Building genomic information platform of Oncomelania snails, intermediate host snails of Schistosoma japonicum.

• Project/Area Number: 25293099
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Ohta Nobuo 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (10143611)
• Co-Investigator(Kenkyū-buntansha): KUMAGAI Takashi 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (40369054) ; MARUYAMA Haruhiko 宮崎大学, 医学部, 教授 (90229625)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 日本住血吸虫 / Oncomelania / ゲノム解析 / 系統解析 / RNA-sec / ミヤイリガイ / ミトコンドリアゲノム / 共進化 / Oncomelania nosophora / 中間宿主 / ゲノム情報 / 次世代シーケンサー / ミトコンドリア / ゲノム / 株間変異 / 超ハイスループット遺伝子解析

Outline of Final Research Achievements

Genetic studies on the intermediate host snails, Oncomelania spp, was performed. By the use of genome-amplification method, whole genome analysis was possible from the limited number of snails, and the genome size was 1.47g base, which was much more bigger than Biomphalaria snails. Whole mitochondrial DNA was sequenced and phylogenetic analysis was done about five sub-species of Oncomelania hupensis. Results indicated that the origin of this snail species seemed to be O. h. robertsoni, distributed in the west part of China, and mover to the east to form O. h. hupensis and finally to O. h. nosophora distributed in Japan. The Filipino snails, O. h. quadrasi seemed have separated from O. h. robertosoni. Considering research on Schistosoma japonicum, evolutional profiles were different from their intermediate host snails.

Research Products

• [Int'l Joint Research] Zhejiang Institute of Parasitic Diseases/Anhui Institute of Parasitic Diseases(China)
• [Journal Article] A novel method to assess the biodiversity of parasites using 18S rDNA Illumina sequencing; parasitome analysis method. (2016)
  • Author(s): Hino A, Maruyama H, Kikuchi T.
  • Journal Title: Parasitology International Volume: 65
  • Peer Reviewed
• [Journal Article] Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase (SjGST) DNA vaccine against murine Schistosoma japonicum infection. (2015)
  • Author(s): Mbanefo EC, Kumagai T, Kodama Y, Kurosaki T, Furushima-Shimogawara R, Cherif MS, Mizukami S, Kikuchi M, Huy NT, Ohta N, Sasaki H, Hirayama K.
  • Journal Title: Parasitol Int. Volume: 64 Issue: 4 Pages: 24-31
  • DOI: 10.1016/j.parint.2015.01.005
  • Peer Reviewed / Open Access
• [Journal Article] A new surveillance and response tool: risk map of infected Oncomelania hupensis detected by Loop-mediated isothermal amplification (LAMP) from pooled samples. (2015)
  • Author(s): Tong QB, Chen R, Zhang Y, Yang GJ, Kumagai T, Furushima-Shimogawara R, Lou D, Yang K, Wen LY, Lu SH, Ohta N, Zhou XN.
  • Journal Title: Acta Tropica Volume: 141 Pages: 170-177
  • Peer Reviewed
• [Journal Article] A new surveillance and response tool: risk map of infected Oncomelania hupensis detected by Loop-mediated isothermal amplification (LAMP) from pooled samples. (2015)
  • Author(s): Tong QB, Chen R, Zhang Y, Yang GJ, Kumagai T, Furushima-Shimogawara R, Lou D, Yang K, Wen LY, Lu SH, Ohta N, Zhou XN.
  • Journal Title: Acta Trop. Volume: 141 Pages: 170-177
  • DOI: 10.1016/j.actatropica.2014.01.006
  • Peer Reviewed
• [Journal Article] Basophil depletion downregulates Schistosoma mansoni egg-induced granuloma formation (2013)
  • Author(s): Anyan, W.K., Seki, T., Kumagai, T., Obata-Ninomiya, K., Furushima-Shimogawara, R., Kwansa-Bentum, B., Akao, N., Bosompem, K.M., Boakye, D.A., Wilson, M.D., Karasuyama, H., and Ohta, N.
  • Journal Title: Parasitol. Int. Volume: 62 Issue: 6 Pages: 508-513
  • DOI: 10.1016/j.parint.2013.07.003
  • Peer Reviewed
• [Presentation] 日本住血吸虫中間宿主貝 Oncomelania hupensis亜種間の全ミトコンドリアゲノム配列を用いた系統解析 (2016)
  • Author(s): 日野明紀菜、菊地泰生、熊谷貴、齋藤康秀、下河原理江子、丸山治彦、太田伸生
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎県宮崎市、宮崎市民プラザ
  • Year and Date: 2016-03-19
• [Presentation] 日本住血吸虫ミラシジウムの侵入と発育における感染感受性から見たOncomelania spp.各亜種が示す防御機構の特徴 (2016)
  • Author(s): 小笠原啓祐、下河原理江子、熊谷貴、日野明紀菜、二瓶直子、齋藤康秀、太田伸生
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎県宮崎市、宮崎市民プラザ
  • Year and Date: 2016-03-19
• [Presentation] 日本住血吸虫の中間宿主貝Oncomelania hupensis spp.が示す侵入と発育に対する防御機構について (2015)
  • Author(s): 下河原理江子、熊谷貴、齋藤康秀、二瓶直子、Chen Rui、太田伸生
  • Organizer: 第26回日本生体防御学会学術総会
  • Place of Presentation: 東京都台東区、台東区生涯学習センター
  • Year and Date: 2015-07-11
• [Presentation] 中間宿主貝類 Oncomelania hupensis spp.におけるSchistosoma japonicumミラシジウムの感染性から見た防御機構について (2015)
  • Author(s): 下河原 理江子、熊谷 貴、齋藤康秀、二瓶 直子、太田伸生
  • Organizer: 第84回日本寄生虫学会
  • Place of Presentation: 東京都三鷹市（杏林大学）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Molecular approach for controlling schistosomiasis japonica through snail monitoring and evaluation. (2014)
  • Author(s): Ohta N, Kumagai T, Shimogawara-Furushika R, Seki T, Ishikawa H, Wang T, Chen R, Lu SH.
  • Organizer: International Congress of Parasitology (ICOPA) 2014.
  • Place of Presentation: Mexico City (Mexico)
  • Year and Date: 2014-08-10 – 2014-08-15
  • Invited
• [Presentation] Control of schistosomiasis japonica through snail monitoring and evaluation: Research in molecular approach
  • Author(s): Ohta N, Kumagai T, Shimogawara-Furushima R, Seki T, Ishikawa H, Wang T, Chen R, Lu S.
  • Organizer: ICOPA XII
  • Place of Presentation: Mexico City
  • Invited
• [Book] 寄生虫学研究 材料と方法 2014 (2014)
  • Author(s): 熊谷 貴、下河原 理江子、山邊 将史、太田伸生
  • Total Pages: 4
  • Publisher: 三恵社