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Research of neutralizing antibody recognizing glycopeptides onto hepatitis C virus and basic studies for new diagnoses

Research Project

Project/Area Number 25293134
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Shimizu Hiroki  国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (30344716)

Co-Investigator(Kenkyū-buntansha) 奥田 徹哉  国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (20443179)
鈴木 哲朗  浜松医科大学, 医学部, 教授 (00250184)
松岡 浩司  埼玉大学, 理工学研究科, 教授 (40272281)
松尾 一郎  群馬大学, 大学院理工学府, 教授 (40342852)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
KeywordsC型肝炎 / 糖ペプチド / 糖鎖 / 抗体 / 糖 / C型肝炎
Outline of Final Research Achievements

The aim of this project was research of neutralizing antibody recognizing glycopeptides onto hepatitis C virus (HCV) and basic studies for new diagnoses.
We tried to produce a neutralizing antibody which recognize glycopeptide in E2 glycoprotein onto HCV. The first stage, to synthesize glycopeptide antigen having developed (by Okuda et al) immunization tag, faced many difficulties, but finally, we achieved to synthesize the designed glycopepitde antigen with aid of new microwave techniques which Shimizu et al developed. Then, immunization was performed and currently produced antibodies are evaluated.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (25 results)

All 2017 2016 2015 2014 2013 Other

All Journal Article (13 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 13 results,  Open Access: 9 results,  Acknowledgement Compliant: 6 results) Presentation (10 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Localized surface plasmon resonance-mediated fluorescence signals in plasmonic nanoparticle-quantum dot hybrids for ultrasensitive Zika virus RNA detection via hairpin hybridization assays.2017

    • Author(s)
      Adegoke O, Morita M, Kato T, Ito M, Suzuki T, Park EY.
    • Journal Title

      Biosens Bioelectron.

      Volume: 94 Pages: 513-522

    • DOI

      10.1016/j.bios.2017.03.046

    • NAID

      120006542499

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nonstructural 5A Protein of Hepatitis C Virus Interferes with Toll-Like Receptor Signaling and Suppresses the Interferon Response in Mouse Liver.2017

    • Author(s)
      Tsutsumi T, Okushin K, Enooku K, Fujinaga H, Moriya K, Yotsuyanagi H, Aizaki H, Suzuki T, Matsuura Y, Koike K.
    • Journal Title

      PLOS One

      Volume: 12 Issue: 1 Pages: 2017-2017

    • DOI

      10.1371/journal.pone.0170461

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synthetic Assembly of Mannose Moieties using Polymer Chemistry and the Biological Evaluation of its Interaction towards Concanavalin A2017

    • Author(s)
      D. Diwan, K. Shinkai, T. Tetsuka, B. Cao, H. Arai, T. Koyama, K. Hatano, and K. Matsuoka
    • Journal Title

      Molecules

      Volume: 22 Issue: 1 Pages: 157-157

    • DOI

      10.3390/molecules22010157

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biological Evaluation of Multivalent-Type N-Acetyl-D-Glucosamine (GlcNAc) Conjugates for Wheat Germ Agglutinin (WGA) by the Surface Plasmon Resonance (SPR) Method2017

    • Author(s)
      A. Kumari, T. Koyama, K. Hatano, and K. Matsuoka
    • Journal Title

      SOJ Biochem

      Volume: 2 Issue: 3 Pages: 7-7

    • DOI

      10.15226/2376-4589/2/3/00118

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] LUC7L3/CROP inhibits replication of hepatitis B virus via suppressing enhancer II/basal core promoter activity.2016

    • Author(s)
      Li Y, Ito M, Sun S, Chida T, Nakashima K, Suzuki T.
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1 Pages: 36741-36741

    • DOI

      10.1038/srep36741

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Synthetic Aassembly of Novel Avidin-biotin-GlcNAc (ABG) Complex as an Attractive Bio-probe and its Interaction with Wheat Germ Agglutinin (WGA)2016

    • Author(s)
      A. Kumari, T. Koyama, K. Hatano, and K. Matsuoka
    • Journal Title

      Bioorg. Chem.

      Volume: 68 Pages: 219-225

    • DOI

      10.1016/j.bioorg.2016.08.002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-domain Intrabodies against HCV Core Inhibit Viral Propagation and Core-induced NF-κB Activation2016

    • Author(s)
      Suzuki R, Saito K, Matsuda M, Sato M, Kanegae Y, Shi G, Watashi K, Aizaki H, Chiba J, Saito I, Wakita T, Suzuki T
    • Journal Title

      J Gen Virol

      Volume: 印刷中 Issue: 4 Pages: 887-892

    • DOI

      10.1099/jgv.0.000423

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Involvement of the 3' Untranslated Region in Encapsidation of the Hepatitis C Virus2016

    • Author(s)
      Shi G, Ando T, Suzuki R, Matsuda M, Nakashima K, Ito M, Omatsu T, Oba M, Ochiai H, Kato T, Mizutani T, Sawasaki T, Wakita T, Suzuki T.
    • Journal Title

      PLoS Pathog

      Volume: 12(2) Issue: 2 Pages: e1005441-e1005441

    • DOI

      10.1371/journal.ppat.1005441

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model2015

    • Author(s)
      Masayoshi Fukasawa, Shotaro Nagase, Yoshitaka Shirasago, Manami Iida, Mayo Yamashita, Kohki Endo, Kiyohito Yagi, Tetsuro Suzuki, Takaji Wakita, Kentaro Hanada, Hiroki Kuniyasu, Masuo Kondoh
    • Journal Title

