Molecular mechanism of host-transition in vecotr-borne pathogens

• Project/Area Number: 25292168
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: FUKUMOTO SHINYA 帯広畜産大学, 原虫病研究センター, 准教授 (50376422)
• Co-Investigator(Kenkyū-buntansha): 井上 昇 帯広畜産大学, 原虫病研究センター, 教授 (10271751)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: ベクター / マラリア

Outline of Final Research Achievements

Vector-borne pathogens such as malaria parasite or filaria transit between biologically different hosts of mammals and arthropods. Host path conversion is an important event for pathogens and is essential for adapting to the parasitic environment and establishing transmission. Therefore, in this study, we aimed to clarify the host transformation adaptation mechanism in infection establishment. As a result of analyzing host transformation related genes identified by the applicants, it was revealed that the two host transformation points of the vector infectious stage and the host infectivity stage are controlled by the same gene. We also screened novel host transformation related genes and developed methods for producing genetically modified protozoa.

Research Products

• [Journal Article] Development of a Plasmodium berghei transgenic parasite expressing the full-length Plasmodium vivax circumsporozoite VK247 protein for testing vaccine efficacy in a murine model (2016)
  • Author(s): Masanori Mizutani, Shinya Fukumoto, Adam Patrice Soubeiga, Akira Soga, Mitsuhiro Iyori Shigeto Yoshida
  • Journal Title: Malaria J Volume: 15 Issue: 1 Pages: 251-258
  • DOI: 10.1186/s12936-016-1297-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of thioredoxin peroxidase-1 gene disruption on the liver stages of the rodent malaria parasite Plasmodium berghei. (2015)
  • Author(s): Usui M, Masuda-Suganuma H, Fukumoto S, Angeles JM, Hakimi H, Inoue N, Kawazu S.
  • Journal Title: Parasitol Int. Volume: Volume 64, Issue 3 Issue: 3 Pages: 290-294
  • DOI: 10.1016/j.parint.2014.09.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Serological survey of Toxoplasma gondii in wild sika deer in eastern Hokkaido, Japan (2015)
  • Author(s): Bando, H. Yoshimura, A. Koketsu, M. Soga, A. Taniguchi, Y. Ozaki, M. Suzuki, H. Kanuka, H. Fukumoto, S.
  • Journal Title: J Protozool Res Volume: 25 Pages: 48-52
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Detection of G119S ace-1 (R) mutation in field-collected Anopheles gambiae mosquitoes using allele-specific loop-mediated isothermal amplification (AS-LAMP) method (2015)
  • Author(s): Badolo, A. Bando, H. Traore, A. Ko-Ketsu, M. Guelbeogo, W. M. Kanuka, H. Ranson, H. Sagnon, N. Fukumoto, S.
  • Journal Title: Malar J Volume: 14 Issue: 1 Pages: 477-477
  • DOI: 10.1186/s12936-015-0968-9
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phylogenetic comparison of avian haemosporidian parasites from resident and migratory birds in northern Japan (2014)
  • Author(s): Yoshimura, A. Koketsu, M. Bando, H. Saiki, E. Suzuki, M. Watanabe, Y. Kanuka, H. Fukumoto, S.
  • Journal Title: J Wildl Dis Volume: 50 Issue: 2 Pages: 235-42
  • DOI: 10.7589/2013-03-071
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Expression analysis of autophagy-related genes in the hard tick Haemaphysalis longicornis. (2014)
  • Author(s): Umemiya-Shirafuji R, Galay RL, Maeda H, Kawano S, Tanaka T, Fukumoto S, Suzuki H, Tsuji N, Fujisaki K.
  • Journal Title: Vet. Parasitol. Volume: 201 Issue: 1-2 Pages: 169-175
  • DOI: 10.1016/j.vetpar.2014.01.024
  • Peer Reviewed
• [Journal Article] Baculovirus-Vectored Multistage Plasmodium vivax Vaccine Induces Both Protective and Transmission-Blocking Immunities against Transgenic Rodent Malaria Parasites (2014)
  • Author(s): Mizutani, M. Iyori, M. Blagborough, A. M. Fukumoto, S. Funatsu, T. Sinden, R. E. Yoshida, S.
  • Journal Title: Infect Immun Volume: 82 Issue: 10 Pages: 4348-4357
  • DOI: 10.1128/iai.02040-14
  • Peer Reviewed / Open Access
• [Journal Article] Intra-specific diversity of Serratia marcescens in Anopheles mosquito midgut defines Plasmodium transmission capacity (2013)
  • Author(s): Bando, H. Okado, K. Guelbeogo, W. M. Badolo, A. Aonuma, H. Nelson, B. Fukumoto, S. Xuan, X. Sagnon, N. Kanuka, H.
  • Journal Title: Sci Rep Volume: 3 Issue: 1 Pages: 1641-1641
  • DOI: 10.1038/srep01641
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Activation of Imd pathway in hemocyte confers infection resistance through humoral response in Drosophila. (2013)
  • Author(s): Bryce Nelson, et al.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 430 Issue: 3 Pages: 1120-1125
