Enzyme assay system by using a fluorogenic liposome
| Project/Area Number |
25410154
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Ryukoku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 膜透過性ペプチド / 酵素活性評価技術 / リポソーム / 蛍光センシング / 酵素 / 酵素阻害剤 |
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| Outline of Final Research Achievements |
Cationic polymer - amphiphilic anion complexes can easily cross the lipid bilayer membrane, which can be detected as fluorescence emission by using fluorogenic liposome. Presence of competitive anions that hinder the formation of polymer-counteranion complexes should reduce the translocation activity. Consumption of the competitive anions or production of activator anions during enzyme reactions led into fluorogenic response. Activities of enzymes, such as kinases and sulfotransferases, were readily evaluated by the “naked eye” detection system. The simple mix-and-measure sensing method will provide a cost-effective enzyme assay kit.
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Report
(4 results)
Research Products
(10 results)
