Identification of the specific receptors for the nanomaterials

• Project/Area Number: 25350524
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Shinshu University
• Principal Investigator: USUI Yuki 信州大学, 医学部附属病院, 特任研究員 (00467169)
• Co-Investigator(Kenkyū-buntansha): HANIU Hisao 信州大学, 学術研究院医学系, 准教授 (30252050) ; TSUKAHARA Tamotsu 長崎大学, 医歯(薬)学総合研究科, 准教授 (00529943)
• Co-Investigator(Renkei-kenkyūsha): SAITO Naoto 信州大学, 学術研究院保健学系, 教授 (80283258) ; KOBAYASHI Shinsuke 信州大学, 医学部附属病院, 医員 (40624705) ; NOMURA Hiroki 信州大学, 医学部附属病院, 医員 (40646543)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 生体物性 / ナノマテリアル / カーボンナノチューブ / エンドサイトーシス

Outline of Final Research Achievements

The culture medium composition affected the proteins that are expressed on the cytoplasmic membrane, which influence the biological response to MWCNTs, especially its cellular uptake. MWCNTs were positively taken up by nonphagocytic cells, and their cytotoxicity was closely related to these three endocytosis pathways (clathrin-mediated, caveolae-mediated, and macropinocytosis). ITGB1 and MARCO may related to receptor-mediated endocytosis of MWCNTs in the nonphagocytic cells. The MWCNTs-endocytosed cells induced LC3B expression and induced cell growth inhibition.

Research Products

• [Journal Article] The role of autophagy as a mechanism of toxicity induced by multi-walled carbon nanotubes in human lung cells. (2015)
  • Author(s): Tsukahara T, Matsuda Y, Haniu H.
  • Journal Title: Int J Mol Sci. Volume: 16 Issue: 1 Pages: 40-48
  • DOI: 10.3390/ijms16010040
  • NAID: 120007110640
  • Peer Reviewed / Open Access
• [Journal Article] Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells. (2015)
  • Author(s): Maruyama K, Haniu H, Saito N, Matsuda Y, Tsukahara T, Kobayashi S, Nomura H, Tanaka M, Aoki K, Takanashi S, Okamoto M, Kato H.
  • Journal Title: BioMed Res Int. Volume: 2015 Pages: 793186-793186
  • NAID: 120007110651
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells (2014)
  • Author(s): Haniu H, Saito N, Matsuda Y, Tsukahara T, Usui Y, Maruyama K, Takanashi S, Aoki K, Kobayashi S, Nomura H, Tanaka M, Okamoto M, Kato H.
  • Journal Title: Int J Nanomedicine. Volume: 9 Pages: 1979-90
  • NAID: 120007110603
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Role of Autophagy as a Mechanism of Toxicity Induced by Multi-Walled Carbon Nanotubes in Human Lung Cells. (2014)
  • Author(s): Tsukahara T, Matsuda Y, Usui Y, Haniu H.
  • Journal Title: Int J Mol Sci. Volume: 16 Pages: 40-48
  • NAID: 120007110640
  • Peer Reviewed / Open Access
• [Journal Article] Culture medium type affects endocytosis of multi-walled carbon nanotubes in BEAS-2B cells and subsequent biological response (2013)
  • Author(s): Haniu H, Saito N, Matsuda Y, Tsukahara T, Maruyama K, Usui Y, Aoki K, Takanashi S, Kobayashi S, Nomura H, Okamoto M, Shimizu M, Kato H
  • Journal Title: Toxicol in Vitro Volume: 27 Issue: 6 Pages: 1679-1685
  • DOI: 10.1016/j.tiv.2013.04.012
  • NAID: 120007110519
  • Peer Reviewed
• [Journal Article] Highly purified, multi-wall carbon nanotubes induce light-chain 3B expression in human lung cells. (2013)
  • Author(s): Tsukahara T, Matsuda Y, Usui Y, Haniu H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 440 Issue: 2 Pages: 348-53
  • DOI: 10.1016/j.bbrc.2013.09.089
  • NAID: 120007100190
  • Peer Reviewed
• [Presentation] Effect of multi-walled carbon nanotubes in three different dispersants on MC3T3-E1 cell line proliferation. (2015)
  • Author(s): Haniu H.
  • Organizer: 5th International Conference on Nanotek & Expo
  • Place of Presentation: San Antonio, USA
  • Year and Date: 2015-11-16
  • Int'l Joint Research
• [Presentation] Evaluation of Multi-walled Carbon Nanotubes Using the MC3T3-E1 Cell Line. (2015)
  • Author(s): Haniu H, Saito N, Maruyama K, Matsuda N, Tanaka M, Aoki K, Okamoto M, Kobayashi S, Nomura H, Takizawa T, Ohishi A, Usui Y, Kato H.
  • Organizer: TechConnect World Innovation Conference & Expo-2015
  • Place of Presentation: Washinton, DC, USA
  • Year and Date: 2015-06-14
  • Int'l Joint Research
• [Presentation] BEAS-2B細胞の多層カーボンナノチューブの取り込み (2014)
  • Author(s): 丸山佳与，羽二生久夫，薄井雄企，青木薫，高梨誠司，岡本正則，小林伸輔，野村博紀，田中学，松田佳和，加藤博之，齋藤直人
  • Organizer: 第41回日本毒性学会学術年会
  • Place of Presentation: 神戸・神戸コンベンションセンター
  • Year and Date: 2014-07-02 – 2014-07-04
• [Presentation] Endocytosis of MWCNT in BEAS-2B cells are affected by the culture medium type. (2013)
  • Author(s): Hisao Haniu, Naoto Saito, Yoshikazu Matsuda, Kayo Maruyama, Tamotsu Tsukahara, Yuki Usui, Kaoru Aoki, Seiji Takanashi, Shinsuke Kobayashi, Hiroki Nomura, Masanori Okamoto, Masayuki Shimizu, Hiroyuki Kato.
  • Organizer: 6th International Symposium on Nanotechnology, Occupational and Environmental Health
  • Place of Presentation: 愛知県名古屋市
• [Presentation] 培養液による多層カーボンナノチューブのBEAS-2B細胞におけるバイオレ スポンスへの影響
  • Author(s): 羽二生久夫、齋藤直人、松田佳和、丸山佳与、薄井雄企、青木薫、高梨誠司、 小林伸輔、野村博紀、岡本正則、清水政幸、加藤博之
  • Organizer: 第40回日本毒性学会学術年会
  • Place of Presentation: 千葉県千葉市