Development of construction and application of stadndard brain protocol and software environment for various animal spiceis

• Project/Area Number: 25330342
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Life / Health / Medical informatics
• Research Institution: University of Hyogo
• Principal Investigator: Ikeno Hidetoshi 兵庫県立大学, 環境人間学部, 教授 (80176114)
• Co-Investigator(Kenkyū-buntansha): Ai Hiroyuki 福岡大学, 理学部, 助教 (20330897) ; Kanzaki Ryohei 東京大学, 先端科学技術研究センター, 教授 (40221907) ; Nishikawa Ikuko 立命館大学, 情報理工学部, 教授 (90212117)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ニューロン / セグメンテーション / 標準脳 / レジストレーション / 画像処理 / 神経細胞形態 / 主成分分析 / ミツバチ / カイコガ / 神経細胞形態モデル / 神経回路シミュレーション / ニューロインフォマティクス / 昆虫 / データベース

Outline of Final Research Achievements

In this study, we developed a protocol and software tools to construct and use the standard brain for integrating and sharing morphological information about brain and neurons. Our protocol was applied to construction of moth’s standard brain. It was shown that the method could be applied to other species, such as honeybee. Our segmentation software was increased in performance and operability, then provided to use on various operating systems. Our neuron segmentation and description methods were applied to other academic field, tree root science, which target at similar morphological characteristics.

Research Products

• [Journal Article] Walking patterns induced by learned odors in the honeybee, Apis mellifera L. (2016)
  • Author(s): Yamashita, T., Haupt, S. S., Ikeno, H., Ai, H.
  • Journal Title: Journal of Experimental Biology Volume: 219 Pages: 12-16
  • DOI: 10.1242/jeb.123356
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 減災の観点から樹木根系を非破壊的に推定する地中レーダ法の現状と課題 (2015)
  • Author(s): 平野恭弘, 山瀬敬太郎, 谷川東子, 檀浦正子, 檀浦正子, 大橋瑞江, 藤堂千景, 池野英利
  • Journal Title: 日本緑化工学会誌 Volume: 41 Pages: 319-325
  • Peer Reviewed
• [Journal Article] 間伐がスギの最大引き倒し抵抗モーメントにもたらす影響 (2015)
  • Author(s): 藤堂千景, 山瀬敬太郎, 谷川東子, 大橋瑞江, 池野英利, 檀浦正子, 檀浦正子, 平野恭弘
  • Journal Title: 日本緑化工学会誌 Volume: 41 Pages: 309-314
  • Peer Reviewed
• [Journal Article] 異なる土壌環境下における根系構造と引き抜き抵抗力との関係 (2015)
  • Author(s): 山瀬敬太郎, 谷川東子, 池野英利, 藤堂千景, 大橋瑞江, 檀浦正子, 檀浦正子, 平野恭弘
  • Journal Title: 日本緑化工学会誌 Volume: 41 Pages: 301-307
  • Peer Reviewed
• [Journal Article] NeuronDepot – Keeping your colleagues in sync by combining modern cloud storage services, the local system, and simple web applications. (2014)
  • Author(s): Rautenberg, P. L., Kumaraswamy, A., Tejero-Cantero, A., Doblander. C., Norouzian, M., Kai, K., Jacobsen, H-A, Ai, H., Wachtler, T., Ikeno, H.
