| Project/Area Number |
25293280
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
Jiro Fujimoto 兵庫医科大学, 医学部, 教授 (90199373)
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| Co-Investigator(Kenkyū-buntansha) |
KOSAKA Hisashi 兵庫医科大学, 医学部, 助教 (00532251)
IIMURO Yuji 山梨大学, 総合研究部, 医学研究員 (30252018)
OHASHI Koichiro 兵庫医科大学, 医学部, 病院助手 (50573987)
NAKANISHI Kenji 兵庫医科大学, 医学部, 学長 (60172350)
YOSHIMOTO Tomohiro 兵庫医科大学, 医学部, 教授 (60241171)
平野 公通 兵庫医科大学, 医学部, 准教授 (90340968)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 手術後癒着 / PAI-1 / IFN-g / t-PA / NKT cell / HGF / mesothelial cell / macrophage / 外科 / 癒着制御法 / 肝切除術 / 胆・膵手術 |
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| Outline of Final Research Achievements |
We investigated and obtained results of post-opertaive adhesion formation as follows. (1)We established a mouse model of adhesion formation after hepatectomy and found overexpression of IFN-g and PAI-1, downregulation of t-PA. Moreover the same mechanisms were noted in human hepatectomy. (2)We investigated fibrogenesis relating gene expression using cultured human mesothelial cells (Met-5A and MES-F). Overexpression of a-SMA, PDGF-R, and TGF-b-R were found and the mesothelial cells itself transformed into collagen producing cells. (3)We investigated which cells accumulated into the adhesion area after mouse hepatectomy. By immune-histopathology revealed that macrophage, T-cells, and neutrophils were identified in the tissue. Thus, mesothelial cells seems to play a pivotal role in fibrogenesis in adhesion formation after surgery.
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