Development of new biological adhesive based on chitin derivatives and chitin nanofibers

• Project/Area Number: 25410227
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Polymer/Textile materials
• Research Institution: Tottori University
• Principal Investigator: SAIMOTO HIROYUKI 鳥取大学, 工学(系)研究科(研究院), 教授 (20186977)
• Co-Investigator(Renkei-kenkyūsha): OKAMOTO Yoshiharu 鳥取大学, 農学部, 教授 (50194410) ; MORIMOTO Minoru 鳥取大学, 生命機能研究支援センター, 准教授 (10273880) ; IFUKU Shinsuke 鳥取大学, 大学院工学研究科, 准教授 (70402980) ; OSAKI Tomohiro 鳥取大学, 農学部, 准教授 (40431332)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: キチン / キトサン / 医用接着剤 / シーリング材料 / 生体適合性 / 医用材料 / 生体接着剤 / ナノファイバー

Outline of Final Research Achievements

Novel composite materials were made from chitin methacrylate derivatives and various chitin nanofibers. The adhesive strength of the chitin methacrylate derivatives was much improved when they were blended with chitin nanofibers or surface-deacetylated chitin nanofibers. Moreover, these composite materials showed good biocompatibility. These findings indicate that the composites prepared in this study are promising materials as new biological adhesives.

Research Products

• [Journal Article] Biological adhesive based on carboxymethyl chitin derivatives and chitin nanofibers (2015)
  • Author(s): Kazuo Azuma, Masahiro Nishihara, Haruki Shimizu, Yoshiki Ito, Osamu Takashima, Tomohiro Osaki, Norihiko Ito, Tomohiro Imagawa, Yusuke Murahata, Takeshi Tsuka, Hironori Izawa, Shinsuke Ifuku, Saburo Minami, Hiroyuki Saimoto, Yoshiharu Okamoto, Minoru Morimoto
  • Journal Title: Biomaterials Volume: 42 Pages: 20-29
  • DOI: 10.1016/j.biomaterials.2014.11.043
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Green Approach to Endow Chitin Nanofiber with Fluorescence (2015)
  • Author(s): Ajoy Kumar Dutta, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto, Shinsuke Ifuku
  • Journal Title: Journal of Chitin and Chitosan Science Volume: 3 Issue: 1 Pages: 53-56
  • DOI: 10.1166/jcc.2015.1085
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Chitin, Chitosan, and Its Derivatives for Wound Healing: Old and New Materials (2015)
  • Author(s): Kazuo Azuma, Ryotaro Izumi, Tomohiro Osaki, Shinsuke Ifuku, Minoru Morimoto, Hiroyuki Saimoto, Saburo Minami, Yoshiharu Okamoto
  • Journal Title: Journal Functional Biomaterials Volume: 6 Issue: 1 Pages: 104-142
  • DOI: 10.3390/jfb6010104
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Preparation of zwitterionically charged nanocrystals by surface TEMPO-mediated oxidation and partial deacetylation of alpha-chitin (2015)
  • Author(s): S. Ifuku, T. Hori, H. Izawa, M. Morimoto, H. Saimoto
  • Journal Title: Carbohydrate Polymers Volume: 122 Pages: 1-4
  • DOI: 10.1016/j.carbpol.2014.12.060
  • Peer Reviewed / Open Access
• [Journal Article] Effects of surface-deacetylated chitin nanofibers in an experimental model of hypercholesterolemia (2015)
  • Author(s): K. Azuma, T. Nagae, T. Nagai, H. Izawa, M. Morimoto, Y. Murahata, T. Osaki, T. Tsuka, T. Imagawa, N. Ito, Y. Okamoto, H. Saimoto, S. Ifuku
  • Journal Title: International Journal of Molecular Sciences Volume: 16 Issue: 8 Pages: 17445-17455
  • DOI: 10.3390/ijms160817445
  • Peer Reviewed / Open Access
• [Journal Article] Characterizaion of chitosan nanofiber Sheets for Antifungal Application (2015)
  • Author(s): Mayumi Egusa, Ryo Iwamoto, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto, Hironori Kaminaka, Shinsuke Ifuku
