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Studies on plasticity and brittleness of protein crystals by using synchrotron digital X-ray topography

Research Project

Project/Area Number 25420694
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical properties of metals/Metal-base materials
Research InstitutionYokohama Soei University

Principal Investigator

KOJIMA Kenichi  横浜創英大学, こども教育学部, 教授 (90046095)

Co-Investigator(Kenkyū-buntansha) WAKO Kei  横浜創英大学, こども教育学部, 講師 (40515839)
TACHIBANA Masaru  横浜市立大学, 大学院・生命ナノシステム科学研究科, 教授 (80236546)
KOIZUMI Haruhiko  東北大学, 金属材料研究所, 助教 (10451626)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywordsタンパク質結晶 / 力学的性質 / 結晶塑性 / 脆性 / 放射光X線トポグラフ / 材料強度 / デジタル画像 / 転位 / 格子欠陥 / 結晶の完全性 / 力学特性 / 塑性 / 弾性 / 結晶の強度 / 放射光トポグラフ / タンパク質結晶の弾性 / タンパク質結晶の塑性 / X線トポグラフ / 結晶の脆性 / 結晶の弾性
Outline of Final Research Achievements

Proteins are one of three main organic nutrients consisting of carbohydrates and fats and components in food that an organism uses to survive and grow. These proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues. For example, the hen-egg white lysozyme is shown as one of typical proteins. These kinds of protein molecules could be crystalized as same as a table salt. In this research, we carried out fundamental experiments on mechanical properties of protein crystals such as hen-egg white lysozyme and glucose isomerase crystals. We found some important results as follows; ①the plastic deformation of protein crystals are controlled by the normal dislocation mechanisms. ②the strength of protein crystals could be improved by chemical binding and gel-method.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (24 results)

All 2016 2015 2014 2013

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 5 results,  Acknowledgement Compliant: 6 results) Presentation (14 results) Book (1 results)

  • [Journal Article] タンパク質結晶の放射光X線トポグラフィ2016

    • Author(s)
      橘 勝、鈴木 凌、小泉 晴比古、若生 啓、小島 謙一
    • Journal Title

      結晶成長学会誌

      Volume: 未定

    • NAID

      130005997169

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Hardness and slip systems of orthorhombic hen egg-white lysozyme crystals2016

    • Author(s)
      Ryo Suzuki, Takeharu Kishi, Shiro Tsukashima, Masaru Tachibana, Kei Wako and Kenichi Kojima
    • Journal Title

      Philosophical Magazine

      Volume: 未定

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Crystallization of High-Quality Protein Crystals Using an External Electric Field2015

    • Author(s)
      Koizumi, Haruhiko; Uda, Satoshi; Fujiwara, Kozo; Tachibana, Masaru; Kojima, Kenichi; Nozawa,
    • Journal Title

      J. Appl. Crystallgr.

      Volume: 48 Pages: 1507-1513

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Dislocations in High-quality Glucose Isomerase Crystals Grown from Seed Crystals2014

    • Author(s)
      H. Koizumi, M. Tachibana, I Yoshizaki, S. Fukuyama, K. Tsukamoto, Y. Suzuki, S. Ueda and K. Kojima
    • Journal Title

      J. Cryst. Growth and Desighn

      Volume: 14 Pages: 5111-5116

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Crystal distortion of monoclinic hen egg-white lysozyme crystals using X-ray digital topography2014

    • Author(s)
      Kei Wako, Daiki Fujii, Shiro Tsukashima,Takeharu Kishi, Masaru Tachibana and Kenichi Kojima
    • Journal Title

      J Cryst. Grwth

      Volume: 401 Pages: 238-241

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Elastic constants in orthorhombic hen egg-white lysozyme crystals2014

    • Author(s)
      N. Kitajima, S. Tsukashima, D. Fujii, M. Tachibana, H. Koizumi, K. Wako and K. Kojima
    • Journal Title

      Phys Rev E89 012714- 1- 6 (2014)

      Volume: E89

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Elastic constants in orthorhombic hen egg-white lysozyme crystals,2014

    • Author(s)
      N. Kitajima, S. Tsukashima, D. Fujii, M. Tachibana, H. Koizumi, K. Wako and K. Kojima
    • Journal Title

      Phys. Rev. E

      Volume: 89 Issue: 1 Pages: 127141-127146

    • DOI

      10.1103/physreve.89.012714

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] タンパク質結晶の弾性定数2013

    • Author(s)
      橘 勝、小泉晴比古、小島謙一
    • Journal Title

      日本結晶成長学会誌

      Volume: 40 Pages: 126-134

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Improvement of Crystal Quality for Tetragonal Hen-Egg White Lysozyme2013

    • Author(s)
      Haruhiko Koizumi, Satoshi Uda, Kozo Fujiwara, Masaru Tachibana, Kenichi Kojima and Jun Nozawa
    • Journal Title

      J. Appl. Cryst

      Volume: 46 Pages: 25-29

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] グルコースイソメラーゼ結晶の成長転位の同定2016

    • Author(s)
      鈴木凌、岸健晴、小泉晴比古、塚本勝男、荒井康智、福山誠二郎、鈴木良尚、小島謙一、橘勝
    • Organizer
      日本物理学会 第71回年次大会
    • Place of Presentation
      東北学院大学 泉キャンパス
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] ルコースイソメラーゼ結晶の転位の運動と塑性変形2015

