Visualization of in vivo mouse coronary arteries using high intensity quasi-monochromatic x-rays generated by an x-ray mirror

• Project/Area Number: 25350547
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Japan Synchrotron Radiation Research Institute
• Principal Investigator: Keiji Umetani 公益財団法人高輝度光科学研究センター, 利用研究促進部門, 主幹研究員 (50344396)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: マウス冠状動脈撮影 / 血管造影 / 放射光 / Ｘ線ミラー / 準単色Ｘ線 / 心臓疾患診断 / マウス冠状動脈 / 心臓疾患

Outline of Final Research Achievements

Rat coronary microangiography at the SPring-8 synchrotron radiation facility has been applied to numerous pre-clinical studies by visualization of fine angioarchitectures comparable with those of human angiographic anatomy. Next, we undertook new studies on visualization of mouse coronary arteries because a lot of disease model mice can be produced by gene recombination. We developed a new high-speed imaging system using a combination of high intensity quasi-monochromatic x-rays generated by an x-ray mirror and a sub-millisecond precision x-ray shutter because mouse bodies are smaller than those of rats and mouse heart rates are faster than those of rats. The new system will be an effective tool for pharmacometrics and studies on disease mechanisms using various types of disease model mice.

Research Products

• [Journal Article] Sympathetic hyper-excitation in obesity and pulmonary hypertension - physiological relevance to the 'Obesity Paradox' (2016)
  • Author(s): Crystal Diong, Peter Jones, Hirotsugu Tsuchimochi, Emily Gray, Gillian Hughes, Tadakatsu Inagaki, Carol Bussey, Yutaka Fujii, Keiji Umetani, Mikiyasu Shirai, Daryl Schwenke
  • Journal Title: International Journal of Obesity Volume: 40 Issue: 6 Pages: 938-946
  • DOI: 10.1038/ijo.2016.33
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ghrelin promotes functional angiogenesis in a mouse model of critical limb ischemia through activation of pro-angiogenic microRNAs (2016)
  • Author(s): Rajesh Katare, Shruti Rawal, Pujika E Munasinghe, Parul Dixit, Hirotsugu Tsuchimochi, Tadakatsu Inagaki, Yutaka Fujii, Keiji Umetani, Kenji Kangawa, Mikiyasu Shirai, Daryl Schwenke
  • Journal Title: Endocrinology Volume: 157 Issue: 2 Pages: 432-445
  • DOI: 10.1210/en.2015-1799
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Altered blood flow in cerebral perforating arteries of rat models of diabetes: A synchrotron radiation microangiographic study toward clinical evaluation of white matter hyperintensities (2015)
  • Author(s): Naoto Fukuyama, Yuko Tsukamoto, Shunya Takizawa, Yoshimori Ikeya, Toshiharu Fujii, Yoshiro Shinozaki, Yoko Takahari, Noboru Kawabe, Noriaki Wakana, Keiji Umetani, Kikue Todoroki, Sayato Fukui, Chiharu Tanaka, Etsuro Tanaka, Hidezo Mori
  • Journal Title: Geriatrics & Gerontology International Supplement Volume: 15 Issue: S1 Pages: 74-80
  • DOI: 10.1111/ggi.12658