  • Journal Title: International Journal of Molecular Sciences Volume: 16 Pages: 26202-26210
  • Peer Reviewed / Open Access
• [Journal Article] Protective effect of chitin urocanate nanofibers against ultraviolet radiation (2015)
  • Author(s): Ikuko Ito, Toshikazu Yoneda, Yoshihiko Omura, Tomohiro Osaki*, Shinsuke Ifuku, Hiroyuki Saimoto, Kazuo Azuma, Tomohiro Imagawa, Takeshi Tsuka, Yusuke Murahata, Norihiko Ito, Yoshiharu Okamoto, Saburo Minami
  • Journal Title: Marine Drugs Volume: 13 Issue: 12 Pages: 7463-7475
  • DOI: 10.3390/md13127076
  • Peer Reviewed / Open Access
• [Journal Article] Preparation of a protein-chitin nanofiber complex from crab shells and its application as a reinforcement filler or substrate for biomineralization (2015)
  • Author(s): S. Ifuku, T. Urakami, H. Izawa, M. Morimoto, H. Saimoto
  • Journal Title: RSC Advances Volume: 5 Issue: 79 Pages: 64196-64201
  • DOI: 10.1039/c5ra12761k
  • Peer Reviewed
• [Journal Article] Facile preparation of silver nanoparticles immobilized on chitin nanofiber surfaces to endow antifungal activities (2015)
  • Author(s): S. Ifuku, Y. Tsukiyama, T. Yukawa, M. Egusa, H. Kaminaka, H. Izawa, M. Morimoto, H. Saimoto
  • Journal Title: Carbohydrate Polymers Volume: 117 Pages: 813-817
  • DOI: 10.1016/j.carbpol.2014.10.042
  • Peer Reviewed
• [Journal Article] Preparation of chitosan nanofibers from completely deacetylated chitosan powder by a downsizing process (2015)
  • Author(s): Yihun Fantahun Akilog, Ajoy Kumar Dutta, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto, Shinsuke Ifuku
  • Journal Title: International Journal Biological Macromolecules Volume: 72 Pages: 1191-1195
  • DOI: 10.1016/j.ijbiomac.2014.10.042
  • Peer Reviewed
• [Journal Article] Facile preparation of surface N-halamine chitin nanofiber to endow antibacterial and antifungal activities (2015)
  • Author(s): Ajoy Kumar Dutta, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto, Shinsuke Ifuku
  • Journal Title: Carbohydrate Polymers Volume: 115 Pages: 342-347
  • DOI: 10.1016/j.carbpol.2014.08.094
  • Peer Reviewed
• [Journal Article] Synthesis and functionality of chitin and chitosan derivatives (2014)
  • Author(s): M. Morimoto, H. Izawa, S. Ifuku, H. Saimoto
  • Journal Title: Chitin and Chitosan Res. Volume: 20 Pages: 204-211
  • NAID: 40020252142
  • Peer Reviewed
• [Journal Article] Surface maleylation and naphthaloylation of chitin nanofibers for property enhancement (2014)
  • Author(s): S. Ifuku, N. Suzuki, H. Izawa, M. Morimoto, H. Saimoto
  • Journal Title: Reactive & Functional Polymers Volume: 85 Pages: 121-125
  • DOI: 10.1016/j.reactfunctpolym.2014.06.008
  • Peer Reviewed
• [Journal Article] Novel preparation of chitin nanocrystals by H2SO4 and H3PO4 hydrolysis followed by high-pressure water jet treatments (2014)
  • Author(s): A. K. Dutta, H. Izawa, M. Morimoto, H. Saimoto, S. Ifuku
  • Journal Title: Journal of Chitin and Chitosan Science Volume: 2 Issue: 3 Pages: 179-184
  • DOI: 10.1166/jcc.2014.1067
  • Peer Reviewed
• [Journal Article] Facile preparation of aramid nanofibers from Twaron fibers by a downsizing process (2014)
  • Author(s): S. Ifuku, H. Maeta, H. Izawa, M. Morimoto, H. Saimoto
  • Journal Title: RSC Advances Volume: 4 Issue: 76 Pages: 40377-40380
  • DOI: 10.1039/c4ra06924b
  • Peer Reviewed
