A Study of Effect on Cardiac Pacemaker Control by Docosahexaenoic Acid

• Project/Area Number: 25350133
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Kagawa Prefectural College of Health Sciences
• Principal Investigator: YAMANUSHI TOMOKO 香川県立保健医療大学, 教養部, 准教授 (40382395)
• Co-Investigator(Kenkyū-buntansha): Kabuto Hideaki 香川県立保健医療大学, 教養部, 教授 (00321266) ; Hirakawa Eiichiro 香川県立保健医療大学, 保健医療学部, 教授 (60238342)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 機能性脂質 / DHA / 心臓拍動 / 洞房結節 / 心不全 / 心臓 / Δ6不飽和化酵素

Outline of Final Research Achievements

Five-week-old male rats induced right-sided congestive heart failure (CHF), were orally administered daily either 1g/kg body weight DHA-ethyl ester (DHA-Et) or equal volume of saline for 24 days. The rats were killed and dissected right ventricle (RV) and sinoatrial node (SAN). The quantitative PCR was used to measure the expression of transcripts of calcium handling proteins and sodium and potassium channels. In RV, by DHA-Et administration, abnormal expression of calcium handling proteins mRNA (Ca2+-ATPase 2a, Cav1.2, Cav3.1, ryanodine receptor 3 and inositol triphosphate receptor 2) was recovered. In SAN, this phenomenon was not observed. Administration of DHA-Et may improve abnormal Ca2+ handling through recovery of the expression of these proteins.

Research Products

• [Int'l Joint Research] マンチェスター大学(英国)
• [Journal Article] Effect of oral administration of DHA on the remodeling of calcium handling proteins in congestive heart failure in male rats (2015)
  • Author(s): Tomoko Yamanushi, Hideaki Kabuto, Kaoru Akiyama, Tadahiro Tsushima,and Yoshihisa Misawa
  • Journal Title: FASEB J April Volume: 29 (1 Supplement)
  • Peer Reviewed
• [Presentation] DHA経口摂取による右鬱血性心不全ラット心筋細胞イオンチャネル発現の変化 (2015)
  • Author(s): 山主智子、加太英明、小河佳織、高山房子、対馬忠広、三澤嘉久、万倉三正
  • Organizer: 日本脂質栄養学会第24回大会
  • Place of Presentation: 佐賀県佐賀市 ホテルグランデはがくれ
  • Year and Date: 2015-08-28
• [Presentation] 鬱血性心不全ラット洞房結節のカルシウム制御遺伝子発現異常に対するDHA経口摂取の影響 (2015)
  • Author(s): 山主智子、加太英明、小河佳織、高山房子、対馬忠広、三澤嘉久、万倉三正
  • Organizer: 第70会日本栄養・食糧学会大会
  • Place of Presentation: 兵庫県西宮市 武庫川女子大学
  • Year and Date: 2015-05-13
• [Presentation] Effect of oral administration of DHA on the remodeling of calcium handling proteins in congestive heart failure in male rats (2015)
  • Author(s): Tomoko T. Yamanushi, Hideaki Kabuto, Kaoru Akiyama, Tadahiro Tsushima and Yoshihisa Misawa
  • Organizer: EB2015 (Annual meeting of American Society for Nutrition)
  • Place of Presentation: Boston
  • Year and Date: 2015-03-28 – 2015-04-01