  • Peer Reviewed
• [Journal Article] Development of 36M-Pixel Micro-CT Using Digital Single-Lens Reflex Camera (2015)
  • Author(s): Keiji Umetani, Yoshiki Kawata, Noboru Niki, Harumi Itoh
  • Journal Title: Proceedings of the 2015 IEEE International Conference on Imaging Systems & Techniques (IST) Volume: - Pages: 11-15
  • DOI: 10.1109/ist.2015.7294461
  • Peer Reviewed
• [Journal Article] Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia (2015)
  • Author(s): Hisashi Nagai, Ichiro Kuwahira, Daryl O. Schwenke, Hirotsugu Tsuchimochi, Akina Nara, Sayoko Ogura, Takashi Sonobe, Tadakatsu Inagaki, Yutaka Fujii, Rutsuko Yamaguchi, Lisa Wingenfeld, Keiji Umetani, Tatsuo Shimosawa, Kenichi Yoshida, Koichi Uemura, James T. Pearson, Mikiyasu Shirai
  • Journal Title: PLoS ONE Volume: 10 Issue: 7 Pages: 1-20
  • DOI: 10.1371/journal.pone.0131923
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of Fingertip Synchrotron Radiation Microangiography toward Clinical Prediction of Diabetic Microangiopathy (2015)
  • Author(s): Toshiharu Fujii, Naoto Fukuyama, Yoshimori Ikeya, Chiharu Tanaka, Yoshiro Shinozaki, Kazuhito Fukushima, Keiji Umetani, Yuji Ikari, Hidezo Mori
  • Journal Title: The Tokai Journal of Experimental and Clinical Medicine Volume: 40 Pages: 1-7
  • Peer Reviewed / Open Access
• [Journal Article] Microstructure analysis of the pulmonary acinus using a synchrotron radiation CT (2015)
  • Author(s): Yoshitaka Tokumoto, Koichi Minami, Yoshiki Kawata, Noboru Niki, Keiji Umetani, Yasutaka Nakano, Hiroaki Sakai, Hironobu Ohmatsu, Harumi Itoh
  • Journal Title: Proc. SPIE 9417 Volume: 9417 Pages: 94172P-94172P
  • DOI: 10.1117/12.2081357
  • NAID: 40020960877
  • Peer Reviewed
• [Journal Article] Cell-sheet Therapy With Omentopexy Promotes Arteriogenesis and Improves Coronary Circulation Physiology in Failing Heart. (2015)
  • Author(s): Kainuma S, Miyagawa S, Fukushima S, Pearson J, Chen YC, Saito A, Harada A, Shiozaki M, Iseoka H, Watabe T, Watabe H, Horitsugi G, Ishibashi M, Ikeda H, Tsuchimochi H, Sonobe T, Fujii Y, Naito H, Umetani K, Shimizu T, Okano T, Kobayashi E, Daimon T, Ueno T, Kuratani T, Toda K, Takakura N, Hatazawa J, Shirai M, Sawa Y.
  • Journal Title: Mol Ther Volume: 23 Issue: 2 Pages: 374-386
  • DOI: 10.1038/mt.2014.225
  • Peer Reviewed
• [Journal Article] Beta2-adrenergic receptor-dependent attenuation of hypoxic pulmonary vasoconstriction prevents progression of pulmonary arterial hypertension in intermittent hypoxic rats (2014)
  • Author(s): Hisashi Nagai, Ichiro Kuwahira, Daryl O. Schwenke, Hirotsugu Tsuchimochi, Akina Nara, Tadakatsu Inagaki, Sayoko Ogura, Yutaka Fujii, Keiji Umetani, Tatsuo Shimosawa, Kenichi Yoshida, James T. Pearson, Koichi Uemura, Mikiyasu Shirai
  • Journal Title: PLOS ONE Volume: 10 Issue: 10 Pages: 1-10
  • DOI: 10.1371/journal.pone.0110693
  • Peer Reviewed / Open Access
