Inhibiting effect of blood flow restriction on skeletal muscle atrophy and investigation of related genes

• Project/Area Number: 25350899
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Juntendo University
• Principal Investigator: SAKURABA KEISHOKU 順天堂大学, スポーツ健康科学部, 教授 (50175460)
• Co-Investigator(Kenkyū-buntansha): KAKEI Saori 順天堂大学, 大学院医学(系)研究科(研究院), 特任助教 (00450560)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 血流制限 / 筋萎縮 / 遺伝子発現

Outline of Final Research Achievements

Expression levels of genes influencing skeletal muscle protein synthesis and breakdown were analyzed after immobilizing the human lower extremity in a cast as a model of muscle atrophy and weakness to clarify the mechanisms of the preventing effect of repeated local blood flow restriction on muscle atrophy and weakness. It was suggested that the regulation of gene expression level such as MuRF1, FoxO3, p70S6K may be involved in the mechanisms.

Research Products

• [Journal Article] Increased intramyocellular lipid/impaired insulin sensitivity is associated with altered lipid metabolic genes in muscle of high responders to a high-fat diet (2016)
  • Author(s): 1.Kakehi S, Tamura Y, Takeno K, Sakurai Y, Kawaguchi M, Watanabe T, Funayama T, Sato F, Ikeda S, Kanazawa A, Fujitani Y, Kawamori R, Watada H
  • Journal Title: Am J Physiol Endocrinol Metab Volume: 310(1)
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] TBC1D4/AS160のSNPは骨格筋インスリン抵抗性、2型糖尿病を規定する (2015)
  • Author(s): 筧 佐織、田村 好史
  • Journal Title: 内分泌・糖尿病・代謝内科 Volume: 41(1) Pages: 59-62
  • NAID: 40020553974
• [Journal Article] Effects of sitagliptin on ectopic fat contents and glucose metabolism in type 2 diabetic patients with fatty liver: A pilot study (2014)
  • Author(s): Watanabe T, Tamura Y, Kakehi S, Funayama T, Takeno K, Kawaguchi M, Yamamoto L, Sato F, Ikeda S, Taka H, Fujimura T, Fujitani Y, Kawamori R, Watada H
  • Journal Title: J Diabetes Invest Volume: Mar;6(2) Issue: 2 Pages: 164-72
  • DOI: 10.1111/jdi.12262
  • Peer Reviewed / Open Access
• [Journal Article] Association between expression of FABPpm in skeletal muscle and insulin sensitivity in intramyocellular-lipid-accumulated non-obese men (2014)
  • Author(s): Kawaguchi M, Tamura Y, Kakehi S, Takeno K, Sakurai Y, Watanabe T, Funayama T, Sato F, Ikeda S, Ogura Y, Saga N, Naito H, Fujitani Y, Kanazawa A, Kawamori R, Watada
  • Journal Title: J Clin Endocrinol Metab Volume: Sep;99(9) Issue: 9 Pages: 3343-52
  • DOI: 10.1210/jc.2014-1896
  • Peer Reviewed
• [Journal Article] Effect of Eccentric Contractions of Elbow Flexor on Bone Formation and Resorption Markers. (2014)
  • Author(s): Tsuchiya Y, Sakuraba K, Ochi E
  • Journal Title: The Journal of Sports Medicine and Physical Fitness Volume: in press
  • Peer Reviewed
• [Journal Article] Isokinetic strength and anaerobic / intermittent capacity of Japanese lacrosse players (2013)
  • Author(s): Tsuchiya Y, Ochi E, Sakuraba K, Kikuchi N, Hwang I
  • Journal Title: Isokinetics and Exercise Science Volume: 21 Pages: 77-82
  • Peer Reviewed
• [Journal Article] 骨格筋における異所性脂肪とインスリン抵抗性 (2013)
  • Author(s): 田村好史、筧佐織、竹野景海
  • Journal Title: 肥満研究 Volume: 19 Pages: 175-180
• [Presentation] 高脂肪・不活動による骨格筋インスリン抵抗性惹起メカニズム (2015)
  • Author(s): 5.筧 佐織、田村 好史、池田 真一、加賀 直子、河盛 隆造、綿田 裕孝
  • Organizer: 分子糖尿病学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2015-12-05
• [Presentation] 24時間の脚ギプス固定は、骨格筋細胞内のジアシルグリセロールを増加させ、インスリン抵抗性を惹起する (2015)
  • Author(s): 4.筧 佐織、田村 好史、池田 真一、河盛 隆造、綿田 裕孝
  • Organizer: 第70回体力医学会大会
  • Place of Presentation: 和歌山
  • Year and Date: 2015-09-18
• [Presentation] Intramyocellular lipid accumulation after high-fat diet is associated with the gene expression involved in lipid metabolism in skeletal muscle of non-obese men (2015)
  • Author(s): 3.Kakehi S, Tamura Y, Takeno K, Sakurai Y, Kawaguchi M, Watanabe T, Funayama T, Sato F, Ikeda S, Kanazawa A, Fujitani Y, Kawamori R, Watada H