• [Journal Article] Effect of chitin nanofibril combined in rayon animal bedding on hairless mouse skin and on a three dimensional culture human skin model (2014)
  • Author(s): I. Ito, T. Osaki, K. Tokuda, T. Asami, Y. Takamori, S. Kurozum, S. Ifuku, H. Saimoto, T. Imagawa, K. Azuma, T. Tsuka, Y. Okamoto, S. Minami
  • Journal Title: Journal of Chitin and Chitosan Science Volume: 2 Issue: 2 Pages: 82-88
  • DOI: 10.1166/jcc.2014.1040
  • Peer Reviewed
• [Journal Article] Surface phthaloylation of chitin nanofiber in aqueous media to improve solubility in aromatic solvents and give thermo-responsive and ultraviolet protection protperties (2014)
  • Author(s): S. Ifuku, N. Suzuki, H. Izawa, M. Morimoto, H. Saimoto
  • Journal Title: RSC Advances Volume: 4 Issue: 37 Pages: 19246-19250
  • DOI: 10.1039/c4ra01975j
  • Peer Reviewed
• [Journal Article] Depolymerization of sulfated polysaccharides under hydrothermal conditions (2014)
  • Author(s): Minoru Morimoto, Masaaki Takatori, Tetsuya Hayashi, Daiki Mori, Osamu Takashima, Shinichi Yoshida, Kimihiko Sato, Hitoshi Kawamoto, Jun-ichi Tamura, Hironori Izawa, Shinsuke Ifuku, Hiroyuki Saimoto
  • Journal Title: Carbohydrate Research Volume: 384 Pages: 56-60
  • DOI: 10.1016/j.carres.2013.11.017
  • Peer Reviewed
• [Journal Article] Evaluation of the effect of chitin nanofibris on skin functions using skin models (2014)
  • Author(s): Ikuko Ito, Tomohiro Osaki, Shinsuke Ifuku, Hiroyuki Saimoto, Yoshimori Takamori, Seiji Kurozumi, Tomohiro Imagawa, Kazuo Azuma, Takeshi Tsuka, Yoshiharu Okamoto, Saburo Minami
  • Journal Title: Carbohydrate Polymers Volume: 101 Pages: 464-470
  • DOI: 10.1016/j.carbpol.2013.09.074
  • Peer Reviewed
• [Journal Article] Control of mechanical properties of chitin nanofiber film using glycerol without losing its characteristics (2014)
  • Author(s): Shinsuke Ifuku, Akiko Ikuta, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto
  • Journal Title: Carbohydrate Polymers Volume: 101 Pages: 714-717
  • DOI: 10.1016/j.carbpol.2013.09.076
  • Peer Reviewed
• [Journal Article] Anti-inflammatory effects of cellulose nanofiber made from pear in inflammatory bowel disease model (2014)
  • Author(s): Kazuo Azuma, Tomohiro Osaki, Shinsuke Ifuku, Hiroyuki Saimoto, Osamu Takashima, Takeshi Tsuka, Tomohiro Imagawa, Yoshiharu Okamoto, Saburo Minami
  • Journal Title: Bioactive Carbohydrates and Dietary Fibre Volume: 3 Issue: 1 Pages: 1-10
  • DOI: 10.1016/j.bcdf.2013.11.001
  • Peer Reviewed
• [Journal Article] Fiber width and molecular weight studies of alpha-chitin nanofibers (2013)
  • Author(s): Ajoy Kumar Dutta, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto, Shinsuke Ifuku
  • Journal Title: Jouranl of Chitin and Chitosan Science Volume: 1 Issue: 3 Pages: 192-196
  • DOI: 10.1166/jcc.2013.1024
  • Peer Reviewed
• [Journal Article] Simple Preparation of Chitin Nanofibers from Dry Squid Pen β-chitin Powder by the Star Burst System (2013)
  • Author(s): Ajoy Kumar Dutta, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto, Shinsuke Ifuku
  • Journal Title: Jouranl of Chitin and Chitosan Science Volume: 1 Issue: 3 Pages: 186-191
  • DOI: 10.1166/jcc.2013.1023
  • Peer Reviewed
• [Journal Article] Preparation of high-strength and transparent chitosan film reinforced with surface deacetylated chitin nanofibers (2013)
  • Author(s): Ifuku, S., Ikuta, A., Egusa, M., Kaminaka, H., Izawa, H., Morimoto, M., Saimoto, H.