  • Journal Title: Frontiers in Neuroinformatics Volume: April Pages: 1-16
  • DOI: 10.3389/fninf.2014.00055
  • Peer Reviewed / Open Access
• [Journal Article] Antennal lobe organization and pheromone usage in bombycid moths (2014)
  • Author(s): Shigehiro Namiki, Takaaki Daimon, Chika Iwatsuki, Toru Shimada, Ryohei Kanzaki
  • Journal Title: Biology Letters Volume: 10 Issue: 4 Pages: 20140096-20140096
  • DOI: 10.1098/rsbl.2014.0096
  • Peer Reviewed / Open Access
• [Journal Article] Information flow through neural circuits for pheromone orientation in the moth (2014)
  • Author(s): Namiki S, Iwabuchi S, Pansopha Kono P, Kanzaki R
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 5919-5919
  • DOI: 10.1038/ncomms6919
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 鱗翅類の翅周縁部に分布する振動受容感覚器 (2014)
  • Author(s): 吉田明宏、藍浩之
  • Journal Title: 昆虫と自然 Volume: 49 Pages: 11-16
• [Journal Article] Effect of olfactory stumulus on the flight course of a honeybee, Apis mellifera, in a wind tunnel. (2014)
  • Author(s): Ikeno, H., Akamatsu, T., Hasegawa, Y., Ai, H.
  • Journal Title: Insects Volume: 5 Issue: 1 Pages: 92-104
  • DOI: 10.3390/insects5010092
  • Peer Reviewed / Open Access
• [Journal Article] Development of a new method to track multiple honey bees with complex behaviors on a flat laboratory arena (2014)
  • Author(s): Kimura, T., Ohashi. M., Crailsheim, K., Schmickl, T., Okada, R., Radspieler, G., Ikeno, H.
  • Journal Title: PLoS ONE Volume: 9 Issue: 1 Pages: e84656-e84656
  • DOI: 10.1371/journal.pone.0084656
  • Peer Reviewed / Open Access
• [Journal Article] Error in the honeybee waggle dance improves foraging flexibility. (2014)
  • Author(s): Ryuichi Okada, Hidetoshi Ikeno, Toshifumi Kimura, Mizue Ohashi, Hitoshi Aonuma & Etsuro Ito
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 4175-4175
  • DOI: 10.1038/srep04175
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Root orientation can affect detection accuracy of ground-penetrating radar. (2013)
  • Author(s): Tanikawa, T., Hirano, Y., Dannoura, M., Yamase, K., Aono, K., Ishii, M., Igarashi, T., Ikeno, H., Kanazawa, Y.
  • Journal Title: Plant Soil Volume: 373 Issue: 1-2 Pages: 317-327
  • DOI: 10.1007/s11104-013-1798-6
  • Peer Reviewed
• [Journal Article] Morphological analysis of the primary center receiving spatial information transferred by the waggle dance of honeybees. (2013)
  • Author(s): Ai, H., Hagio, H.
  • Journal Title: J. Comp. Neurol. Volume: 521 Issue: 11 Pages: 2570-2584
  • DOI: 10.1002/cne.23299
  • Peer Reviewed
• [Journal Article] Sensors and sensory processing for airborne vibrations in silk moths and honeybees. (2013)
  • Author(s): Ai, H.
  • Journal Title: Sensors Volume: 13 Issue: 7 Pages: 9344-9363
  • DOI: 10.3390/s130709344
  • Peer Reviewed
• [Presentation] Honeybee interneurons responsive to the pulsed vibration produced by waggle dance (2016)
  • Author(s): Ai, H., Kai, K., Watanabe, H., Itoh, T., Kumaraswamy, A, Rautenberg, P. Wachtler, T, Ikeno, H.
  • Organizer: International congress of neuroethology 2016
  • Place of Presentation: Uruguay
  • Year and Date: 2016-03-30
  • Int'l Joint Research / Invited
• [Presentation] ミツバチの採餌行動におけるエネルギー収支 (2016)
  • Author(s): 澤井穂高，岡田龍一,吉田澪,大橋瑞江，木村敏文，池野英利
  • Organizer: 日本生態学会第63回全国大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-20
• [Presentation] Seasonal changes of energy gain and consumption in a honeybee colony (2016)
  • Author(s): YOSHIDA,M.,OHASHI,M.,KIMURA,T.,OKADA,R.,IKENO,H.
