| Project/Area Number |
25350169
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | Nagasaki International University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OISO Shigeru 長崎国際大学, 薬学部, 准教授 (40513106)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | グレリン / 脂肪酸 / オレイン酸 / AGS-GHRL8細胞 / オクタン酸 / AGS-GHRL8 / ウルソール酸 / 抗肥満 |
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| Outline of Final Research Achievements |
An active ghrelin secreting cell line, AGS-GHRL8, was established by transfecting ghrelin cDNA into AGS cells originating from human gastric carcinoma. Culture of AGS-GHRL8 cells in the presence of octanoic acid caused the secretion of octanoylated (active) ghrelin. To explore the molecules suppressing active ghrelin secretion, we examined the potential of several food ingredients using AGS-GHRL8 cells. Subsequently, we observed that in addition to common dietary fatty acids such as stearic acid, oleic acid, linoleic acid, and α-linolenic acid, a range of plant-derived molecules such as ursolic acid, corosolic acid, glycyrrhetinic acid and epigallocatechin gallate suppressed active ghrelin secretion. Treatment of mice with oleic acid for two weeks lowered the circulating levels of active ghrelin. These results indicate the promising use of oleic acid as an anti-obesity agent and demonstrate the utility of AGS-GHRL8 cells in screening candidate inhibitors of active ghrelin production.
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