓輔
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 杏林大学三鷹キャンパス（東京）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Trypanosoma congolenseにおけるヘモグロビン取込みに関する研究 (2015)
  • Author(s): 山崎詩乃
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 杏林大学三鷹キャンパス（東京）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Expression regulation mechanisms of epimastigote stage-specific genes in African trypanosome (2014)
  • Author(s): Keisuke Suganuma
  • Organizer: 18th Japanese-German Cooperative Symposium on Protozoan Diseases
  • Place of Presentation: ドレスデン工科大学（ドイツ）
  • Year and Date: 2014-09-30 – 2014-10-03
• [Presentation] Analysis of the hemoglobin uptake in Trypanosoma congolense (2014)
  • Author(s): Shino Yamasaki
  • Organizer: 18th Japanese-German Cooperative Symposium on Protozoan Diseases
  • Place of Presentation: ドレスデン工科大学（ドイツ）
  • Year and Date: 2014-09-30 – 2014-10-03
• [Presentation] ルシフェラーゼを利用したT. congolense HTS系の確立と道産木材樹皮からの新規抗トリパノソーマ化合物探索 (2014)
  • Author(s): 菅沼啓輔
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Trypanosoma congolenseはベクター内ステージでヘモグロビンを取り込む (2014)
  • Author(s): 山崎詩乃
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Identification and characterization of an RNA binding protein, Trypanosoma congolense uridine binding protein-1 (2014)
  • Author(s): Suganuma, K.
  • Organizer: 6th ASEAN Congress of Tropical Medicine and Parasitology
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Presentation] Expression Regulation Mechanisms of Epimastigote Stage-Specific Genes in African Trypanosome (2014)
  • Author(s): Inoue, N.
  • Organizer: 6th ASEAN Congress of Tropical Medicine and Parasitology
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Invited
• [Presentation] Trypanosoma congolense EMF特異的発現遺伝子cespの発現調節機構 (2014)
  • Author(s): 菅沼啓輔
  • Organizer: 第８３回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス（愛媛県松山市）
• [Presentation] Analysis of the cis-element for gene expression regulation in congolense epimastigote specific protein (CESP) (2013)
  • Author(s): Suganuma, K.
  • Organizer: 24th International Conference of the World Association for the Advanced of Veterinary Parasitology
  • Place of Presentation: Perth, Australia