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Whole Heart Engineering with Decellularization and in vivo Incubation

Research Project

Project/Area Number 25293276
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field General surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

GOJO Satoshi  京都府立医科大学, 医学(系)研究科(研究院), 教授 (90316745)

Co-Investigator(Kenkyū-buntansha) KAMI Daisuke  京都府立医科大学, 医学研究科, 講師 (80415588)
YAGI Hiroshi  慶応義塾大学, 医学部・外科学, 助教 (20327547)
MATOBA Satoaki  京都府立医科大学, 医学研究科, 教授 (10305576)
竹原 有史  旭川医科大学, 医学部, その他 (90374793)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords脱細胞 / 再細胞化 / 組織工学 / 界面活性剤 / 移植 / 心臓
Outline of Final Research Achievements

Whole organ tissue engineering for various organs has demonstrated promising results for end-stage organ failure. However, the sodium dodecyl sulfate (SDS)-based protocol for standard decellularization has drawbacks such as clot formation in vascularized transplantation and poor cell engraftment in recellularization procedures. Preservation of the surface milieu of extracellular matrices might be crucial . We identified a novel detergent, sodium lauryl ester sulfate (SLES), that it could overcome the drawbacks associated with SDS.
Porcine decellularized atrial appendage using SLES could function for a prosthesis material in the same location as the pretreated tissue. On the other hand, whole heart transplantation did not work due to massive thrombus in vessels at the level of the capillary. Present study achieved to improve the decellularization process well to enable the decellularized tissues to be grafted to in situ position.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Novel detergent for whole organ tissue engineering.2015

    • Author(s)
      Kawasaki T, Kirita Y, Kami D, Kitani T, Ozaki C, Itakura Y, Toyoda M, Gojo S.
    • Journal Title

      J. Biomed. Mater. Res. A.

      Volume: 103(10) Issue: 10 Pages: 3364-3373

    • DOI

      10.1002/jbm.a.35474

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cardiac Mesenchymal Progenitors from Postmortem Cardiac Tissues Retained Cellular Characterization2014

    • Author(s)
      Kami D, Kitani T, Nakata M, Gojo S
    • Journal Title

      Transplant. Proc.

      Volume: In press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Large-scale cell production of stem cells for clinical application using the automated cell processing machine.2013

    • Author(s)
      Kami D, Watakabe K, Yamazaki-Inoue M, Minami K, Kitani T, Itakura Y, Toyoda M, Sakurai T, Umezawa A, Gojo S.
    • Journal Title

      BMC Biotechnol

      Volume: 13 Issue: 1 Pages: 102-102

    • DOI

      10.1186/1472-6750-13-102

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Novel detergent for whole organ tissue engineering.2015

    • Author(s)
      Kawasaki T, Kirita Y, Kami D, Kitani T, Ozaki C, Itakura Y, Toyoda M, Gojo S.
    • Organizer
      2015 4th TERMIS World Congress
    • Place of Presentation
      Boston
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel detergent for organ decellularization can preserve cell surface structures.2014

    • Author(s)
      Kirita Y., Kawasaki T., Ishida R., Kitani T., Kami D., Adachi T., Gojo S.
    • Organizer
      International Society for Stem Cell Research 12th Annual Meeting.
    • Place of Presentation
      Vancouver
    • Year and Date
      2014-06-18 – 2014-06-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Cardiac mesenchymal progenitors from murine and simian post mortem cardiac tissue.2013

    • Author(s)
      Kami D, Nakata M, Kawasaki T, Kitani T, Gojo S.
    • Organizer
      The 13th Congress of the Asian Society of Transplantation. 2013
    • Place of Presentation
      Kyoto. Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Isolation of cardiac progenitors from murine and simian post mortem cardiac tissue.2013

    • Author(s)
      Kami D, Nakata M, Kitani T, Gojo S.
    • Organizer
      International Society for Stem Cell Research 11th Annual Meeting. 2013.
    • Place of Presentation
      Boston. USA.
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 脱細胞化処理液及び脱細胞化組織の製造方法2015

    • Inventor(s)
      五條理志
    • Industrial Property Rights Holder
      五條理志
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-172270
    • Filing Date
      2015-09-01
    • Related Report
      2015 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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