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Study on microfluidic devices for isolation of circulating tumor cells expressing a variety of surface markers

Research Project

Project/Area Number 25350582
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Medical systems
Research InstitutionToyama Industrial Technology Center,

Principal Investigator

Ohnaga Takashi  富山県工業技術センター, その他部局等, 研究員 (10416133)

Co-Investigator(Kenkyū-buntansha) TSUKADA KAZUHIRO  富山大学, 大学院医学薬学研究部, 教授 (90171967)
SHIMADA YUTAKA  京都大学, 薬学研究科, 客員教授 (30216072)
Co-Investigator(Renkei-kenkyūsha) KISHI HIROYUKI  富山大学, 大学院医学薬学研究部, 准教授 (60186210)
OBATA TSUTOMU  富山県工業技術センター, 中央研究所, 研究員 (30416143)
TAKATA KOUJI  富山県工業技術センター, 機械電子研究所, 研究員 (40530621)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords血中循環腫瘍細胞 / 癌 / 抗体 / EGFR / EpCAM / CTCチップ / マイクロ流体デバイス
Outline of Final Research Achievements

We have developed polymeric microfluidic devices for isolation of circulating tumor cells (CTCs). The device targeted EpCAM expressed on cancer cells and captured them with anti-EpCAM antibody immobilized on the device. However, this device has been known to be of no use for cancer cells expressing little EpCAM and therefore we took account of other targets of cell surface makers and incorporated antibodies against them into the device in this study. Since EGFR is known to be overexpressed in many kinds of cancer, we used anti-EGFR antibodies for the capture.
As a result, the anti-EGFR antibody Cetuximab was shown to efficiently capture esophageal cancer cells of KYSE series (EGFR changes from 60,000 to 12,000,000 receptors/cell) despite of low-efficient capture by the other anti-EGFR antibodies used in this study. Cetuximab also captured well breast cancer cells of MDA-MB-231 that is known to hard to capture them with anti-EpCAM antibodies.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Capture of esophageal and breast cancer cells with polymeric microfluidic devices for CTC isolation2016

    • Author(s)
      TAKASHI OHNAGA, YUTAKA SHIMADA, KOJI TAKATA, TSUTOMU OBATA, TOMOYUKI OKUMURA, TAKUYA NAGATA, HIROYUKI KISHI, ATSUSHI MURAGUCHI and KAZUHIRO TSUKADA
    • Journal Title

      MOLECULAR AND CLINICAL ONCOLOGY

      Volume: 4 Issue: 4 Pages: 599-602

    • DOI

      10.3892/mco.2016.734

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 新規血液中浮遊癌細胞(CTC)捕捉システムによる乳癌患者末梢血液中のCTC同定と抗癌剤感受性予測2015

    • Author(s)
      長田拓哉、大永崇、呂暁龍、渡辺徹、平野勝久、奥村知之、塚田一博
    • Journal Title

      癌と化学療法

      Volume: 42 Pages: 1240-1242

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 新型CTC チップを用いた循環神経芽腫細胞の捕捉は切除適応を決める診断ツールとなりうる2015

    • Author(s)
      横堀武彦、大竹紗弥香、大永崇、鈴木信、浅尾高行、桑野博行、西山正彦
    • Organizer
      日本癌学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 血中循環腫瘍細胞をマーカー依存及び非依存で分離回収する樹脂マイクロ流体チップ2015

    • Author(s)
      高田耕児、大永崇、長田拓哉、嶋田裕、塚田一博
    • Organizer
      日本癌学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] ポリマー製のマイクロ流体装置を用いたCTC の検出2015

    • Author(s)
      呉一眞、細谷理樹、小見山博光、冨木裕一、落合匠、大永崇、坂本一博
    • Organizer
      日本癌学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Capture of cancer cells expressing EGFR with polymeric microfluidic devices for CTC isolation2014

    • Author(s)
      大永 崇
    • Organizer
      日本癌学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞表面マーカーがCTC 単離用ポリマーマイクロ流体デバイスの捕捉効率に及ぼす影響

    • Author(s)
      大永 崇
    • Organizer
      日本癌学会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 微小体の封入、回収方法及びそれに用いられる封入用液体2014

    • Inventor(s)
      大永 崇
    • Industrial Property Rights Holder
      大永 崇
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-031587
    • Filing Date
      2014
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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