• [Journal Article] Pulmonary vascular tone is dependent on the central modulation of sympathetic nerve activity following chronic intermittent hypoxia (2014)
  • Author(s): Mikiyasu Shirai, Hirotsugu Tsuchimochi, Hisashi Nagai, Emily Gray, James Todd Pearson, Takashi Sonobe, Misa Yoshimoto, Tadakatsu Inagaki, Yutaka Fujii, Keiji Umetani, Ichiro Kuwahira, Daryl Schwenke
  • Journal Title: Basic Research in Cardiology Volume: 109 Issue: 5 Pages: 432-432
  • DOI: 10.1007/s00395-014-0432-y
  • Peer Reviewed
• [Journal Article] Microstructure analysis of the pulmonary lung of the secondary lobulus by a synchrotron radiation CT (2014)
  • Author(s): Yasunori Fukuoka, Yoshiki Kawata, Noboru Niki, Keiji Umetani, Yasutaka Nakano, Noriyuki Moriyama, Hironobu Ohmatsu, Harumi Itoh
  • Journal Title: Proc. SPIE Medical Imaging Volume: 9035 Pages: 90352F-90352F
  • DOI: 10.1117/12.2043454
  • Peer Reviewed
• [Journal Article] Phase contrast portal imaging for image-guided microbeam radiation therapy (2014)
  • Author(s): Keiji Umetani, Takeshi Kondoh
  • Journal Title: Proc. SPIE Medical Imaging Volume: 9033 Pages: 90335P-90335P
  • DOI: 10.1117/12.2043216
  • Peer Reviewed
• [Journal Article] Phase contrast portal imaging using synchrotron radiation (2014)
  • Author(s): Keiji Umetani, Takeshi Kondoh
  • Journal Title: Review of Scientific Instruments Volume: 85 Issue: 7
  • DOI: 10.1063/1.4885755
  • Peer Reviewed
• [Journal Article] The Serotonin Pathway-A Therapeutic Target For Pulmonary Hypertension? Assessing Vascular Function Using Synchrotoron Radiation Microangiography. (2013)
  • Author(s): Gray EA, Tsuchimochi H, Pearson JT, Sonobe T, Fujii Y, Yoshimoto M, Umetani K, Shirai M, Schwenke DO.
  • Journal Title: J Synchrotron Radiat Volume: 20(5) Issue: 5 Pages: 756-764
  • DOI: 10.1107/s0909049513021213
  • Peer Reviewed
• [Journal Article] Acute Rho-kinase inhibition improves coronary dysfunction in vivo, in th e early diabetic microcirculation. (2013)
  • Author(s): Pearson JT, Jenkins Mi. Edgley AJ, Sonobe T, Joshi M. Waddingham MT, Fujii V. Schwenkc DO, Tsuchi mochi H. Yoshimoto M, Umetani K, Kelly DJ, Shirai M.
  • Journal Title: Cardiovasc Diabetol Volume: 12 Issue: 1 Pages: 111-124
  • DOI: 10.1186/1475-2840-12-111
  • Peer Reviewed
• [Journal Article] Chronic oxidative-nitrosative stress impairs coronary vasodilation in metabolic syndrome model rats (2013)
  • Author(s): Kagota S, Maruyama K, Tada Y, Fukushima K, Umetani K, Wakuda H, Shinozuka K.
  • Journal Title: Microvascular Research Volume: in press Pages: 70-78
  • DOI: 10.1016/j.mvr.2013.04.001
  • Peer Reviewed
• [Journal Article] Impaired pulmonary blood flow distribution in congestive heart failure assessed using synchrotron radiation microangiography. (2013)
  • Author(s): Shirai M, Beard M, Pearson JT, Son obe T, Tsuchimochi H, Fujii Y, Gray E, Umetani K. Schwenke DO.