  • DOI: 10.1016/j.bbrc.2012.12.027
  • Peer Reviewed
• [Journal Article] Expression profiles of peroxiredoxins in liver stage of the rodent malari a parasite Plasmodium berghei (2013)
  • Author(s): Usui M, Masuda-Suganuma H, Fukumoto S, Angeles JM, Inoue N, Kawazu S.
  • Journal Title: Parasitology International Volume: Volume 62 Issue: 3 Pages: 337-340
  • DOI: 10.1016/j.parint.2012.11.007
  • Peer Reviewed
• [Journal Article] Multivariate analysis of host amino acids in plasma and liver during infection of malaria parasite Plasmodium yoelii (2012)
  • Author(s): Saiki E, Nagao K, Aonuma ll, Fukumoto S. Xuan X, Bannai M, Kanuka H
  • Journal Title: Malaria Journal Volume: 12 Issue: 1 Pages: 19-19
  • DOI: 10.1186/1475-2875-12-19
  • Peer Reviewed
• [Presentation] Filarial nematode Dirofilaria immitis diverts thermoregulation to developmental transition following transmission by Aedes mosquito (2017)
  • Author(s): S. Fukumoto, A. Yoshimura, H. Kanuka
  • Organizer: Molecular Helminthology: An Integrated Approach Conference 2017.
  • Place of Presentation: Hyannis (USA)
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] 哺乳類宿主毒性抗生剤を利用したPlasmodium berghei多重変異体作製技術の開発 (2016)
  • Author(s): 曽賀晃、纐纈摩美、福本晋也
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-11-30
• [Presentation] In vitro哺乳動物毒性マーカーシステムの応用によるマウスマラリアモデル 多重変異体選択技術の開発 (2016)
  • Author(s): 曽賀晃、纐纈摩美、福本晋也
  • Organizer: 第159回日本獣医学会
  • Place of Presentation: 日本大学（神奈川県・藤沢市）
  • Year and Date: 2016-09-06
• [Presentation] アミノ酸摂取量の調整によるマラリア制御の可能性 (2016)
  • Author(s): 齊木 選射、長尾 健児、石上 盛敏、福本 晋也、クルドゥスッドゥ スリヴィッチャ、 櫻井 達也、坂内 慎、狩野 繁之、嘉糠 洋陸
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎県宮崎市、宮崎市民プラザ
  • Year and Date: 2016-03-19
• [Presentation] In vitro-puromycinシステムによる高効率遺伝子組換えPlasmodium berghei選択法の開発 (2015)
  • Author(s): 曽賀晃、纐纈摩美、福本晋也
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市、神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] Comparison of LAMP and RT-PCR methods for Plasmodium faclciparum detection in field collected Anopheles gambiae mosquitoes (2015)
  • Author(s): Athanase Badolo, Moussa W. Guelbeogo, N’Falé Sagnon, Shiniya Fukumoto and Hirotaka Kanuka
  • Organizer: EMBO Conference on Molecular and Population Biology of Mosquitoes and Other Disease Vectors: Current, Resurgent and Emerging Diseases
  • Place of Presentation: Kolymbari, Greece
  • Year and Date: 2015-07-24
  • Int'l Joint Research
• [Presentation] LAMP法によるG119S Ace1R変異検出法の開発と西アフリカ-ブルキナファソ採集ガンビアハマダラカでの検証 (2015)
  • Author(s): アタナセ バドロ、纐纈摩美、福本晋也
  • Organizer: 第67回日本衛生動物学会
  • Place of Presentation: 石川県金沢市、金沢大学
  • Year and Date: 2015-03-27
• [Presentation] フィールド採集ハマダラカからの等温遺伝子増幅法による熱帯熱マラリア原虫検出法の検討 (2015)
  • Author(s): 曽賀晃、アタナセ バドロ、纐纈摩美、福本晋也
  • Organizer: 第67回日本衛生動物学会
  • Place of Presentation: 石川県金沢市、金沢大学
  • Year and Date: 2015-03-27
• [Presentation] 熱帯熱マラリアにおける重症化と宿主血中アミノ酸群動態の相関 (2015)
  • Author(s): 齋木選射 長尾健児 石上盛敏 福本晋也 クルドゥスッドゥスリヴィッチャ 櫻井達也 坂内慎 狩野繁之 嘉糠洋陸
  • Organizer: 第84回日本寄生虫学会
  • Place of Presentation: 東京都三鷹市、杏林大学
  • Year and Date: 2015-03-21
• [Presentation] ピューロマイシン耐性遺伝子を用いたマウスマラリア原虫新規変異体作製法の検討 (2014)
  • Author(s): 曽賀晃 纐纈摩美 福本晋也
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道札幌市、北海道大学
  • Year and Date: 2014-09-09
• [Presentation] Development of LAMP-based method to detect 1014F kdr and 119S ace-1R mutations in field collected Anopheles gambiae s.l. (2013)
  • Author(s): A. Badolo A., Fukumoto S., Okado K., Aonuma H., Guelbeogo W.M., Bando H., Yoshimura A., Sagnon N’F and Kanuka H.
  • Organizer: EMBO conference on Molecular and Population Biology of Mosquitoes and Other Disease Vectors: From Basic Vector Biology to Disease Control
  • Place of Presentation: Orthodox Academy Of Crete, Crete, Greece.
• [Presentation] 西アフリカに生息するマラリア媒介蚊の腸内細菌種内多様性に関する研究 (2013)
  • Author(s): 伴戸寛徳、岡戸清、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 第156回日本獣医学会
  • Place of Presentation: 岐阜大学（ 岐阜県岐阜市 ）
• [Presentation] Development of LAMP-based method to detect 1014F kdr and 119S ace-1R mutations in field collected Anopheles gambiae s.l. (2013)
  • Author(s): A. Badolo A., Fukumoto S., Okado K., Aonuma H., Guelbeogo W.M., Bando H., Yoshimura A., Sagnon N’F and Kanuka H.
  • Organizer: 6th MIM Pan-African Malaria Conference.
  • Place of Presentation: The International Convention Centre, Durban, South Africa
• [Presentation] マラリアをモデルとした重症化と宿主血中アミノ酸ダイナミクスの相関解析 (2013)
  • Author(s): 齊木 選射，青沼 宏佳，長尾 健児，福本 晋也，坂内 慎，嘉糠 洋陸
  • Organizer: 第36 回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド（ 兵庫県神戸市 ）