G-quadruplex formation controlled by irradiation of triphenylmethane derivatives

• Project/Area Number: 25410185
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Nara National College of Technology
• Principal Investigator: Uda Ryoko 奈良工業高等専門学校, その他部局等, 准教授 (90321463)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 光応答 / グアニン四重鎖 / トリフェニルメタン

Outline of Final Research Achievements

Photoresponsive malachite green copolymer has been designed to induce the formation of G-quadruplex by light. The interaction of the copolymer with d[(TTAGGG)4] oligonucleotide has been investigated by fluorescence analysis. Circular dichroism spectroscopies revealed that irradiation of the mixture of the copolymer and d[(TTAGGG)4] leads to the formation of antiparallel G-quadruplex. The results of competition dialysis assay indicated that the binding of malachite green to G-quadruplex of d[(TTAGGG)4] was selective. The G-quadruplex structure was found to be stabilized by the binding of malachite green.

Research Products

• [Journal Article] Photoinduced conformational changes in DNA by poly(vinyl alcohol) carrying a malachite green moiety for protecting DNA against attack by nuclease (2015)
  • Author(s): Uda, R. M. Matsui, T.
  • Journal Title: Soft Matter Volume: 11 Issue: 42 Pages: 8246-8252
  • DOI: 10.1039/c5sm01874a
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] マラカイトグリーンコポリマーの光イオン化反応がもたらすグアニン四重鎖形成の促進 (2016)
  • Author(s): 松井 誉・宇田亮子
  • Organizer: 日本化学会 第96春季年会
  • Place of Presentation: 同志社大学
  • Year and Date: 2016-03-24
• [Presentation] マラカイトグリーン含有光応答性コポリマーとオリゴヌクレオチドとの相互作用 (2015)
  • Author(s): 松井 誉・宇田亮子
  • Organizer: 2015年光化学討論会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2015-09-11
• [Presentation] マラカイトグリーンコポリマーへの光照射によるグアニン四重鎖形成 (2015)
  • Author(s): 西本徳子、宇田亮子
  • Organizer: 日本化学会第９５春季年会
  • Place of Presentation: 日本大学 理学部・薬学部
  • Year and Date: 2015-03-28
• [Presentation] 光応答性マラカイトグリーンコポリマーがもたらすDNase耐性 (2015)
  • Author(s): 松井誉始、宇田亮子
  • Organizer: 日本化学会第９５春季年会
  • Place of Presentation: 日本大学 理学部・薬学部
  • Year and Date: 2015-03-27
• [Presentation] 光イオン化マラカイトグリーン誘導体とオリゴヌクレオチドの錯形成 (2014)
  • Author(s): 宇田亮子
  • Organizer: 第60回高分子研究発表会（神戸）
  • Place of Presentation: 兵庫県民会館
  • Year and Date: 2014-07-24 – 2014-07-25
• [Presentation] 光イオン化マラカイトグリーン誘導体との錯体形成に伴うDNAの可溶化 (2014)
  • Author(s): 宇田亮子
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学
• [Presentation] 光イオン化マラカイトグリーン誘導体とオリゴヌクレオチドの錯形成 (2014)
  • Author(s): 宇田亮子
  • Organizer: 第60回高分子研究発表会
  • Place of Presentation: 兵庫県民会館