| Project/Area Number |
25293127
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUI TAKANORI 久留米大, 医学部, 講師 (10425233)
UEDA SHIN-ICHIRO 久留米大, 医学部, 助教 (00412502)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | 糖尿病合併症治療 / DNAアプタマー / 核酸医薬品 / 終末糖化産物 / 終末糖化産物受容体 / 癌治療 / 糖尿病合併症 / AGE / アプタマー / RAGE / 酸化ストレス |
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| Outline of Final Research Achievements |
Advanced glycation end products (AGEs) and their receptor (RAGE) play a role in diabetes-associated complications, such as diabetic nephropathy and cancer. We screened DNA aptamer directed against RAGE (RAGE-aptamer) in vitro, and examined its effects on renal injury in streptozotocin-induced diabetic rats and melanoma growth in nude mice. Urinary albumin and 8-hydroxy-2’-deoxy-guanosine, AGEs and RAGE levels were increased, and enhanced glomerular extracellular matrix accumulation were observed in diabetic rats, all of which were prevented by RAGE-aptamer. Moreover, RAGE-aptamer inhibited tumor growth in nude mice, and decreased expression levels of proliferating nuclear antigen, CD31 and Mac-3, markers of endothelial cells and macrophages, respectively. RAGE-aptamer may be a novel therapeutic agent for diabetes-associated complications.
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