Mechanisms for the navigation of the intracellular transport

• Project/Area Number: 25293046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Okada Yasushi 国立研究開発法人理化学研究所, 生命システム研究センター, チームリーダー (50272430)
• Co-Investigator(Kenkyū-buntansha): Takai Akira 国立研究開発法人理化学研究所, 生命システム研究センター, 基礎科学特別研究員 (60637205) ; Shima Tomohiro 独立行政法人理化学研究所, 生命システム研究センター, 基礎科学特別研究員 (60631786) ; Ikeda Kazuho 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (20642565) ; Ito Yoko 国立研究開発法人理化学研究所, 生命システム研究センター, 特別研究員 (60584571)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: 細胞内輸送 / 細胞極性 / 微小管 / 分子モーター / キネシン / 微小管結合タンパク質

Outline of Final Research Achievements

The navigation mechanism for the intracellular transport has remained unclear. We are proposing a model that the conformational differences of micorubules between the GTP-bound and GDP-bound state would serve as the directional cue for kinesins that drive the intracellular transport into the neuronal axons. In this study, we have clarified the underlying mechanisms for this transport by the combination of single molecule imaging and the structural analyses.

Research Products

• [Journal Article] The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle. (2015)
  • Author(s): Chinen T, Liu P, Shioda S, Pagel J, Cerikan B, Lin TC, Gruss O, Hayashi Y, Takeno H, Shima T, Okada Y, Hayakawa I, Hayashi Y, Kigoshi H, Usui T, Schiebel E
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 8722-8722
  • DOI: 10.1038/ncomms9722
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics (2015)
  • Author(s): Shinichi Hayashi and Yasushi Okada
  • Journal Title: Molecular Biology of the Cell Volume: 26 Issue: 9 Pages: 1743-1751
  • DOI: 10.1091/mbc.e14-08-1287
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expanded palette of Nano-lantern for real-time muliti-color luminescence imaging (2015)
  • Author(s): Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin T, Okada Y, Nagai T
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 112 Issue: 14 Pages: 4352-4356
  • DOI: 10.1073/pnas.1418468112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Compact and stable SNAP ligand-conjugated quantum dots as a fluorescent probe for single-molecule imaging of dynein motor protein. (2015)
  • Author(s): Ohyanagi T, Shima T, Okada Y, Tsukasaki Y, Komatsuzaki A, Tsuboi S, Jin T.
  • Journal Title: Chemical Communications Volume: 51 Issue: 80 Pages: 14836-14839
  • DOI: 10.1039/c5cc05526a
  • Peer Reviewed / Open Access
• [Journal Article] Super-resolution imaging of nuclear bodies by STED microscopy. (2015)
  • Author(s): Okada Y, Nakagawa S,
  • Journal Title: Meth Mol Biol Volume: 1262 Pages: 21-35
  • DOI: 10.1007/978-1-4939-2253-6_2
  • ISBN: 9781493922529, 9781493922536
  • Peer Reviewed
• [Journal Article] Quantitative analysis of APP axonal transport in neurons: role of JIP1 in enhanced APP anterograde transport. (2014)
  • Author(s): Chiba K, Araseki M, Nozawa K, Furukori K, Araki Y, Matsushima T, Nakaya T,Hata S, Saito Y, Uchida S, Okada Y, Nairn AC, Davis RJ, Yamamoto T, Kinjo M, Taru, H, Suzuki T.
  • Journal Title: Mol Biol Cell Volume: 25 Issue: 22 Pages: 3569-3580
  • DOI: 10.1091/mbc.e14-06-1111
  • NAID: 120005522339
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of microtubule-based transport by MAP4. (2014)
  • Author(s): Semenova I, Ikeda K, Resaul K, Kraikivski P, Aguiar M, Gygi S, Zaliapin I, Cowan A, Rodionov V.
  • Journal Title: Molecular Biology of the Cell Volume: 25 Issue: 20 Pages: 3119-32
  • DOI: 10.1091/mbc.e14-01-0022
  • Peer Reviewed / Open Access
• [Journal Article] A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging (2014)
  • Author(s): Shin-nosuke Uno, Mako Kamiya, Toshitada Yoshihara, Ko Sugawara, Kohki Okabe, Mehmet C. Tarhan, Hiroyuki Fujita, Takashi Funatsu, Yasushi Okada, Seiji Tobita, Yasuteru Urano
  • Journal Title: Nature Chemistry Volume: 6 Issue: 8 Pages: 681-689
  • DOI: 10.1038/nchem.2002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Viscosity and drag force involved in organelle transport : Investigation of the fluctuation dissipation theorem (2013)
  • Author(s): K. Hayashi, C.G. Pack, M. K. Sato, K. Mouri, K. Kaizu, K. Takahashi and Y. Okada
  • Journal Title: Euro Phys J E Volume: 36 Issue: 12 Pages: 136-136
  • DOI: 10.1140/epje/i2013-13136-6
  • Peer Reviewed
• [Presentation] 細胞の中で分子モーターが迷子にならない機構 (2016)
  • Author(s): 岡田康志
  • Organizer: 膜生物学・医学学術講演会