  • Organizer: 日本生態学会第63回全国大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-20
• [Presentation] Phototaxis behavior of ambrosia beetle Platypus quercivorus before and after flight (2016)
  • Author(s): Pham Duy Long, Michimasa Yamasaki, Yasuto Ito, Ryuichi Okada, Hidetoshi Ikeno
  • Organizer: 日本生態学会第63回全国大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-20
• [Presentation] レーダを用いた樹木根系の非かく乱調査 -海岸ク ロマツ個体の水平根の広がり- (2016)
  • Author(s): 大橋瑞江,池野英利,柿添哲也,山瀬敬太郎, 谷川東子,檀浦正子,藤堂千景, 平野恭弘
  • Organizer: 日本生態学会第63回全国大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-20
• [Presentation] Comprehensive analyses of interneurons arborizing in the primary vibration center in honeybee brain. (2015)
  • Author(s): Ai, H., Kai, K., Watanabe, H., Kumaraswamy, A, Rautenberg, P. Wachtler, T, Ikeno, H. Ai, H
  • Organizer: CompBiol 2015
  • Place of Presentation: 広島
  • Year and Date: 2015-12-11
• [Presentation] A method for construction and application of standard brain model based on the confocal images (2015)
  • Author(s): H. Ikeno, D. Miyamoto, T. Kazawa, R. Kanzaki
  • Organizer: Advances in Neuroinformatics2015 tutorial session
  • Place of Presentation: 東京
  • Year and Date: 2015-11-26
  • Int'l Joint Research / Invited
• [Presentation] Development of protocol and software for registration in situ hybridization image into the standard brain (2015)
  • Author(s): H. Ikeno, D. Miyamoto, Y. Okamura-Oho, H. Nishibe, A. Sato, T. Furuichi, R. Kanzaki
  • Organizer: Advances in Neuroinformatics 2015
  • Place of Presentation: 東京
  • Year and Date: 2015-11-26
  • Int'l Joint Research
• [Presentation] 学習した匂いと尻振りダンス振動によって誘発されるミツバチの歩行の解析 (2015)
  • Author(s): 山下俊也、池野英利、長谷川雄二、伊東綱男、藍 浩之
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-17
• [Presentation] Development of an on-line simulation platform for neuroscience research (2015)
  • Author(s): A. Ishihara, H. Ikeno, H. Wagatsuma, K. Inagaki, S. Usui, S. Satoh, T. Yamazaki, T. Kannon, Y. Yamaguchi, Y. Okumura, Y. Kamiyama, Y. Asai, Y. Hirata
  • Organizer: INCF Congress Neuroinformatics 2015
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2015-08-20
  • Int'l Joint Research
• [Presentation] ミツバチにおける学習した匂いによって誘発される歩行の経時変化と音刺激による影響 (2015)
  • Author(s): 山下俊也、Haupt, S.S.、池野英利、伊東綱男、藍 浩之
  • Organizer: 日本動物学会九州支部第68回大会（三学会合同福岡大会）
  • Place of Presentation: 福岡
  • Year and Date: 2015-05-23
• [Presentation] The parallel systems in the primary auditory center of the honeybee. (2015)
  • Author(s): Hiroyuki Ai
  • Organizer: 11th Goettingen Meeting of German Neuroscience Society
  • Place of Presentation: Goettingen, Germany
  • Year and Date: 2015-03-18 – 2015-03-21
  • Invited
• [Presentation] Segmentation of neuronal structure from confocal image and its applications (2015)
  • Author(s): Hidetoshi Ikeno
  • Organizer: Meeting Honeybee Standard Brain
  • Place of Presentation: Freie Universitat Berlin, Berlin, Germany
  • Year and Date: 2015-03-16 – 2015-03-17
  • Invited
• [Presentation] Topological organization of vibration-sensitive neurons of honeybee (2015)
  • Author(s): Hiroyuki Ai
  • Organizer: Meeting Honeybee Standard Brain
  • Place of Presentation: Universitat Berlin, Berlin, Germany
  • Year and Date: 2015-03-16 – 2015-03-17
  • Invited
• [Presentation] 昆虫嗅覚系全脳シミュレーション (2015)
  • Author(s): 宮本大輔，加沢知毅，後藤昂彦，朴希原，神崎亮平