  • Journal Title: Carbohydrate Polymymers Volume: 98 Issue: 1 Pages: 1198-1202
  • DOI: 10.1016/j.carbpol.2013.07.033
  • Peer Reviewed
• [Journal Article] Simple preparation of chitosan nanofibers from dry chitosan powder by the Star Burst system (2013)
  • Author(s): Ajoy Kumar Dutta, Naoki Kawamoto, Gaku Sugino, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto, Shinsuke Ifuku
  • Journal Title: Carbohydrate Polymers Volume: 97 Issue: 2 Pages: 363-367
  • DOI: 10.1016/j.carbpol.2013.05.010
  • Peer Reviewed
• [Journal Article] A Comparative Study Analysis of alpha-Chitin and beta-Chitin Nanofibrils by Using an Inflammatory-Bowel Disease Mouse Model (2013)
  • Author(s): Kazuo Azuma, Tomohiro Osaki, Shinsuke Ifuku, Hiroyuki Saimoto, Takeshi Tsuka, Tomohiro Imagawa, Yoshiharu Okamoto, and Saburo Minami
  • Journal Title: Journal of Chitin and Chitosan Science Volume: 1 Issue: 2 Pages: 144-149
  • DOI: 10.1166/jcc.2013.1020
  • Peer Reviewed
• [Presentation] 表面脱アセチル化キチンナノファイバーの創傷治癒促進効果 (2015)
  • Author(s): 泉良太郎, 東和生, 井澤浩則, 森本稔, 斎本博之, 伊福伸介
  • Organizer: 第29回日本キチン・キトサン学会大会
  • Place of Presentation: 東海大学熊本キャンパス
  • Year and Date: 2015-08-21
• [Presentation] N-硫酸化シクロデキストリングラフトキトサンの調製 (2015)
  • Author(s): 吉橋雅, 伊福伸介, 森本稔, 斎本博之, 井澤浩則
  • Organizer: 第29回日本キチン・キトサン学会大会
  • Place of Presentation: 東海大学熊本キャンパス
  • Year and Date: 2015-08-20
• [Presentation] 完全脱アセチル化キトサンからの粉砕によるナノファイバーの調製 (2015)
  • Author(s): Yihun F. Akilog, Ajoy K. Dutta, 井澤浩則, 森本稔, 斎本博之, 伊福伸介
  • Organizer: 第29回日本キチン・キトサン学会大会
  • Place of Presentation: 東海大学熊本キャンパス
  • Year and Date: 2015-08-20
• [Presentation] 表面脱アセチル化キチンナノファイバーの抗肥満効果 (2014)
  • Author(s): 東和生、大崎智弘、今川智敬、柄武志、伊藤典彦、村端悠介、井澤浩則、斎本博之、南三郎、岡本芳晴、伊福伸介
  • Organizer: 第28回キチン・キトサンシンポジウム
  • Place of Presentation: 順天堂大学
  • Year and Date: 2014-08-07 – 2014-08-08
• [Presentation] Fascile N-chlorination of chitin nanofibers for endowing anti-hungal and bacterial properties (2014)
  • Author(s): A. K. Dutta, 江草真由美、井澤浩則、森本 稔、斎本博之、伊福伸介
  • Organizer: 第28回キチン・キトサンシンポジウム
  • Place of Presentation: 順天堂大学
  • Year and Date: 2014-08-07 – 2014-08-08
• [Presentation] 生物材料由来の新規細胞培養基質 (2014)
  • Author(s): 小川陽子、斎本博之、井澤浩則、森本 稔、伊藤典彦、伊福伸介
  • Organizer: 第28回キチン・キトサンシンポジウム
  • Place of Presentation: 順天堂大学
  • Year and Date: 2014-08-07 – 2014-08-08
• [Presentation] Development of tissue adhesive using carboxymethyl chitin derivatives (2013)
