The biological meaning of neurite outgrowth via bisphenol A and its receptor

• Project/Area Number: 25340104
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental risk control and evaluation
• Research Institution: Kansai University
• Principal Investigator: SHIMOKE KOJI 関西大学, 化学生命工学部, 教授 (10351496)
• Co-Investigator(Kenkyū-buntansha): IKEUCHI Toshihiko 関西大学, 化学生命工学部, 教授 (20093362)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: bisphenol A / PC12細胞 / 大脳皮質神経細胞 / エピジェネティクス / 環境ホルモン / 生態評価 / 神経突起 / 神経細胞 / ビスフェノールA / 甲虫モデル / 極性 / 発生

Outline of Final Research Achievements

This research has been performed because of finding the neurite extending effect of bisphenol A(BPA), which belongs to endocrine disrupting chemicals. The research by others has been remitted to the sexual effect although ours focus on neurons. The results are that BPA induced neurite outgrowth with bipolarization on soma through histone H3 modification (acetylation) on specific lysine residue.

Research Products

• [Journal Article] Genomic control of upregulation of GRP78 expression for promotion of neurite elongation and attenuation of cell death via PKA-mediated signaling in PC12 cells (2015)
  • Author(s): R. Yamazoe, Y. Nishihata, K. Nakagawa, H. Aoyama, K. Shimoke
  • Journal Title: Clinical Pharmacology and Biopharmaceutics Volume: 4 Issue: 04 Pages: 150-154
  • DOI: 10.4172/2167-065x.1000150
  • NAID: 120006319645
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Endocrine disrupting chemicals - inducers of epigenetic gene expression and enhancers of cell death in neurons (2015)
  • Author(s): K. Shimoke
  • Journal Title: Journal of Bioengineering and Biomedical Sciences Volume: 6 Issue: 01
  • DOI: 10.4172/2155-9538.1000e122
  • NAID: 120006319643
  • Open Access / Acknowledgement Compliant
• [Journal Article] he histone deacetylase inhibitor trichostatin A induces neurite outgrowth in PC12 cells via the epigenetically regulated expression of the nur77 gene (2014)
  • Author(s): Tomioka T, Maruoka H, Kawa H, Yamazoe R, Fujiki D, Shimoke K, Ikeuchi T.
  • Journal Title: Neuroscience Research Volume: 88 Pages: 39-48
  • DOI: 10.1016/j.neures.2014.07.009
  • Peer Reviewed
• [Presentation] Forskolinによって誘導されるPC12細胞の神経突起伸長と最初期遺伝子nur77を介した発現機構の詳細 (2015)
  • Author(s): 島山恵利花, 津村風帆, 井戸大記, 丸岡弘規, 冨岡拓磨, 山添亮輔, 下家浩二
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートピアホテル（兵庫）
  • Year and Date: 2015-12-01
• [Presentation] nur77ファミリー遺伝子の発現はヒストン修飾によるエピジェネティックな分子制御機構を介している (2015)
  • Author(s): 山添亮輔, 山本宇晃, 冨岡拓磨, 丸岡弘規, 下家浩二
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートピアホテル（兵庫）
  • Year and Date: 2015-12-01
• [Presentation] Forskolinによる神経突起伸長を誘導するnur77遺伝子上流の転写活性制御機構 (2015)
  • Author(s): 谷尾啓介, 津村風帆, 島山恵利花, 山添亮輔, 丸岡弘規, 下家浩二
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートピアホテル（兵庫）
  • Year and Date: 2015-12-01
• [Presentation] Bisphenol Aによる神経突起伸長における細胞内分子機構の解析 (2015)
  • Author(s): 松浦玖実, 青山大輝, 藤枝聡志, 山添亮輔, 玄古宗一郎, 水井利幸, 小島正巳, 下家浩二
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートピアホテル（兵庫）
  • Year and Date: 2015-12-01
• [Presentation] Involvement of specific nur77 family genes during neurite outgrowth induced by forskolin and a histone deacetylase inhibitor in PC12 cells (2015)
  • Author(s): K. Shimoke, R. Yamazoe, T. Tomioka, K. Tsumura, Y. Nisihata, H. Maruoka, T. Ikeuchi
  • Organizer: 45th Annual Meeting of Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Relevance between the expression of nur family genes and the neurite outgrowth through the histone modification (2015)
  • Author(s): 山添亮輔, 西畑慶紀, 津村風帆, 島山恵利花, 冨岡拓磨, 丸岡弘規, 下家浩二
  • Organizer: 日本神経化学会
  • Place of Presentation: 大宮ソニックシティ（埼玉）
  • Year and Date: 2015-09-11
• [Presentation] Neurite outgrowth and bipolarization in PC12 cells and cerebral cortical neurons induced by a low concentration of bisphenol A (2015)
  • Author(s): 青山大輝, 藤枝聡志, 玄古宗一郎, 松浦玖実, 山添亮輔, 水井利幸, 小島正巳, 下家浩二
  • Organizer: 日本神経化学会
  • Place of Presentation: 大宮ソニックシティ（埼玉）
  • Year and Date: 2015-09-11
• [Presentation] 低濃度Bisphenol AによるPC12細胞と大脳皮質神経細胞への神経突起伸長への形態的変化作用 (2014)
  • Author(s): 青山大輝、篠崎遼太、玄古宗一郎、藤枝聡志、水井利幸、小島正己、下家浩二
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 各種HDAC阻害剤によるNur77遺伝子の発現上昇と神経突起伸長度の相関性の比較解析 (2014)
  • Author(s): 西畑慶紀、冨岡拓磨、丸岡弘規、上里新一、下家浩二
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] nur77ファミリー遺伝子の発現と神経突起伸長作用との関連性 (2014)
  • Author(s): 冨岡拓磨, 津村風帆, 西畑慶紀, 森田有貴, 山添亮輔, 丸岡弘規, 池内俊彦 , 下家浩二
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 京都国際会議場（京都府）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Bisphenol AによるPC12細胞の神経突起伸長におけるNGFとの形態的比較解析
  • Author(s): 青山大輝、豊田雄資、藤枝聡志、池内俊彦、下家浩二
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県）