Analysis of murine fetal cutaneous regeneration using laser micro dissection system

• Project/Area Number: 25293363
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: Kishi Kazuo 慶應義塾大学, 医学部, 教授 (40224919)
• Co-Investigator(Kenkyū-buntansha): KUBOTA YOSHIAKI 慶應義塾大学, 医学部, 教授 (50348687) ; HAYASHI RUKA 慶應義塾大学, 医学部, 助教 (50445392) ; ARAMAKI NORIKO 慶應義塾大学, 医学部, 講師 (80365311)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 皮膚 / 再生 / 胎仔 / 創傷 / 再生医療 / レーザーマイクロダイセクション / マイクロアレイ

Outline of Final Research Achievements

Fetuses, to some developmental stages, have an ability to regenerate skin.
We discovered that until embryonic day (E) 13, skin regenerate completely, but after E14, visible mark is left. Based on these data, we compared E13 and E15 wounds using laser micro dissection system and microarray.