    • Author(s)
      鈴木凌、岸健晴、小泉晴比古、塚本勝男、荒井康智、福山誠二郎、鈴木良尚、小島謙一、橘勝
    • Organizer
      第45回結晶成長国内会議
    • Place of Presentation
      北海道大学 学術交流会館
    • Year and Date
      2015-10-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 放射光単色X線トポグラフィによるグルコースイソメラーゼ結晶中の転位発生の観察2015

    • Author(s)
      鈴木凌、岸健晴、小泉晴比古、塚本勝男、荒井康智、福山誠二郎、鈴木良尚、小島謙一、橘勝
    • Organizer
      日本物理学会 2015年秋季大会
    • Place of Presentation
      関西大学 千里山キャンパス
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] タンパク質結晶の転位の運動と塑性変形2015

    • Author(s)
      鈴木凌、岸健晴、村田秀信、小島謙一、橘勝
    • Organizer
      新学術領域若手の会
    • Place of Presentation
      ホテルグランテラス千歳
    • Year and Date
      2015-07-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] グルコースイソメラーゼ結晶の力学的性質2015

    • Author(s)
      岸健晴、鈴木凌、若生啓、小島謙一、橘勝
    • Organizer
      日本物理学会 第70回年次大会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2015-03-21 – 2015-03-24
    • Related Report
      2014 Research-status Report
  • [Presentation] X線デジタルトポグラフィの解析によるタンパク質結晶の完全性評価2015

    • Author(s)
      若生啓、藤居大毅A、岸健晴A、鈴木凌A、橘勝A、小島謙一
    • Organizer
      日本物理学会 第70回年次大会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2015-03-21 – 2015-03-24
    • Related Report
      2014 Research-status Report
  • [Presentation] ゲル中成長のタンパク質結晶の力学的性質2015

    • Author(s)
      鈴木凌、岸健晴、若生啓、小島謙一、橘勝
    • Organizer
      日本物理学会 第70回年次大会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2015-03-21 – 2015-03-24
    • Related Report
      2014 Research-status Report
  • [Presentation] X線トポグラフィによるグルコースイソメラーゼ結晶の完全性評価2014

    • Author(s)
      岸健晴、鈴木凌、橘勝、小泉晴比古、若生啓、小島謙一、吉崎泉、福山誠二郎、鈴木良尚、塚本勝男
    • Organizer
      第44回結晶成長国内会議
    • Place of Presentation
      学習院大学
    • Year and Date
      2014-11-06 – 2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] X線トポグラフィによるグルコースイソメラーゼ結晶評価2014

    • Author(s)
      岸健晴、小泉晴比古、小島謙一、塚本勝男、鈴木良尚、吉崎泉E、福山誠二郎、橘勝
    • Organizer
      日本物理学会 2014年秋季大会
    • Place of Presentation
      中部大学春日井キャンパス
    • Year and Date
      2014-09-07 – 2014-09-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 鶏卵白リゾチウム結晶の硬さの放置時間依存性2014

    • Author(s)
      岸健晴、塚島史朗、若生啓、小島謙一、橘勝
    • Organizer
      日本物理学会第69回年次大会
    • Place of Presentation
      東海大湘南キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] Enhancement of grystal homogeneity of protein crystals under application of alternative current electric field.2014

    • Author(s)
      H.Koizumi, S.Uda, K.Fujiwara,M.Tachibana, K.Kojima, J.Nozawa
    • Organizer
      10th International Comutational Methods in Sciences and Engineering
    • Place of Presentation
      アテネ(ギリシャ)
    • Related Report
      2013 Research-status Report
  • [Presentation] リゾチウム結晶の塑性2013

    • Author(s)
      塚島史朗、若生啓、小島謙一、橘勝
    • Organizer
      第43回結晶成長学会
    • Place of Presentation
      長野市生涯学習センター
    • Related Report
      2013 Research-status Report
  • [Presentation] 交流電場下での固相と液相のエントロピー操作による高品質タンパク質結晶の育成2013

    • Author(s)
      小泉晴比古、宇田聡、藤原航三、橘勝、小島謙一、野沢純
    • Organizer
      第43回結晶成長学会
    • Place of Presentation
      長野市生涯学習センター
    • Related Report
      2013 Research-status Report
  • [Presentation] Improvement of crystal quality for tetragonal hen egg white lyzozyme crystals under application of alternative current electric field.2013

    • Author(s)
      H.Koizumi, S.Uda, K.Fujiwara,M.Tachibana, K.Kojima, J.Nozawa
    • Organizer
      17th International Conference on Crystal Growth and Eptaxy.
    • Place of Presentation
      ワルシャワ(ポーランド)
    • Related Report
      2013 Research-status Report
  • [Book] タンパク質結晶の最前線2013

    • Author(s)
      橘 勝 小島謙一
    • Total Pages
      10
    • Publisher
      シーエムシー出版
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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