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The Role of Anchor in the Prod1 essential in Newt Limb Regeneration

Research Project

Project/Area Number 25410187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionSuntory Foundation for Life Sciences

Principal Investigator

Miura Kaoru  公益財団法人サントリー生命科学財団, その他部局等, 研究員 (90353515)

Co-Investigator(Renkei-kenkyūsha) SHIMANOTO Keiko  公益財団法人サントリー生命科学財団, 生物有機科学研究所, 研究員 (70235638)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsGPIアンカー型タンパク質 / 固体NMR / 肢再生 / 脂質二重膜 / 固体NMR / 肢の再生 / GPIアンカー型蛋白質
Outline of Final Research Achievements

Newt Prod1 is tethered to the cell membrane by a glycosylphosphatidylinositol(GPI) anchor. We clarified the role for the anchoring of Prod1 using synthetic mimic of GPI-anchored Prod1 by solid-state NMR, fluorescence microscopy, and DLS measurement. Anchoring to the membrane drastically reduced the motion of Prod1 which attributed to the aggregation formation of anchored Prod1 on membranes. Three dimensional structure of the anchored Prod1 was not affected by aggregates formation on the membranes. The aggregated anchored Prod1 easily interacted with the anchored Prod1 tethered on opposing membrane surfaces, inducing membrane adhesion. Anchoring to the membrane is one of the reasons of the aggregation and following cell adhesion, but the high affinity between Prod1 should be also required for these phenomena. Thus, our results strongly assert the role of anchor in the salamander-specific protein Prod1 in assisting the cell adhesion function in the limb regeneration process.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Book (1 results)

  • [Journal Article] Solid-State NMR Studies of Antimicrobial Peptides from Arachnid Venoms2014

    • Author(s)
      Nomura K, Villegas E, Corzo G
    • Journal Title

      Biomedical Spectroscopy and Imaging

      Volume: 3 Issue: 2 Pages: 107-120

    • DOI

      10.3233/bsi-140082

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Solid-state NMR spectra of lipid-anchored proteins under magic angle spinning.2014

    • Author(s)
      Nomura, K., Harada, E., Sugase, K., Shimamoto, K.
    • Journal Title

      J. Phys. Chem. B.

      Volume: 118 Issue: 9 Pages: 2405-2413

    • DOI

      10.1021/jp4124106

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Solid-state NMR and Microscopic Studies of Synthetic Mimic of GPI-anchored Proteins2015

    • Author(s)
      Kaoru Nomura, Erisa Harada, Kenji Sugase, Keiko Shimamoto, Fumio Hayashi, and Kenichi Morigaki
    • Organizer
      2015環太平洋国際化学会議(Pacifichem 2015)
    • Place of Presentation
      ホノルル(ハワイ)
    • Year and Date
      2015-12-17 – 2015-12-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Solid state NMR studies of crystalline state DMPC and a synthetic mimic of GPI-anchored protein in membranes2015

    • Author(s)
      Kaoru Nomura
    • Organizer
      2015環太平洋国際化学会議(Pacifichem 2015)
    • Place of Presentation
      ホノルル(ハワイ)
    • Year and Date
      2015-12-15 – 2015-12-16
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Solid state NMR studies of crystalline state DMPC and a synthetic mimic of GPI-anchored protein in membranes2015

    • Author(s)
      Kaoru Nomura
    • Organizer
      Pacifichem2015
    • Place of Presentation
      ホノルル
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Solid-state NMR and Microscopic Studies of Synthetic Mimic of GPI-anchored Proteins2015

    • Author(s)
      Kaoru Nomura
    • Organizer
      Pacifichem2015
    • Place of Presentation
      ホノルル
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脂質二重膜とそれに繋がれたタンパク質の固体NMR2015

    • Author(s)
      野村薫
    • Organizer
      第58回固体NMR・材料フォーラム
    • Place of Presentation
      高知大学
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] GPIアンカー型蛋白質模倣分子の脂質二重膜存在下での固体NMR測定2013

    • Author(s)
      野村、原田、菅瀬、島本
    • Organizer
      日本膜学会第35年会 5.20-5.21 (東京)
    • Place of Presentation
      東京(早稲田大学西早稲田キャンパス)
    • Related Report
      2013 Research-status Report
  • [Presentation] Solid-state NMR Spectra of Pseudo-GPI-anchored Proteins in Membranes under Magic Angle2013

    • Author(s)
      Nomura, Harada, Sugase, Shimamoto
    • Organizer
      第5回Asia-Pacific NMR Symposium
    • Place of Presentation
      ブリスベン(Brisbane Convention and Exhibition Centre)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] GPIアンカー型蛋白質模倣分子の脂質二重膜存在下での固体NMR測定2013

    • Author(s)
      野村、原田、菅瀬、島本
    • Organizer
      第52回NMR討論会
    • Place of Presentation
      金沢(石川県立音楽堂)
    • Related Report
      2013 Research-status Report
  • [Presentation] Solid-state NMR Spectra of Pseudo GPI-anchored Proteins in membranes under Magic Angle Spinning

    • Author(s)
      野村
    • Organizer
      ERATOタンパク情報交換会
    • Place of Presentation
      大阪(大阪大学)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] Advances in Biological Solid-State NMR: Proteins and Membrane-Active Peptides2014

    • Author(s)
      Kaoru Nomura and Shoichi Kusumoto (Chapter 9 Lipopolysaccharide Induces Raft Domain Expansion in a Cholesterol-Containing Membrane)
    • Publisher
      RSC Publishing
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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