  • Place of Presentation: 神戸大学医学部(兵庫県、神戸市）
  • Year and Date: 2016-02-26
  • Invited
• [Presentation] 量子脳仮説は荒唐無稽なのか (2016)
  • Author(s): 岡田康志
  • Organizer: 第１回量子と生命研究会
  • Place of Presentation: 阪大Σホール（大阪府、豊中市）
  • Year and Date: 2016-01-29
  • Invited
• [Presentation] Cooperative conformational changes of microtubule by kinesin as the mechanism for the spontaneous polarization of the intracellular transport in neurons (2015)
  • Author(s): 岡田康志
  • Organizer: Pacifichem 2015
  • Place of Presentation: ホノルル(米国）
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] 一分子イメージングによる細胞質内での反応速度定数の直接計測 (2015)
  • Author(s): 岡田康志
  • Organizer: 日本分子生物学会・日本生化学会合同大会
  • Place of Presentation: 神戸国際会議場（兵庫県、神戸市）
  • Year and Date: 2015-12-02
  • Invited
• [Presentation] Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics (2015)
  • Author(s): 岡田康志
  • Organizer: The 2nd East Asia Microscopy Conference
  • Place of Presentation: 姫路商工会議所（兵庫県、姫路市）
  • Year and Date: 2015-11-25
  • Int'l Joint Research / Invited
• [Presentation] 細胞極性制御における自発的対称性の破れと秩序形成 (2015)
  • Author(s): 岡田康志
  • Organizer: 山田研究会「生物と非生物をつなぐ」
  • Place of Presentation: ラフォーレ修善寺（静岡県、伊豆市）
  • Year and Date: 2015-11-17
  • Invited
• [Presentation] ライブセルイメージングのための高速超解像蛍光顕微鏡の開発 (2015)
  • Author(s): 岡田康志
  • Organizer: 日本光学会年次学術講演会シンポジウム
  • Place of Presentation: 筑波大学（東京都、文京区）
  • Year and Date: 2015-10-28
  • Invited
• [Presentation] 超解像蛍光ライブセルイメージングの現状と課題 (2015)
  • Author(s): 岡田康志
  • Organizer: 日本顕微鏡学会関西支部平成27年度特別講演会
  • Place of Presentation: 京都大学黄檗プラザ（京都府、宇治市）
  • Year and Date: 2015-10-03
  • Invited
• [Presentation] Conformational switching of tubulin serves as the guidance cue for the intracellular transport by kinesin (2015)
  • Author(s): 岡田康志
  • Organizer: 第53回日本生物物理学会年会シンポジウム
  • Place of Presentation: 金沢大学（石川県、金沢市）
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] スピニングディスク共焦点光学系を利用した高速超解像ライブイメージング (2015)
  • Author(s): 岡田康志
  • Organizer: 第67回細胞生物学会大会シンポジウム
  • Place of Presentation: タワーホール船堀（東京都、江戸川区）
  • Year and Date: 2015-07-02
  • Invited
• [Presentation] 1分子イメージングによる細胞質内での反応速度定数の直接計測 (2015)
  • Author(s): 岡田康志
  • Organizer: ナノメディシン分子科学公開シンポジウム
  • Place of Presentation: 東京大学（東京都、文京区）
  • Year and Date: 2015-07-01
  • Invited
• [Presentation] Dissecting kinesin regulation through single molecule in cellulo measurements (2015)
  • Author(s): 岡田康志
  • Organizer: Janelia Conference
  • Place of Presentation: アシュバーン（米国）
  • Year and Date: 2015-05-30
  • Int'l Joint Research / Invited
• [Presentation] 超解像・1分子イメージングで探る細胞内物質輸送の制御機構 (2015)
  • Author(s): 岡田康志
  • Organizer: 日本顕微鏡学会第71回学術講演会シンポジウム
  • Place of Presentation: 京都国際会館（京都府、京都市）
  • Year and Date: 2015-05-14
  • Invited
• [Presentation] Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics (2015)
  • Author(s): 岡田康志
  • Organizer: 9th Asian Biophysics Association Symposium
  • Place of Presentation: 杭州（中国）
  • Year and Date: 2015-05-09
  • Int'l Joint Research / Invited
• [Presentation] Atomic mechanism for the intracellular navigation of molecular motor, kinesin (2013)
  • Author(s): 岡田康志
  • Organizer: Joint Weizmann-MBI mechanobiology conference
  • Place of Presentation: シンガポール
  • Invited
• [Presentation] Imaging-based analyses of the intracellular navigation mechanism of molecular motor, kinesin (2013)
  • Author(s): 岡田康志
  • Organizer: 第３６回日本分子生物学会シンポジウム
  • Place of Presentation: 神戸
  • Invited
• [Presentation] 細胞内輸送のナビゲーション機構 (2013)
  • Author(s): 岡田康志
  • Organizer: 定量生物学の会第６回年会
  • Place of Presentation: 吹田
  • Invited
• [Presentation] Structural basis for the polarized axonal transport in neuron (2013)
  • Author(s): 岡田康志
  • Organizer: 第５１回日本生物物理学会
  • Place of Presentation: 京都
  • Invited
• [Presentation] 分子モーターが細胞内で迷わない機構 (2013)
  • Author(s): 岡田康志
  • Organizer: 日本顕微鏡学会第６９回学術講演会
  • Place of Presentation: 吹田
  • Invited
• [Presentation] Super-resolution live imaging of intracellular transport system
  • Author(s): 岡田康志
  • Organizer: Super Imaging 2013
  • Place of Presentation: 浜松
  • Invited
• [Remarks] 新しいタイプの抗がん剤開発につながる成果 ～γ－チューブリン特異的阻害剤の開発に成功～
  • URL: http://www.tsukuba.ac.jp/attention-research/p201510301400.html
• [Remarks] 肉眼でも観察できる！従来の20倍光るタンパク質を開発
  • URL: http://resou.osaka-u.ac.jp/ja/research/2015/20150324_1
• [Remarks] シャッター速度世界一の超解像蛍光顕微鏡を開発
  • URL: http://www.riken.jp/pr/press/2015/20150415_1/
• [Remarks] 研究最前線 キネシンは、なぜ迷子にならない？
  • URL: http://www.riken.jp/pr/blog/2015/151207_1/