Development of Chemically Defined Culture Substrate for Cell Proliferation and Differentiation

• Project/Area Number: 25293403
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Hirata Isao 広島大学, 医歯薬保健学研究院(歯), 助教 (40346507)
• Co-Investigator(Kenkyū-buntansha): KANAWA Masami 広島大学, 自然科学研究支援開発センター, 助教 (00284208) ; KATO Koichi 広島大学, 大学院医歯薬保健学研究院(歯), 教授 (50283875) ; KATO Yukio 広島大学, 大学院医歯薬保健学研究院(歯), 研究員 (10112062)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
• Keywords: 再生医学 / 表面・界面物性 / 細胞・組織 / ナノ薄膜 / 表面･界面物性 / 細胞･組織 / 間葉系幹細胞 / 幹葉系幹細胞

Outline of Final Research Achievements

A study of cell culture substrates requires the technology of the surface physical chemistry in addition to biology, so these progresses are slow. However this research is very important because the scaffold on which cells to live. This study was aimed precise control of proliferation and differentiation of cells, we developed a chemically defined culture substrate whose surface precisely controlled in nano-order. In particular cause of improvement of proliferation of mesenchymal stem cells on well-defined substrate, it has been suggested to be due to the maintenance of stemness.

Research Products

• [Presentation] Effect of Mesenchymal Stem Cells from Surface Functional Groups on Mixed Self Assembled Monolayer (2016)
  • Author(s): Hirata I, Veronica SR, Tania S, Yamauchi Y, Kanawa M, Kato Y, Kato K
  • Organizer: International Dental Materials Congress 2016
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2016-11-04
  • Int'l Joint Research
• [Presentation] 間葉系幹細胞無血清培地に適した培養基材の開発 ｰ自己組織化単分子膜による検討ｰ (2015)
  • Author(s): 平田伊佐雄，山内優佳，Tania Saskianti，Veronica Sainik Ronald，金輪真佐美，加藤幸夫，加藤功一
  • Organizer: 第37回日本バイオマテリアル学会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2015-11-09
• [Presentation] 間葉系幹細胞用無血清培地に適した培養基材の開発 (2015)
  • Author(s): 平田伊佐雄, 山内優佳, 金輪真佐美, 加藤幸夫, 加藤功一
  • Organizer: 日本組織培養学会第88回大会
  • Place of Presentation: 広島大学 霞キャンパス内 広仁会館
  • Year and Date: 2015-05-26
• [Presentation] 間葉系幹細胞の無血清培養のための培養基材の開発 (2015)
  • Author(s): 山内優佳，平田伊佐雄，金輪真佐美，加藤幸夫，加藤功一
  • Organizer: 第65 回日本歯科理工学会学術講演会プログラム
  • Place of Presentation: 仙台市情報・産業プラザ
  • Year and Date: 2015-04-11
• [Presentation] 間葉系幹細胞に特化した培養基板の開発 -自己組織化単分子膜と無血清培地による検討- (2014)
  • Author(s): 平田伊佐雄，Veronica Sainik Ronald，金輪真佐美，加藤幸夫，加藤功一
  • Organizer: 第64回日本歯科理工学会
  • Place of Presentation: 広島市
  • Year and Date: 2014-10-04 – 2014-10-05
• [Presentation] 混合自己組織化単分子膜上の表面官能基による細胞の影響 (2014)
  • Author(s): 平田伊佐雄、加藤功一
  • Organizer: 第144回ポバール会
  • Place of Presentation: 京都市
  • Year and Date: 2014-07-05
• [Presentation] Surface Design for Optimizing Mesenchymal Stem Cell Culture in a Serum-free Medium (2014)
  • Author(s): Yuka Yamauchi, Isao Hirata, Masami Kanawa, Yukio Kato, Koichi Kato
  • Organizer: The 2nd Japan-China Symposium on Nanomedicine
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2014-05-16 – 2014-05-17