  • Author(s): Yoshiki Ito, Haruki Shimizu, Masahiro Nishihar, Osamu Takashima, Hironori Izawa, Shinsuke Ifuku, Minoru Morimoto, Tomohiro Osaki, Yoshiharu Okamoto, Saburo Minami, Hiroyuki Saimoto
  • Organizer: 10th Asia-Pacific Chitin & Chitosan Symposium
  • Place of Presentation: Yonago Convention Center, Japan
• [Presentation] Development of biological adhesive agents by using chitin nanofibers (2013)
  • Author(s): Masahiro Nishihara, Tomohiro Osaki, Yoshiki Itoh, Haruki Shimizu, Osamu Takashima, Hironori Izawa, Minoru Morimoto, Shinsuke Ifuku, Hiroyuki Saimoto, Kazuo Azuma, Tomohiro Imagawa, Takeshi Tsuka, Yoshiharu Okamoto, Saburo Minami
  • Organizer: 10th Asia-Pacific Chitin & Chitosan Symposium
  • Place of Presentation: Yonago Convention Center, Japan
• [Presentation] Preparation of high-strength transparent film reinforced with surface-deacetylated chitin nanofibers (2013)
  • Author(s): Shinsuke Ifuku, Akiko Ikuta, Mayumi Egusa, Hironori Kaminaka, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto
  • Organizer: 10th Asia-Pacific Chitin & Chitosan Symposium
  • Place of Presentation: Yonago Convention Center, Japan
• [Presentation] Preparation of chitin and chitosan nanofibers from dry chitin and chitosan powder by using star burst system (2013)
  • Author(s): Ajoy Dutta, Hironori Izawa, Minoru Morimoto, Hiroyuki Saimoto, Shinsuke Ifuku
  • Organizer: 10th Asia-Pacific Chitin & Chitosan Symposium
  • Place of Presentation: Yonago Convention Center, Japan
• [Presentation] Method for replacing water in chitin nanofibers suspension with organic solvent (2013)
  • Author(s): Toshikazu Yoneda, Shinsuke Ifuku, Hiroyuki Saimoto, Yoshihiko Omura
  • Organizer: 10th Asia-Pacific Chitin & Chitosan Symposium
  • Place of Presentation: Yonago Convention Center, Japan
• [Presentation] Preparation of N-phthaloylated chitin nanofibers and its characterization (2013)
  • Author(s): Nana Suzuki, Akihiro Matsui, Shinsuke Ifuku, Minoru Morimoto, Hiroyuki Saimoto
  • Organizer: 10th Asia-Pacific Chitin & Chitosan Symposium
  • Place of Presentation: Yonago Convention Center, Japan
• [Presentation] A comparative study between α-chitin nanofibrils and β-chitin nanofibrils using an inflammatory bowel disease mouse model (2013)
  • Author(s): Kazuo Azuma, Tomohiro Osaki, Tomohiro Imagawa, Takeshi Tsuka, Shinsuke Ifuku, Hiroyuki Saimoto, Yoshiharu Okamoto, Saburo Minami
  • Organizer: 10th Asia-Pacific Chitin & Chitosan Symposium
  • Place of Presentation: Yonago Convention Center, Japan