      J Virol

      Volume: 89 Issue: 9 Pages: 4866-4879

    • DOI

      10.1128/jvi.03676-14

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Targeting cellular squalene synthase, an enzyme essential for cholesterol biosynthesis is a potential antiviral strategy against hepatitis C virus2015

    • Author(s)
      Kyoko Saito, Yoshitaka Shirasago, Tetsuro Suzuki, Hideki Aizaki, Kentaro Hanada, Takaji Wakita, Masahiro Nishijima, Masayoshi Fukasawa
    • Journal Title

      J. Virol.

      Volume: 89 Issue: 4 Pages: 2220-2232

    • DOI

      10.1128/jvi.03385-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Efficiency of 2.45 and 5.80 GHz microwave irradiation for a hydrolysis reaction by thermostable beta-Glucosidase HT12014

    • Author(s)
      Nagashima, Izuru; Sugiyama, Jun-ichi; Sakuta, Tomomi; Sasaki, Masahide; Shimizu, Hiroki
    • Journal Title

      Biosci. Biotechnol. Biochem.,

      Volume: 78 Issue: 5 Pages: 758-760

    • DOI

      10.1080/09168451.2014.891931

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Hepatitis C Virus NS5A Hyperphosphorylation Mediated by Casein Kinase I-α in Infectious Virus Production.2014

    • Author(s)
      Masaki T, Matsunaga S, Takahashi H, Nakashima K, Kimura Y, Ito M, Matsuda M, Murayama A, Kato T, Hirano H, Endo Y, Lemon SM, Wakita T, Sawasaki T, Suzuki T
    • Journal Title

      J Virol

      Volume: 88(13) Issue: 13 Pages: 7541-55

    • DOI

      10.1128/jvi.03170-13

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Alternative endocytosis pathway for productive entry of hepatitis C virus.2014

    • Author(s)
      Matsuda M, Suzuki R, Kataoka C, Watashi K, Aizaki H, Kato N, Matsuura Y, Suzuki T, Wakita T
    • Journal Title

      J Gen Virol.

      Volume: 95(Pt 12) Issue: 12 Pages: 2658-67

    • DOI

      10.1099/vir.0.068528-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 糖鎖基質酵素反応におけるマイクロ波効果の研究2016

    • Author(s)
      長島生、作田智美、杉山順一、佐々木正秀、清水弘樹
    • Organizer
      日本農芸化学会2016年大会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] マイクロ波を使用したペプチド固相合成法の必要条件の検討2015

    • Author(s)
      山田隆哉、長島生、佐々木正秀、清水弘樹
    • Organizer
      平成27年度日本農芸化学会北海道支部第2回講演会
    • Place of Presentation
      北海道大学学術交流会館(北海道)
    • Year and Date
      2015-11-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 化学反応におけるマイクロ波特性とマイクロ波効果の活用2015

    • Author(s)
      清水弘樹
    • Organizer
      化学工学会第47回秋季大会
    • Place of Presentation
      北海道大学工学部(北海道)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 反応容器による酵素反応の反応性差異2015

    • Author(s)
      清水弘樹、長島生
    • Organizer
      バイオ分子科学研究会
    • Place of Presentation
      慶応義塾大学日吉キャンパス(神奈川県)
    • Year and Date
      2015-08-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] beta-グルコース加水分解酵素反応における低周波マイクロ波効果の研究2015

    • Author(s)
      長島生、杉山順一、清水弘樹
    • Organizer
      日本農芸化学会2015年大会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 糖加水分解酵素反応におけるマイクロ波の効果と作用機構解明研究2014

    • Author(s)
      長島生、作田智美、杉山順一、清水弘樹
    • Organizer
      第33回日本糖質学会年会
    • Place of Presentation
      名古屋大学豊田講堂(愛知県)
    • Year and Date
      2014-08-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 耐熱グルコシダーゼHT1酵素反応におけるマイクロ波効果の研究2014

    • Author(s)
      長島生、作田智美、杉山順一、佐々木正秀、清水弘樹
    • Organizer
      日本農芸化学会2014年大会
    • Place of Presentation
      明治大学(神奈川)
    • Related Report
      2013 Annual Research Report
  • [Presentation] アスパラギン結合型糖鎖生合成に関与するドリコールリン酸結合7糖の合成研究2013

    • Author(s)
      宇津井隆志、宮沢進平、岩本将吾、松尾一郎
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪国際交流 センター(大阪)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 分岐型4糖オキサゾリン誘導体を用いたendo-Mの基質特異性解析2013

    • Author(s)
      石井希実、岩本将吾、熊田純一、松崎祐二、松尾一郎
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪国際交流 センター(大阪)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酵素反応へのマイクロ波利用研究

    • Author(s)
      清水弘樹、長島生、作田智美
    • Organizer
      第4回グライコバイオロジクス研究会
    • Place of Presentation
      札幌市民ホール(札幌)
    • Related Report
      2013 Annual Research Report
  • [Book] 糖鎖の新機能開発応用ハンドブック、第4編 第1章 4-4 マイクロ波合成2015

    • Author(s)
      長島生、清水弘樹
    • Publisher
      (株)エヌ・ティー・エス
    • Related Report
      2015 Annual Research Report
  • [Book] 最新 マイクロ波エネルギーと応用技術、第5章 5-3 生体高分子合成 (糖鎖、ペプチド、糖ペプチド)2014

    • Author(s)
      長島生、清水弘樹
    • Publisher
      ㈱産業技術サービスセンター
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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