  • Organizer: バイオスーパーコンピューティング研究会ウインタースクール
  • Place of Presentation: 休暇村伊良湖（愛知、田原）
  • Year and Date: 2015-01-30 – 2015-01-31
• [Presentation] Neural Basis of Odor-Source Localization in the Silkmoth: from genes, neural networks, and behavior to robots. (2015)
  • Author(s): Kanzaki R
  • Organizer: Annual Talks 2015 - Biology Across Scales
  • Place of Presentation: Bangalore, India
  • Year and Date: 2015-01-05 – 2015-01-08
  • Invited
• [Presentation] Simulation Platform: Application Server for Testing and Sharing Mathematical Model and Experimental Data (2014)
  • Author(s): Hidetoshi IKENO, Yoshimi KAMIYAMA, Akito ISHIHARA, Yutaka HIRATA, Shunji SATOH, Tadashi YAMAZAKI, Hiroaki WAGATSUMA, Yoshihiro OKUMURA, Keiichiro INAGAKI, Takayuki KANNON, Yoshiyuki ASAI, Yoko YAMAGUCHI, Shiro USUI
  • Organizer: INCF Japan Node International Workshop: Advances in Neuroinformatics 2014
  • Place of Presentation: 理化学研究所（埼玉、和光）
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] The Invertebrate Brain Platform (IVB-PF) - Comparative Expositions and Data Collection about Invertebrate Brain Platform (2014)
  • Author(s): Ryohei KANZAKI, Tomoki KAZAWA, Akira TAKASHIMA, Sakiko SHIGA, Hidetoshi IKENO, Yoko YAMAGUCHI
  • Organizer: INCF Japan Node International Workshop: Advances in Neuroinformatics 2014
  • Place of Presentation: 理化学研究所（埼玉、和光）
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] Acceleration of NEURON simulator for morphological detailed multi-compartment Hodgkin-Huxley type simulation toward real-time speed. (2014)
  • Author(s): Daisuke Miyamoto, Tomoki Kazawa, Akihiko Goto and Ryohei Kanzaki
  • Organizer: AINI2014
  • Place of Presentation: 理化学研究所（埼玉、和光）
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] Constructing multi-compartment parallelized simulation from olfactory input to premotor command generation of silkmoth brain. (2014)
  • Author(s): Tomoki Kazawa, Daisuke Miyamoto, Akihito Goto, Heewon Park, Hidetoshi Ikeno, Ikuko Nishikawa and Ryohei Kanzaki
  • Organizer: AINI2014
  • Place of Presentation: 理化学研究所（埼玉、和光）
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] ミツバチ脳内の触角機械感覚運動中枢からキノコ体に投射する介在ニュー ロン (2014)
  • Author(s): 甲斐 加樹来,池野 英利, 藍 浩之
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 東北大学（宮城、仙台）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Constructing a massively parallelized morphological detailed neural circuit simulation of silkmoth brain with neuron database (2014)
  • Author(s): Miyamoto D, Kazawa T, Goto A , Ikeno H, Kanzaki R
  • Organizer: Joint meeting of the 11th International Neuroethology Conference and the 36th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌）
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] Reproducible segmentation method of neural morphology from LSM images (2014)
  • Author(s): Hidetoshi Ikeno, Kazuki Kai, Sho Iizuka, Ajayrama Kumaraswamy, Philipp Rautenberg, Thomas Wachtler, Hiroyuki Ai
  • Organizer: Joint meeting of the 11th International Neuroethology Conference and the 36th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌）
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] Neural basis of vibratory signal processing of the honeybee Apis mellifera. (2014)
  • Author(s): Kazuki Kai, Ajayrama Kumaraswamy, Philipp Rautenberg, Thomas Wachtler, Hidetoshi Ikeno, Hiroyuki Ai
