Development of a hybrid microscope to observe inner structures and their variation of living biological cells in situ.
| Project/Area Number |
25390134
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Quantum beam science
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
KADO Masataka 国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究主幹 (30360431)
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| Co-Investigator(Renkei-kenkyūsha) |
KISHIMOTO Maki 国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究主幹 (40360432)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 軟X線顕微鏡 / 相関顕微法 / 細胞内小器官 / アポトーシス / 生きている細胞 / レーザープラズマ軟X線顕微鏡 / 蛍光顕微鏡 / ハイブリッド顕微鏡 / 生きている細胞の観察 / ミトコンドリア |
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| Outline of Final Research Achievements |
I have developed a hybrid microscopy which is a combination of a fluorescent microscope and a soft X-ray microscope and this microscopy enabled to observe live biological cells with both microscopes at the same time. Observing Leydig cells of mouse testis with the hybrid microscopy, I have succeeded to obtain a soft X-ray image of a single mitochondrion in a live biological cell. The hybrid microscopy also was applied to observe apoptotic cells and details of structural deformation due to the apoptosis have been cleared.
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Report
(4 results)
Research Products
(22 results)
