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Roles of Runx2 and Runx3 in cartilage degeneration

Research Project

Project/Area Number 25293316
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionJichi Medical University (2014-2015)
The University of Tokyo (2013)

Principal Investigator

TAKESHITA Katsushi  自治医科大学, 医学部, 教授 (30262009)

Co-Investigator(Kenkyū-buntansha) SAITO Taku  東京大学, 医学部附属病院, 講師 (30456107)
TAKETOMI Shuji  東京大学, 医学部附属病院, 講師 (70570018)
上原 浩介  東京大学, 医学部附属病院, 特任臨床医 (20599063)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords整形外科学 / 変形性関節症 / 軟骨細胞 / 骨軟骨代謝学
Outline of Final Research Achievements

We analyzed roles of transcription factor Runx2 and Runx3 in articular chondrocytes. First, we generated Runx2-flox mice, and confirmed that the gene deletion was successful. We then crossed Runx2-flox mice with cartilage-specific Cre mice, and created osteoarthritis model. Deletion of these factors modified cartilage degeneration. We analyzed the molecular mechanisms by in vitro experiments.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (27 results)

All 2016 2015 2014 2013 Other

All Journal Article (17 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 17 results,  Open Access: 7 results,  Acknowledgement Compliant: 5 results) Presentation (9 results) (of which Invited: 5 results) Remarks (1 results)

  • [Journal Article] Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment.2016

    • Author(s)
      1.Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, Kataoka K, Saito T, Chung UI, Ohba S.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 18743-18743

    • DOI

      10.1038/srep18743

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparison of mouse and human ankles and establishment of mouse ankle osteoarthritis models by surgically-induced instability2016

    • Author(s)
      Chang SH, Yasui T, Taketomi S, Matsumoto T, Kim-Kaneyama JR, Omiya T, Hosaka Y, Inui H, Omata Y, Yamagami R, Mori D, Yano F, Chung U, Tanaka S, Saito T.
    • Journal Title

      Osteoarthritis Cartilage

      Volume: 24 Issue: 4 Pages: 688

    • DOI

      10.1016/j.joca.2015.11.008

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Predictive Factors for Subjective Improvement in Lumbar Spinal Stenosis Patients with Nonsurgical Treatment: A 3-Year Prospective Cohort Study.2016

    • Author(s)
      Matsudaira K, Hara N, Oka H, Kunogi J, Yamazaki T, Takeshita K, Atsushi S, Tanaka S.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 2 Pages: e0148584-e0148584

    • DOI

      10.1371/journal.pone.0148584

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] <b>Hyaline cartilage formation and tumorigenesis of implanted tissues derived from human induced pluripotent stem </b><b>cells </b>2015

    • Author(s)
      Saito T, Yano F, Mori D, Kawata M, Hoshi K, Takato T, Masaki H, Otsu M, Eto K, Nakauchi H, Chung UI, Tanaka S
    • Journal Title

      Biomedical Research

      Volume: 36 Issue: 3 Pages: 179-186

    • DOI

      10.2220/biomedres.36.179

    • NAID

      130005083858

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Matsubayashi Y, Takeshita K, Sumitani M, Oshima Y, Tonosu J, Kato S, Ohya J, Oichi T, Okamoto N, Tanaka S. PLoS One. 2012015

    • Author(s)
      Psychometric Validation of the Japanese Version of the Neuropathic Pain Symptom Inventory.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 11 Pages: e0143350-e0143350

    • DOI

      10.1371/journal.pone.0143350

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcription factor Hes1 modulates osteoarthritis development in cooperation with calcium/calmodulin-dependent protein kinase 2.2015

    • Author(s)
      Sugita S, Taketomi S, Saito T, et al.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 112 Issue: 10 Pages: 3080-5

    • DOI

      10.1073/pnas.1419699112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] <b>Regulation of mouse chondrocyte differentiation by CCAAT/enhancer-binding pr</b><b>oteins </b>2015

    • Author(s)
      Okuma T, Hirata M, Yano F, Mori D, Kawaguchi H, Chung UI, Tanaka S, Saito T
    • Journal Title

      Biomedical Research

      Volume: 36 Issue: 1 Pages: 21-29

    • DOI

      10.2220/biomedres.36.21

    • NAID

      130004903876

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of the Japanese Orthopaedic Association (JOA) Score and Modified JOA (mJOA) Score for the Assessment of Cervical Myelopathy: A Multicenter Observational Study.2015

    • Author(s)
      Kato S, Takeshita K, et al.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 4 Pages: e0123022-e0123022

    • DOI

      10.1371/journal.pone.0123022

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Elevated Levels of Phosphorylated Neurofilament Heavy Subunit in the Cerebrospinal Fluid of Patients with Lumbar Spinal Stenosis: Preliminary Findings.2015

    • Author(s)
      Ohya J, Takeshita K, et al.
    • Journal Title

      Spine J

      Volume: 未定 Issue: 7 Pages: 1587-1592

    • DOI

      10.1016/j.spinee.2015.03.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Patient satisfaction with double-door laminoplasty for cervical compression myelopathy.2015

    • Author(s)
      Ohya J, Takeshita K, et al.
    • Journal Title

      J Orthop Sci

      Volume: 20 Issue: 1 Pages: 64-70

    • DOI

      10.1007/s00776-014-0666-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of SCAN domain zinc-finger gene ZNF449 as a novel factor of chondrogenesis2014

    • Author(s)
      Okada K, Saito T, et al.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 12 Pages: e115169-e115169

    • DOI

      10.1371/journal.pone.0115169

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Disease-modifying effects of TD-198946 on progressed osteoarthritis in a mouse model.2014

    • Author(s)
      Yano F, Saito T, et al.
    • Journal Title

      Ann Rheum Dis.