  • Organizer: Joint meeting of the 11th International Neuroethology Conference and the 36th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌）
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] How does the waggle dance communication mature after the adult emergence? (2014)
  • Author(s): Hiroyuki Ai and Noriko Kishi
  • Organizer: The 11th International Congress of Neuroethology (2014 ICN/JSCPB)
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌）
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] Sensory responses to the oriental orchid odors in the Japanese and European honeybees. (2014)
  • Author(s): Ayaka Harada, Hiroyuki Ai, Michio Sugahara, Ryuichi Okada, Midori Sakura
  • Organizer: The 11th International Congress of Neuroethology (2014 ICN/JSCPB)
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌）
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] Analysis and synthesis of odor-source localization in insects: From genes, neural networks, and behavior to robots. (2014)
  • Author(s): Kanzaki R
  • Organizer: 11th International Congress of Neuroethology / 36th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌）
  • Year and Date: 2014-07-28 – 2014-08-01
  • Invited
• [Presentation] Toward collaboration in NIJC platforms: Standard brain database linked with application server, (2013)
  • Author(s): Hidetoshi Ikeno, Tomoki Kazawa, Ryohei Kanzaki, Yoshihiro Okumura, Yoko Yamaguchi and Shiro Usui
  • Organizer: INCF Congress Neuroinformatics 201
  • Place of Presentation: Karolinska Institutet(Stockholm, Sweden)
• [Presentation] Response properties of auditory interneurons in the honeybee brain (2013)
  • Author(s): Kazuki Kai, Hidetoshi Ikeno, Stephan S. Haupt, Philipp L. Rautenberg, Thomas Wachtler, Hiroyuki Ai
  • Organizer: Bernstein Conference
  • Place of Presentation: Neue Aula (Tuebingen, Germany)
• [Presentation] Constructing a multi-compartment parallelized simulation of a premotor area of the silkmoth brain (2013)
  • Author(s): Tomoki Kazawa, Yusuke Mori, Daisuke Miyamoto, Shigehiro Namiki, Hidetoshi Ikeno, Stephan Shuichi Haupt, Ikuko Nishikawa, Ryohei Kanzaki
  • Organizer: 日本比較生理学会第35回大会
  • Place of Presentation: イーグレ姫路（兵庫県姫路市）
• [Presentation] Development of a tracking program, K-Track, for analyzing honeybee’s behaviors (2013)
  • Author(s): Toshifumi Kimura, Mizue Ohashi, Karl Crailsheim, Thomas Schmickl, Ryuichi Okada, Gerald Radspieler, Hidetoshi Ikeno
  • Organizer: 日本比較生理学会第35回大会
  • Place of Presentation: イーグレ姫路（兵庫県姫路市）
• [Presentation] Physiology and morphology of antenna-vibration sensitive neurons in the dorsal lobe of the honey bee, Apis mellifera (2013)
  • Author(s): Kazuki Kai, Hidetoshi Ikeno, Hiroyuki Ai
  • Organizer: 日本比較生理学会第35回大会
  • Place of Presentation: イーグレ姫路（兵庫県姫路市）
• [Presentation] 学習した匂い刺激により生じる歩行様式の性質とその季節変動 (2013)
  • Author(s): 藍 浩之、宮本奈央、山本千尋、石橋祥太、山下俊也
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山大学（岡山県岡山市）
• [Presentation] 昆虫全脳シミューレーションに向けたスーパーコンピュータとのリアルタイム通信の検討 (2013)
  • Author(s): 森 友亮・加沢知毅・宮本大輔・神崎亮平
  • Organizer: 電子情報通信学会ニューロコンピューティング（NC）研究会
  • Place of Presentation: 新潟大学（新潟県新潟市）
• [Presentation] ミツバチの尻振りダンスの発現と脳内構造の定量的解析
  • Author(s): 藍 浩之
  • Organizer: 第3回ミツバチシンポジウム
  • Place of Presentation: 兵庫県立大学（兵庫県姫路市）
• [Book] サイボーグ昆虫、フェロモンを追う (2014)
  • Author(s): 神崎亮平
  • Total Pages: 108
  • Publisher: 岩波書店