| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Cationic polymer - amphiphilic anion complexes can easily cross the lipid bilayer membrane, which can be detected as fluorescence emission by using fluorogenic liposome. Presence of competitive anions that hinder the formation of polymer-counteranion complexes should reduce the translocation activity. Consumption of the competitive anions or production of activator anions during enzyme reactions led into fluorogenic response. Activities of enzymes, such as kinases and sulfotransferases, were readily evaluated by the “naked eye” detection system. The simple mix-and-measure sensing method will provide a cost-effective enzyme assay kit.
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