      Volume: 73 Issue: 11 Pages: 2062-4

    • DOI

      10.1136/annrheumdis-2014-205672

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphological characteristics of cervical spine in patients with athetoid cerebral palsy and the accuracy of pedicle screw placement.2014

    • Author(s)
      Kato S, Takeshita K, et al.
    • Journal Title

      Spine

      Volume: 39 Issue: 8 Pages: E508-E513

    • DOI

      10.1097/brs.0000000000000234

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of fibroblast growth factor-18 as a molecule to protect adult articular cartilage by gene expression profiling.2014

    • Author(s)
      Mori Y, Saito T, Chang SH, Kobayashi H, Ladel CH, Guehring H, Chung UI, Kawaguchi H.
    • Journal Title

      J Biol Chem

      Volume: 289 Issue: 14 Pages: 10192-200

    • DOI

      10.1074/jbc.m113.524090

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Positive feedback between NF-κB and TNF-α promotes leukemia-initiating cell capacity.2014

    • Author(s)
      Kagoya Y, Yoshimi A, Kataoka K, Nakagawa M, Kumano K, Arai S, Kobayashi H, Saito T, Iwakura Y, Kurokawa M.
    • Journal Title

      J Clin Invest.

      Volume: 124 Issue: 2 Pages: 528-542

    • DOI

      10.1172/jci68101

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of Col2a1-EGFP iPS cells for monitoring chondrogenic differentiation2013

    • Author(s)
      Saito T, Yano F, Mori D, Ohba S, Hojo H, Otsu M, Eto K, Nakauchi H, Tanaka S, Chung UI, Kawaguchi H
    • Journal Title

      PLoS One

      Volume: 16;8(9) Issue: 9 Pages: e74137-e74137

    • DOI

      10.1371/journal.pone.0074137

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional induction of ADAMTS5 protein by nuclear factor-κB (NF-κB) family member RelA/p65 in chondrocytes during osteoarthritis development.2013

    • Author(s)
      Kobayashi H, Hirata M, Saito T, Itoh S, Chung UI, Kawaguchi H.
    • Journal Title

      J Biol Chem.

      Volume: 288 Issue: 40 Pages: 28620-9

    • DOI

      10.1074/jbc.m113.452169

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 転写因子を起点とした変形性関節症のメカニズム2016

    • Author(s)
      齋藤琢他
    • Organizer
      第29回日本軟骨代謝学会
    • Place of Presentation
      広島
    • Year and Date
      2016-02-19
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Notch/Rbpj/Hes1経路による変形性関節症の制御機構2015

    • Author(s)
      齋藤琢他
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 変形性関節症の分子メカニズム2015

    • Author(s)
      齋藤琢他
    • Organizer
      第15回日本抗加齢医学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Establishment of Surgical Destabilization Model of Mouse Ankle Osteoarthritis.2015

    • Author(s)
      Chang SH, Saito T, et al.
    • Organizer
      Annual meeting of the Orthopaedic Research Society.
    • Place of Presentation
      Las vegas, USA
    • Year and Date
      2015-03-28 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of Grem1 as a catabolic factor induced by mechanical stress loading in articular chondrocyte.2014

    • Author(s)
      Chang SH, Saito T, et al.
    • Organizer
      Cold Spring Harbor Asia conferences.
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2014-11-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 変形性関節症への応用を目指した軟骨再生研究2014

    • Author(s)
      齋藤琢
    • Organizer
      第29回日本整形外科学会基礎学術集会
    • Place of Presentation
      鹿児島
    • Year and Date
      2014-10-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] HIF-1α IS ESSENTIAL FOR ARTICULAR CARTILAGE HOMEOSTASIS THROUGH INDUCTION OF ANABOLIC FACTORS AND SUPPRESSION OF CATABOLIC FACTORS.2014

    • Author(s)
      Okada K, Saito T, et al.
    • Organizer
      Annual meeting of the American Society for bone and mineral research.
    • Place of Presentation
      Houston
    • Year and Date
      2014-09-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of Grem1 as a catabolic factor induced by mechanical stress loading in articular chondrocyte.2014

    • Author(s)
      Chang SH, Saito T, et al.
    • Organizer
      Annual meeting of the American Society for bone and mineral research.
    • Place of Presentation
      Houston
    • Year and Date
      2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] HIFによる関節軟骨の変性メカニズム2013

    • Author(s)
      3) 齋藤琢
    • Organizer
      日本整形外科学会基礎学術集会
    • Place of Presentation
      千葉
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks] 東京大学医学部付属病院 整形外科

    • URL

      http://www.u-tokyo-ortho.jp/

    • Related Report
      2014 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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