Development and application of detection methods for genes like the ones for ammonia- or methane- oxidation
Project/Area Number |
25340016
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Environmental dynamic analysis
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Research Institution | Shibaura Institute of Technology |
Principal Investigator |
Fuse Hiroyuki 芝浦工業大学, システム工学部, 教授 (30357925)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | メタンモノオキシゲナーゼ遺伝子 / 短鎖炭化水素資化性菌 / メタン酸化細菌 / メタンモノオキシゲナーゼ / 短鎖アルカン資化性菌 / アンモニア酸化細菌 / メタン資化性菌 / メタン酸化酵素遺伝子 / アンモニア酸化酵素遺伝子 / 海洋環境 |
Outline of Final Research Achievements |
New nonmethane hyhdrocarbon assimilating marine bacteria were isolated and pmo-like genes were found in the isolates. Those pmo-like genes of marine strains were analyzed. Primers for pmo-like genes of non-methanotrophic bacteria were created. Those pmo-like genes in the environmental samples were amplified using the primers by PCR and a new group of genes in addition to the genes in the group of ethane- or ethylene- assimilating bacteria were detected.
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Report
(4 results)
Research Products
(9 results)
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[Presentation] 上越沖メタン湧出域を含む日本近海の海水中のメタン酸化細菌の分布2014
Author(s)
劉園, 逸見彰大, 阿久津祥吾, 四方麻妃, 塩谷達也, 江晨, 布施博之 , 寺原猛, 今田千秋, 帆秋利洋, 沖田紀子, 吉田光毅, 海野圭祐
Organizer
第6回メタンハイドレート総合シンポジウム講演集
Place of Presentation
東京
Year and Date
2014-12-03 – 2014-12-04
Related Report
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[Presentation] Methanotrophic Bacteria in Seawater around Japan.2014
Author(s)
Fuse H., Henmi A., Akutsu S., Shikata M., Shioya T., Jiang C. Liu Y., Terahara T., Imada C., Yoshida K., Okita N., Habe H
Organizer
Molecular Basis of Microbial One-Carbon Metabolism (Gordon Research Conferences)
Place of Presentation
MA, USA
Year and Date
2014-08-10 – 2014-08-15
Related Report
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