  • Organizer: 2nd Congress, International Academy of Sportology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2015-09-12
  • Int'l Joint Research
• [Presentation] Physical inactivity and high fat diet synergistically enhance the accumulation of intramyocellular diacylglycerol and induce insulin resistance in murine soleus muscle (2015)
  • Author(s): 2.Kakehi S, Tamura Y, Kawamori R, Watada H
  • Organizer: Cell Symposia: Exercise Metabolism
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Presentation] Mechanisums of insulin resistance after 24hours physical inactivity in murine soleus muscle (2015)
  • Author(s): Kakehi S, Tamura Y, Ikeda S, Kawamori R, Watada H
  • Organizer: 62nd Annual Meeting of American College of Sports Medicine
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-05-26
  • Int'l Joint Research
• [Presentation] 高脂肪食負荷による骨格筋細胞内脂質蓄積は骨格筋中の脂質代謝関連遺伝子発現と関連する (2014)
  • Author(s): 筧 佐織、田村 好史、池田 真一、河盛 隆造、綿田 裕孝
  • Organizer: 第35回 日本肥満学会
  • Place of Presentation: 宮崎
  • Year and Date: 2014-10-24 – 2014-10-25
• [Presentation] Accumulation of intramyocellular lipid after high-fat diet is associated with the expression of genes involved in lipid metabolism in non-obese men (2014)
  • Author(s): Kakehi S, Tamura Y, TakenoK, Sakurai Y, Kawaguchi M, Watanabe T, Funayama T, Sato F, Ikeda S, Kanazawa A, Fujitani Y, Kawamori R, and Watada H
  • Organizer: 3rd international conference on Recent Advances and Controversies in Measuring Energy Metabolism
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2014-10-10 – 2014-10-12
• [Presentation] Physical inactivity and high fat diet synergistically enhance the accumulation of intramyocellular diacylglycerol and induce insulin resistance in murine soleus muscle. (2014)
  • Author(s): Kakehi S, Tamura Y, Ikeda S, Kawamori R,Watada H
  • Organizer: 50th EASD Annual Meeting
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2014-09-15 – 2014-09-19
• [Presentation] 不活動による骨格筋インスリン抵抗性惹起における脂質代謝酵素活性変化を介した骨格筋細胞内脂質の関与 (2014)
  • Author(s): 筧 佐織、田村 好史、池田 真一、河盛 隆造、綿田 裕孝
  • Organizer: 第57回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-22 – 2014-05-24
• [Presentation] スプリンターの下肢筋力および体幹筋部と100m走における加速能力との関係 (2013)
  • Author(s): 藤田真平、桜庭景植、佐久間和彦、柳谷登志雄、窪田敦之
  • Organizer: 第68回日本体力医学会
  • Place of Presentation: 東京
• [Presentation] 高脂肪食・不活動による骨格筋インスリン抵抗性発生メカニズム (2013)
  • Author(s): 筧佐織、田村好史、池田真一、河盛隆造、綿田裕孝
  • Organizer: 第56回日本糖尿病学会年次学術集会
  • Place of Presentation: 熊本
• [Presentation] Physical inactivity and high fat diet synergistically enhance the accumulation of intramyocellular diacylglycerol and induce insulin resistance in murine soleus muscle. (2013)
  • Author(s): Kakehi S, Tamura Y, Ikeda S, Kawamori R,Watada H
  • Organizer: American Diabetes Association 73st scientific sessions
  • Place of Presentation: Chicago
• [Presentation] Physical inactivity and high fat diet synergistically enhance the accumulation of intramyocellular diacylglycerol and induce insulin resistance in murine soleus muscle. (2013)
  • Author(s): Kakehi S, Tamura Y, Ikeda S, Kawamori R,Watada H
  • Organizer: 18th Annual Congress of the European college of sports science
  • Place of Presentation: Barcelona
• [Presentation] 高脂肪食・不活動による骨格筋インスリン抵抗性発生メカニズム (2013)
  • Author(s): 筧佐織、田村好史、池田真一、河盛隆造、綿田裕孝
  • Organizer: 第68回日本体力医学会大会
  • Place of Presentation: 東京
• [Presentation] 高脂肪食・不活動による骨格筋インスリン抵抗性発生メカニズム (2013)
  • Author(s): 筧佐織、田村好史、池田真一、河盛隆造、綿田裕孝
  • Organizer: 第34回日本肥満学会/APDO symposium 2013
  • Place of Presentation: 東京
• [Presentation] 膝関節に対する運動療法・筋力トレーニングの up to date ―血流制限下における筋萎縮予防、および筋力訓練効果を中心に―
  • Author(s): 桜庭景植
  • Organizer: 日本整形外科学会（教育研修講演）
  • Place of Presentation: 広島
  • Invited
• [Presentation] 膝関節に対する運動療法・筋力トレーニングの up to date ―血流制限下における筋萎縮予防、および筋力訓練効果を中心に―
  • Author(s): 桜庭景植
  • Organizer: 運動器疾患 / 骨・関節フォーラム
  • Place of Presentation: 東京
  • Invited