  • Journal Title: J Synchrotron Radiat Volume: 20(3) Issue: 3 Pages: 441-448
  • DOI: 10.1107/s0909049513007413
  • Peer Reviewed
• [Journal Article] Rat coronary microangiography system for preclinical imaging using synchrotron radiation (2013)
  • Author(s): Keiji Umetani, James T. Pearson, Daryl O. Schwenke, Mikiyasu Shirai
  • Journal Title: Proc. SPIE Medical Imaging Volume: 8668 Pages: 866864-866864
  • DOI: 10.1117/12.2006570
  • Peer Reviewed
• [Journal Article] Stochastic tracking of small pulmonary vessels in human lung alveolar walls using synchrotron radiation micro CT images (2013)
  • Author(s): Yoshiki Kawata, Noboru Niki, Keiji Umetani, Yasutaka Nakano, Hironobu Ohamatsu, Noriyuki Moriyama, Harumi Itoh
  • Journal Title: Proc. SPIE Medical Imaging Volume: 8672 Pages: 867211-867211
  • DOI: 10.1117/12.2006895
  • Peer Reviewed
• [Presentation] Development of 36M-Pixel Micro-CT Using Digital Single-Lens Reflex Camera (2015)
  • Author(s): Keiji Umetani, Yoshiki Kawata, Noboru Niki, Harumi Itoh
  • Organizer: Ｔhe 2015 IEEE International Conference on Imaging Systems & Techniques (IST)
  • Place of Presentation: Macau, China
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] Microstructure analysis of the pulmonary acinus using a synchrotron radiation CT (2015)
  • Author(s): Yoshitaka Tokumoto, Koichi Minami, Yoshiki Kawata, Noboru Niki, Keiji Umetani, Yasutaka Nakano, Hiroaki Sakai, Hironobu Ohmatsu, Harumi Itoh
  • Organizer: SPIE Medical Imaging
  • Place of Presentation: Ｒｅｎａｉｓｓａｎｃｅ Orlando (Orlando, Florida, USA)
  • Year and Date: 2015-02-21 – 2015-02-26
• [Presentation] デジタル一眼レフカメラを使った高解像度Ｘ線検出器の開発 (2015)
  • Author(s): 梅谷啓二、河田佳樹、仁木 登
  • Organizer: 第２８回日本放射光学会年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス（滋賀県草津市）
  • Year and Date: 2015-01-10 – 2015-01-12
• [Presentation] デジタル一眼レフカメラを使った高解像度マイクロＣＴの開発 (2014)
  • Author(s): 梅谷 啓二、河田 佳樹、仁木 登
  • Organizer: 2014年映像情報メディア学会年次大会
  • Place of Presentation: 大阪大学吹田キャンパス（大阪府吹田市）
  • Year and Date: 2014-08-31 – 2014-09-02
• [Presentation] 高解像度マイクロＣＴ用の３６Ｍ画素Ｘ線検出器の開発 (2014)
  • Author(s): 梅谷啓二 河田佳樹 福岡泰規 徳元祥貴 仁木 登
  • Organizer: 第３３回日本医用画像工学会大会
  • Place of Presentation: 東京慈恵会医科大学（東京都港区）
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] Phase contrast portal imaging for image-guided microbeam radiation therapy (2014)
  • Author(s): K. Umetani, T. Kondoh
  • Organizer: SPIE Medical Imaging
  • Place of Presentation: San Diego, California, USA
• [Presentation] Microstructure analysis of the pulmonary lung of the secondary lobulus by a synchrotron radiation CT (2014)
  • Author(s): Y. Fukuoka, Y. Kawata, N. Niki, K. Umetani, Y. Nakano, N. Moriyama, H. Ohmatsu, H. Itoh
  • Organizer: SPIE Medical Imaging
  • Place of Presentation: San Diego, California, USA
• [Presentation] マイクロビーム放射線治療のための屈折コントラスト法での照射野確認画像の撮影 (2014)
  • Author(s): 梅谷啓二、近藤 威
  • Organizer: 日本放射光学会
  • Place of Presentation: 広島県広島市
• [Presentation] 放射光による放射線治療のための屈折コントラスト法での照射野確認画像撮影 (2013)
  • Author(s): 梅谷啓二、近藤 威
  • Organizer: 映像情報メディア学会
  • Place of Presentation: 東京都新宿区
• [Presentation] 放射光生体顕微法によるマウス冠状動脈撮影装置の開発 (2013)
  • Author(s): 梅谷啓二、James T. Pearson、Daryl O. Schwenke、白井幹康
  • Organizer: 日本医用画像工学会
  • Place of Presentation: 東京都江東区
• [Remarks] 公益財団法人 高輝度光科学研究センター(JASRI/SPring-8) 利用研究促進部門
  • URL: http://rud.spring8.or.jp/
• [Remarks] 大型放射光施設 SPring-8 研究事例&研究成果
  • URL: http://www.spring8.or.jp/ja/science/
• [Remarks] 大型放射光施設 SPring-8 利用事例＆研究成果
  • URL: http://www.spring8.or.jp/ja/science/