Comprehensive analysis of gene expression induced by muscle contraction in three-dimensional tissue-engineered muscle.

• Project/Area Number: 25350830
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Sports science
• Research Institution: Osaka Institute of Technology
• Principal Investigator: Nakamura Tomohiro 大阪工業大学, 工学部, 教授 (30217872)
• Co-Investigator(Kenkyū-buntansha): Fujisato Toshia 大阪工業大学, 工学部, 教授 (60270732)
• Co-Investigator(Renkei-kenkyūsha): Okuzaki Daisuke 大阪大学, 微生物病研究所, 助教 (00346131)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ３次元培養筋 / 網羅的解析 / マイオカイン / 三次元培養筋 / マイクロアレイ / 三次元培養骨格筋 / 電気刺激 / バイオインフォマテックス / 身体活動意義

Outline of Final Research Achievements

The aim of this study is to elucidate a novel role of muscle contraction by comprehensive gene analysis using three-dimensional tissue-engineered muscle.
Comprehensive gene analysis indicated that many genes encoding secretory proteins upregulated with muscle contraction. Furthermore, a gene associated feeding behavior included within the gene pool. Taken together, It is likely that skeletal muscles actively communicate and regulate remote organs through the production of secretory proteins by muscle contraction.

Research Products

• [Presentation] 三次元培養筋を利用した筋収縮依存的に転写誘導される遺伝子群の網羅的解析 (2015)
  • Author(s): 中村友浩、高木空、松本彰宏、秋本崇之、奥崎大介、藤里俊哉
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] 三次元培養筋を利用した新規マイオカイン遺伝子の探索 (2015)
  • Author(s): 中村友浩、高木空、奥崎大介、秋本崇之、藤里俊哉
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山
  • Year and Date: 2015-09-18
• [Presentation] 温熱刺激が３次元培養筋の収縮機能に及ぼす影響 (2015)
  • Author(s): 高木空、秋本崇之、藤里俊哉、中村友浩
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山
  • Year and Date: 2015-09-18
• [Presentation] The development of fatigue model in a tissue-engineered muscle. (2015)
  • Author(s): T.Nakamura, S.Takagi, T.Akimoto, T.Kamon, T.Fujisato.
  • Organizer: ECSS2015
  • Place of Presentation: Malmo,Sweden
  • Year and Date: 2015-06-23
  • Int'l Joint Research
• [Presentation] 三次元培養筋の構築と生理学的応用. (2015)
  • Author(s): 中村友浩、高木空、掃部貴文、秋本崇之、藤里俊哉
  • Organizer: 第３回 骨格筋生物学研究会.
  • Place of Presentation: 東北大学星陵キャンパス
  • Year and Date: 2015-03-06
• [Presentation] Effect of heat stimulation on contractile properties of tissue-engineered skeletal muscle (2014)
  • Author(s): Shunya TAKAGI, Takafumi KAMON, Tomohiro NAKAMURA, Toshia FUJISATO
  • Organizer: 2014 TERMIS‐AP
  • Place of Presentation: EXCO, Daegu, South Korea
  • Year and Date: 2014-09-27
• [Presentation] 三次元培養骨格筋を用いた疲労モデルの検討 (2014)
  • Author(s): 高木空、秋本崇之、藤里俊哉、中村友浩
  • Organizer: 第６９回 日本体力医学会
  • Place of Presentation: 長崎大学文教キャンパス
  • Year and Date: 2014-09-20
• [Presentation] コラーゲンと筋細胞を用いた 3 次元培養骨格 筋における温熱刺激応答の評価 (2014)
  • Author(s): 高木空, 中村友浩, 藤里 俊哉
  • Organizer: 平成２６年度繊維学会年次大会
  • Place of Presentation: タワーホール船堀 (江戸川区総合区民ホール)
  • Year and Date: 2014-06-13
• [Presentation] Effect of Electrical Stimulation of Tissue-Engineered Skeletal Muscle. (2013)
  • Author(s): Takagi S, Hatoma S, Kamon T, Nakamura T, Fujisato T
  • Organizer: TERMIS Americas Conference 2013
  • Place of Presentation: Atlanta, USA
• [Book] 運動生理学２０講 第三版 (2015)
  • Author(s): 中村友浩（第７講）；勝田茂、征矢英昭編
  • Total Pages: 187
  • Publisher: 朝倉書店