Chemopreventive intervention study and miRNA monitoring of oral cancer in Sri Lanka

• Project/Area Number: 25305036
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Surgical dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: Chiba Itsuo 北海道医療大学, 歯学部, 教授 (50250460)
• Co-Investigator(Kenkyū-buntansha): 松岡 紘史 北海道医療大学, 歯学部, 講師 (50598092) ; 安彦 善裕 北海道医療大学, 歯学部, 教授 (90260819)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: betel quid / 口腔がん / スリ・ランカ / 介入試験 / cur cumin / MMP-9 / arecoline / 化学予防 / 口腔前がん病変 / 介入研究 / curcumin / Oral cancer / betel quid chewing / Sri Lanka / Biomarker / 南アジア / アレコリン / miRNA / バイオマーカー / MMP9 / 前がん病変

Outline of Final Research Achievements

We have established cell culture model for analyzing epigenetic changes caused by arecoline, one of ingredients of betel quid. Using DNA microarray analysis and bioinformatics analysis, we clarified that mRNA level of MMP-9 was elevated and curcumin suppressed the mRNA level.
Chemopreventive intervention study has started in the tea estates of Sri Lanka. Because of the short period of observation, we have not got the results.

Research Products

• [Journal Article] Upregulated expression of MMP-9 in gingival epithelial cells induced by prolonged stimulation with arecoline (2017)
  • Author(s): Osamu Uehara, Kousuke Takimoto, Tetsuro Morikawa, Fumiya Harada, Bhoj Raj Adhikari, Ryoko Itatsu, Tomohisa Nakamura, Rie Takai, Koki Yoshida, Hiroki Nagayasu, Ichiro Saito,, Malsantha, Muthumala Itsuo Chiba, Yoshihiro Abiko
  • Journal Title: Oncology Letters Volume: 印刷中
• [Journal Article] アジアにおけるベテル噛みの習慣と口腔がんの発生及びその予防 (2014)
  • Author(s): 千葉逸朗、杉浦千尋、小野寺麻記子
  • Journal Title: 北海道公衆衛生学雑誌 Volume: 28 Pages: 27-36
  • Peer Reviewed
• [Presentation] Oral Cancer Prevention in Sri Lanka (2015)
  • Author(s): 千葉逸朗
  • Organizer: 2nd World Congress of Oral & Dental Medicine
  • Place of Presentation: 大連（中国）
  • Year and Date: 2015-11-02
  • Int'l Joint Research
• [Presentation] アレコリン長期刺激による歯肉上皮細胞のMMP-9遺伝子への影響 (2015)
  • Author(s): 植原 治，瀧本紘佑，宮本一央，原田文也，小野寺麻記子，杉浦千尋，永易裕樹，安彦善裕，千葉逸朗
  • Organizer: 日本口腔外科学会総会・学術大会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-16 – 2015-10-18
• [Presentation] アレコリン長期刺激による歯肉上皮細胞に対する網羅的遺伝子解析 (2015)
  • Author(s): 植原治、松岡紘史、藤田真理、川上智史、千葉逸朗
  • Organizer: 第６４回に本口腔衛生学会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2015-05-29
• [Presentation] アレコリン長期刺激による歯肉上皮細胞に対する網羅的遺伝子解析 (2015)
  • Author(s): 植原治、松岡紘史、藤田真理、川上智史、千葉逸朗
  • Organizer: 日本口腔衛生学会総会・学術大会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2015-05-27 – 2015-05-29